PMID- 28577538 OWN - NLM STAT- MEDLINE DCOM- 20170821 LR - 20220316 IS - 1472-6882 (Electronic) IS - 1472-6882 (Linking) VI - 17 IP - 1 DP - 2017 Jun 2 TI - Dai-Huang-Fu-Zi-Tang alleviates pulmonary and intestinal injury with severe acute pancreatitis via regulating aquaporins in rats. PG - 288 LID - 10.1186/s12906-017-1789-x [doi] LID - 288 AB - BACKGROUND: Dai-Huang-Fu-Zi-Tang (DHFZT) is a famous traditional Chinese prescription with intestinal obstruction, acute pancreatitis and cholecystalgia for thousands of years. Our previous work found that DHFZT could act against pulmonary and intestinal pathological injury in rats with severe acute pancreatitis (SAP). But the underlying mechanism has not been fully elucidated. The aim of present study was to investigate whether DHFZT could relieve pulmonary and intestinal injury by regulating aquaporins after SAP induced by sodium taurocholate in rats. METHODS: Forty of SD rats were used for dose dependant experiments of DHFZT.Accurate-mass Time-of-flight liquid chromatography-mass spectrometry was used for qualitative screening of chemical compositions of DHFZT. Twenty-four rats were randomly divided into 3 groups: sham group (n = 8), model group (SAP, n = 8), DHFZT group (SAP with DHFZT treatment, n = 8). SAP models were established by retrograde injections of 5% sodium taurocholate solutions into rat pancreaticobiliary ducts. Blood samples were taken at 0, 12, 24, 48 h post-operation for detecting serum amylase, lipase, endotoxin, TNF-alpha, IL-6 and IL-10. Protein expression and location of aquaporin (AQP)1, 5, 8 and 9 were assessed by immunohistochemistry, western blot and immunofluorescence respectively. RESULTS: The study showed that 27 kinds of chemical composition were identified, including 10 kinds in positive ion mode and 17 kinds in negative ion mode. The results showed that AQP1, AQP5 of lung, and AQP1, AQP5, AQP8 of intestine in model group were significantly lower than that of sham group (P < 0.05), and which were obviously reversed by treatment with DHFZT. In addition, protein levels of pro-inflammatory cytokines such as TNF-alpha, IL-6 and endotoxin in peripheral blood were significantly suppressed by DHFZT, and that anti-inflammatory cytokine like IL-10 was just opposite. Finally, we also noted that DHFZT reduced serum levels of amylase, lipase and endotoxin, and also improved edema and pathological scores of lung and intestine after SAP. CONCLUSIONS: DHFZT ameliorated the pulmonary and intestinal edema and injury induced by SAP via the upregulation of different AQPs in lung and intestine, and suppressed TNF-alpha, IL-6 expression and enhanced IL-10 expression. FAU - Kang, Xin AU - Kang X AD - Department of Emergency Medicine, Zhongshan Hospital, Dalian University, Dalian, 116001, China. FAU - Lu, Xiao-Guang AU - Lu XG AD - Department of Emergency Medicine, Zhongshan Hospital, Dalian University, Dalian, 116001, China. dllxg@126.com. FAU - Zhan, Li-Bin AU - Zhan LB AD - Basic Medical College, Nanjing University of Chinese medicine, Nanjing, 210023, China. FAU - Liang, Zheng-Kai AU - Liang ZK AD - Department of Surgery, Liaocheng People's Hospital, Shandong, 300100, China. FAU - Guo, Wen-Xiu AU - Guo WX AD - Department of Emergency Medicine, Zhongshan Hospital, Dalian University, Dalian, 116001, China. FAU - Ma, Qi AU - Ma Q AD - Department of Emergency Medicine, Zhongshan Hospital, Dalian University, Dalian, 116001, China. FAU - Wang, Yi AU - Wang Y AD - Department of Emergency Medicine, Zhongshan Hospital, Dalian University, Dalian, 116001, China. AD - Graduate School, Dalian Medical University, Dalian, 116044, China. FAU - Song, Jian-Bo AU - Song JB AD - Department of Emergency Medicine, Zhongshan Hospital, Dalian University, Dalian, 116001, China. FAU - Feng, Jin-Yu AU - Feng JY AD - Department of Emergency Medicine, Zhongshan Hospital, Dalian University, Dalian, 116001, China. AD - Graduate School, Dalian Medical University, Dalian, 116044, China. FAU - Wang, Cong-Han AU - Wang CH AD - Department of Emergency Medicine, Zhongshan Hospital, Dalian University, Dalian, 116001, China. AD - Graduate School, Dalian Medical University, Dalian, 116044, China. FAU - Bai, Li-Zhi AU - Bai LZ AD - Department of Emergency Medicine, Zhongshan Hospital, Dalian University, Dalian, 116001, China. FAU - Song, Yi AU - Song Y AD - Department of Emergency Medicine, Zhongshan Hospital, Dalian University, Dalian, 116001, China. FAU - Liu, Guo-Hui AU - Liu GH AD - Department of Emergency Medicine, First Hospital of Jilin University, Changchun, 130012, China. zhangxx063@sina.com. LA - eng PT - Journal Article DEP - 20170602 PL - England TA - BMC Complement Altern Med JT - BMC complementary and alternative medicine JID - 101088661 RN - 0 (Aquaporins) RN - 0 (Drugs, Chinese Herbal) RN - 0 (Interleukin-6) RN - 130068-27-8 (Interleukin-10) SB - IM MH - Animals MH - Aquaporins/*genetics/metabolism MH - Drugs, Chinese Herbal/*administration & dosage MH - Humans MH - Interleukin-10/genetics/metabolism MH - Interleukin-6/genetics/metabolism MH - Intestinal Diseases/*drug therapy/etiology/genetics/metabolism MH - Intestinal Mucosa/metabolism MH - Intestines/injuries MH - Lung Injury/*drug therapy/etiology/genetics/metabolism MH - Male MH - Pancreatitis/*complications MH - Rats MH - Rats, Sprague-Dawley PMC - PMC5455207 OTO - NOTNLM OT - Aquaporin OT - Dai-Huang-Fu-Zi-Tang OT - Injury OT - Severe acute pancreatitis EDAT- 2017/06/05 06:00 MHDA- 2017/08/22 06:00 PMCR- 2017/06/02 CRDT- 2017/06/05 06:00 PHST- 2016/12/18 00:00 [received] PHST- 2017/05/11 00:00 [accepted] PHST- 2017/06/05 06:00 [entrez] PHST- 2017/06/05 06:00 [pubmed] PHST- 2017/08/22 06:00 [medline] PHST- 2017/06/02 00:00 [pmc-release] AID - 10.1186/s12906-017-1789-x [pii] AID - 1789 [pii] AID - 10.1186/s12906-017-1789-x [doi] PST - epublish SO - BMC Complement Altern Med. 2017 Jun 2;17(1):288. doi: 10.1186/s12906-017-1789-x.