PMID- 28578959
OWN - NLM
STAT- MEDLINE
DCOM- 20180613
LR  - 20181202
IS  - 1879-1344 (Electronic)
IS  - 0144-8617 (Linking)
VI  - 171
DP  - 2017 Sep 1
TI  - Enzymatic production of N-acetyl-d-glucosamine from crayfish shell wastes 
      pretreated via high pressure homogenization.
PG  - 236-241
LID - S0144-8617(17)30535-0 [pii]
LID - 10.1016/j.carbpol.2017.05.028 [doi]
AB  - This study presents an efficient pretreatment of crayfish shell using high 
      pressure homogenization that enables N-acetyl-d-glucosamine (GlcNAc) production 
      by chitinase. Firstly, the chitinase from Serratia proteamaculans NJ303 was 
      screened for its ability to degrade crayfish shell and produce GlcNAc as the sole 
      product. Secondly, high pressure homogenization, which caused the crayfish shell 
      to adopt a fluffy netted structure that was characterized by Scanning electron 
      microscope (SEM), Fourier transform infrared spectrometer (FT-IR), X-ray 
      diffraction (XRD), was evaluated as the best pretreatment method. In addition, 
      the optimal conditions of high pressure homogenization of crayfish shell were 
      determined to be five cycles at a pressure of 400bar, which achieved a yield of 
      3.9g/L of GlcNAc from 25g/L of crayfish shell in a batch enzymatic reaction over 
      1.5h. The results showed high pressure homogenization might be an efficient 
      method for direct utilization of crayfish shell for enzymatic production of 
      GlcNAc.
CI  - Copyright (c) 2017 Elsevier Ltd. All rights reserved.
FAU - Wei, Guoguang
AU  - Wei G
AD  - State Key Laboratory of Materials-Oriented Chemical Engineering, College of 
      Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing 
      211816, PR China. Electronic address: wgg6164@njtech.edu.cn.
FAU - Zhang, Alei
AU  - Zhang A
AD  - State Key Laboratory of Materials-Oriented Chemical Engineering, College of 
      Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing 
      211816, PR China. Electronic address: 709131356@qq.com.
FAU - Chen, Kequan
AU  - Chen K
AD  - State Key Laboratory of Materials-Oriented Chemical Engineering, College of 
      Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing 
      211816, PR China. Electronic address: kqchen@njtech.edu.cn.
FAU - Ouyang, Pingkai
AU  - Ouyang P
AD  - State Key Laboratory of Materials-Oriented Chemical Engineering, College of 
      Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing 
      211816, PR China. Electronic address: 67420687@qq.com.
LA  - eng
PT  - Journal Article
DEP - 20170510
PL  - England
TA  - Carbohydr Polym
JT  - Carbohydrate polymers
JID - 8307156
RN  - EC 3.2.1.14 (Chitinases)
RN  - V956696549 (Acetylglucosamine)
SB  - IM
MH  - Acetylglucosamine/*chemical synthesis
MH  - Animals
MH  - Astacoidea/*chemistry
MH  - Biotechnology/*methods
MH  - Chitinases/*metabolism
MH  - *Pressure
OTO - NOTNLM
OT  - Chitin
OT  - Chitinase
OT  - Crayfish shells
OT  - High pressure homogenization
OT  - N-Acetyl-d-glucosamine
EDAT- 2017/06/06 06:00
MHDA- 2018/06/14 06:00
CRDT- 2017/06/06 06:00
PHST- 2017/03/09 00:00 [received]
PHST- 2017/05/05 00:00 [revised]
PHST- 2017/05/08 00:00 [accepted]
PHST- 2017/06/06 06:00 [entrez]
PHST- 2017/06/06 06:00 [pubmed]
PHST- 2018/06/14 06:00 [medline]
AID - S0144-8617(17)30535-0 [pii]
AID - 10.1016/j.carbpol.2017.05.028 [doi]
PST - ppublish
SO  - Carbohydr Polym. 2017 Sep 1;171:236-241. doi: 10.1016/j.carbpol.2017.05.028. Epub 
      2017 May 10.