PMID- 28578354
OWN - NLM
STAT- MEDLINE
DCOM- 20170804
LR  - 20220331
IS  - 1421-9778 (Electronic)
IS  - 1015-8987 (Linking)
VI  - 42
IP  - 2
DP  - 2017
TI  - Concurrent CCR7 Overexpression and RelB Knockdown in Immature Dendritic Cells 
      Induces Immune Tolerance and Improves Skin-Graft Survival in a Murine Model.
PG  - 455-468
LID - 10.1159/000477593 [doi]
AB  - BACKGROUND/AIMS: Skin transplantation aims to cover skin defects but often fails 
      due to immune rejection of the transplantated tissue. Immature dendritic cells 
      (imDCs) induce immune tolerance but have a low migration rate. After stimulation, 
      imDCs transform into mature DCs, which activate immune rejection. Thus, inducing 
      imDC to obtain a high migration counteracts development of immune tolerance. 
      METHODS & RESULTS: We transfected imDCs with a recombinant adenovirus carrying 
      the CCR7 gene (Ad-CCR7) and a small interfering RNA targeting RelB (RelB-siRNA) 
      to concurrently overexpress CCR7 and downregulate RelB expression. Functionally, 
      such cells showed a significantly enhanced migration rate in the chemotactic 
      assay and decreased T-cell proliferation after lipopolysaccharide stimulation in 
      mixed lymphocyte reactions. Cotransfected cells showed an increased ability to 
      induce immune tolerance by upregulating T regulatory (Treg) cells and shifting 
      the Th1/Th2 ratio. Cotransfection of Ad-CCR7 and RelB-siRNA endowed imDCs with 
      resistance to apoptosis and cell death. CCR7 overexpression and RelB knockdown 
      (KD) in imDCs improve skin-graft survival in a murine skin-transplantation model. 
      CONCLUSION: Transfection with Ad-CCR7 and RelB KD in imDCs may be an effective 
      approach inducing immune tolerance, thus being potentially valuable for 
      inhibiting allograft rejection.
CI  - (c) 2017 The Author(s). Published by S. Karger AG, Basel.
FAU - Dong, Zhiwei
AU  - Dong Z
AD  - Institute of Burn Research, Southwest Hospital, State Key Laboratory of Trauma, 
      Burns and Combined Injury, Third Military Medical University, Chongqing, China.
FAU - Chen, Yajie
AU  - Chen Y
AD  - Institute of Burn Research, Southwest Hospital, State Key Laboratory of Trauma, 
      Burns and Combined Injury, Third Military Medical University, Chongqing, China.
FAU - Peng, Yuan
AU  - Peng Y
AD  - The first clinical college of Chongqing Medical University, Chongqing, China.
FAU - Wang, Fan
AU  - Wang F
AD  - Department of Plastic and Reconstructive Surgery of Southwest Hospital, Third 
      Military Medical University Chongqing, Chongqing, China.
FAU - Yang, Zichen
AU  - Yang Z
AD  - Institute of Burn Research, Southwest Hospital, State Key Laboratory of Trauma, 
      Burns and Combined Injury, Third Military Medical University, Chongqing, China.
FAU - Huang, Guangtao
AU  - Huang G
AD  - Institute of Burn Research, Southwest Hospital, State Key Laboratory of Trauma, 
      Burns and Combined Injury, Third Military Medical University, Chongqing, China.
FAU - Chen, Yu
AU  - Chen Y
AD  - Institute of Burn Research, Southwest Hospital, State Key Laboratory of Trauma, 
      Burns and Combined Injury, Third Military Medical University, Chongqing, China.
FAU - Yuan, Zhiqiang
AU  - Yuan Z
AD  - Institute of Burn Research, Southwest Hospital, State Key Laboratory of Trauma, 
      Burns and Combined Injury, Third Military Medical University, Chongqing, China.
FAU - Cao, Tongtong
AU  - Cao T
AD  - Department of Pathology, Shanghai University of Traditional Chinese Medicine, 
      Shanghai, China.
FAU - Peng, Yizhi
AU  - Peng Y
AD  - Institute of Burn Research, Southwest Hospital, State Key Laboratory of Trauma, 
      Burns and Combined Injury, Third Military Medical University, Chongqing, China.
LA  - eng
PT  - Journal Article
DEP - 20170605
PL  - Germany
TA  - Cell Physiol Biochem
JT  - Cellular physiology and biochemistry : international journal of experimental 
      cellular physiology, biochemistry, and pharmacology
JID - 9113221
RN  - 0 (Ccr7 protein, mouse)
RN  - 0 (Receptors, CCR7)
RN  - 0 (Relb protein, mouse)
RN  - 147337-75-5 (Transcription Factor RelB)
SB  - IM
MH  - Adenoviridae
MH  - Animals
MH  - Dendritic Cells/immunology/metabolism
MH  - Gene Expression Regulation
MH  - Graft Rejection/genetics/immunology
MH  - Graft Survival/genetics
MH  - Humans
MH  - Immune Tolerance/*genetics
MH  - Mice
MH  - Receptors, CCR7/*biosynthesis/genetics
MH  - Skin/*immunology/metabolism
MH  - T-Lymphocytes, Regulatory/immunology/metabolism
MH  - Transcription Factor RelB/*genetics
MH  - Transfection
OTO - NOTNLM
OT  - CCR7
OT  - Immune tolerance
OT  - RelB
OT  - Transplantation
OT  - Treg cells
OT  - imDCs
EDAT- 2017/06/05 06:00
MHDA- 2017/08/05 06:00
CRDT- 2017/06/05 06:00
PHST- 2016/12/12 00:00 [received]
PHST- 2017/03/28 00:00 [accepted]
PHST- 2017/06/05 06:00 [pubmed]
PHST- 2017/08/05 06:00 [medline]
PHST- 2017/06/05 06:00 [entrez]
AID - 000477593 [pii]
AID - 10.1159/000477593 [doi]
PST - ppublish
SO  - Cell Physiol Biochem. 2017;42(2):455-468. doi: 10.1159/000477593. Epub 2017 Jun 
      5.