PMID- 28579894 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20200929 IS - 1319-0164 (Print) IS - 2213-7475 (Electronic) IS - 1319-0164 (Linking) VI - 25 IP - 4 DP - 2017 May TI - Osteogenic differentiation of bone marrow mesenchymal stem cells by magnetic nanoparticle composite scaffolds under a pulsed electromagnetic field. PG - 575-579 LID - 10.1016/j.jsps.2017.04.026 [doi] AB - This study was conducted to investigate the effect of magnetic nanoparticle composite scaffold under a pulsed electromagnetic field on bone marrow mesenchymal stem cells (BMSCs), which was achieved by examining the biological behaviors of cell adhesion, proliferation and differentiation on the surface of the scaffolds. This may provide some experimental evidence for the use of magnetic nanoparticles in medical application. The magnetic nanoparticle composite scaffolds were evaluated and characterized by the following indexes: the cell proliferation was detected by the CCK-8 method, the alkaline phosphatase (ALP) activity was examined by a detection kit, and the expression of type I collagen and osteocalcin gene were evaluated by RT-PCR. The CCK-8 test showed that there was no significant difference in Group A (BMSCs-seeded magnetic scaffolds under the electromagnetic field), B (BMSCs-seeded magnetic scaffolds) and C (BMSCs cultured alone) (P > 0.05). The value for the ALP activity in Group A was higher than the other two groups. In addition, the RT-PCR results showed that the expression of type I collagen gene in Group A was enhanced (P < 0.05), suggesting that the magnetic nanoparticles combined with the pulsed electromagnetic field had a positive effect on the osteogenic differentiation of BMSCs. However, the expression of osteocalcin was not significantly different in three groups (P > 0.05). To conclude, magnetic nanoparticles may induce the osteogenic differentiation with the action of the pulsed electromagnetic field. FAU - Huang, Jianghong AU - Huang J AD - Shenzhen National Key Department of Orthopedics, Shenzhen Second People's Hospital, Shenzhen 518035, China. AD - Shenzhen Key Laboratory of Tissue Engineering, Shenzhen Second People's Hospital, Shenzhen 518035, China. AD - Shenzhen Laboratory of Digital Orthopedic Engineering, Shenzhen Second People's Hospital, Shenzhen 518035, China. FAU - Wang, Daming AU - Wang D AD - Shenzhen National Key Department of Orthopedics, Shenzhen Second People's Hospital, Shenzhen 518035, China. AD - Shenzhen Key Laboratory of Tissue Engineering, Shenzhen Second People's Hospital, Shenzhen 518035, China. AD - Shenzhen Laboratory of Digital Orthopedic Engineering, Shenzhen Second People's Hospital, Shenzhen 518035, China. AD - Software Town of Shenzhen Universiade, Shenzhen Longer3d Technology, Shenzhen 518116, China. FAU - Chen, Jielin AU - Chen J AD - Shenzhen National Key Department of Orthopedics, Shenzhen Second People's Hospital, Shenzhen 518035, China. AD - Shenzhen Key Laboratory of Tissue Engineering, Shenzhen Second People's Hospital, Shenzhen 518035, China. AD - Shenzhen Laboratory of Digital Orthopedic Engineering, Shenzhen Second People's Hospital, Shenzhen 518035, China. FAU - Liu, Wei AU - Liu W AD - Shenzhen National Key Department of Orthopedics, Shenzhen Second People's Hospital, Shenzhen 518035, China. AD - Shenzhen Key Laboratory of Tissue Engineering, Shenzhen Second People's Hospital, Shenzhen 518035, China. AD - Shenzhen Laboratory of Digital Orthopedic Engineering, Shenzhen Second People's Hospital, Shenzhen 518035, China. FAU - Duan, Li AU - Duan L AD - Shenzhen National Key Department of Orthopedics, Shenzhen Second People's Hospital, Shenzhen 518035, China. AD - Shenzhen Key Laboratory of Tissue Engineering, Shenzhen Second People's Hospital, Shenzhen 518035, China. AD - Shenzhen Laboratory of Digital Orthopedic Engineering, Shenzhen Second People's Hospital, Shenzhen 518035, China. FAU - You, Wei AU - You W AD - Shenzhen National Key Department of Orthopedics, Shenzhen Second People's Hospital, Shenzhen 518035, China. AD - Shenzhen Key Laboratory of Tissue Engineering, Shenzhen Second People's Hospital, Shenzhen 518035, China. AD - Shenzhen Laboratory of Digital Orthopedic Engineering, Shenzhen Second People's Hospital, Shenzhen 518035, China. FAU - Zhu, Weimin AU - Zhu W AD - Shenzhen National Key Department of Orthopedics, Shenzhen Second People's Hospital, Shenzhen 518035, China. AD - Shenzhen Key Laboratory of Tissue Engineering, Shenzhen Second People's Hospital, Shenzhen 518035, China. AD - Shenzhen Laboratory of Digital Orthopedic Engineering, Shenzhen Second People's Hospital, Shenzhen 518035, China. FAU - Xiong, Jianyi AU - Xiong J AD - Shenzhen National Key Department of Orthopedics, Shenzhen Second People's Hospital, Shenzhen 518035, China. AD - Shenzhen Key Laboratory of Tissue Engineering, Shenzhen Second People's Hospital, Shenzhen 518035, China. AD - Shenzhen Laboratory of Digital Orthopedic Engineering, Shenzhen Second People's Hospital, Shenzhen 518035, China. FAU - Wang, Daping AU - Wang D AD - Shenzhen National Key Department of Orthopedics, Shenzhen Second People's Hospital, Shenzhen 518035, China. AD - Shenzhen Key Laboratory of Tissue Engineering, Shenzhen Second People's Hospital, Shenzhen 518035, China. AD - Shenzhen Laboratory of Digital Orthopedic Engineering, Shenzhen Second People's Hospital, Shenzhen 518035, China. LA - eng PT - Journal Article DEP - 20170508 PL - Saudi Arabia TA - Saudi Pharm J JT - Saudi pharmaceutical journal : SPJ : the official publication of the Saudi Pharmaceutical Society JID - 9705695 PMC - PMC5447436 OTO - NOTNLM OT - Bone marrow mesenchymal stem cells OT - Magnetic nanoparticles OT - Osteogenic differentiation OT - Pulsed electromagnetic field EDAT- 2017/06/06 06:00 MHDA- 2017/06/06 06:01 PMCR- 2017/05/08 CRDT- 2017/06/06 06:00 PHST- 2017/06/06 06:00 [entrez] PHST- 2017/06/06 06:00 [pubmed] PHST- 2017/06/06 06:01 [medline] PHST- 2017/05/08 00:00 [pmc-release] AID - S1319-0164(17)30091-9 [pii] AID - 10.1016/j.jsps.2017.04.026 [doi] PST - ppublish SO - Saudi Pharm J. 2017 May;25(4):575-579. doi: 10.1016/j.jsps.2017.04.026. Epub 2017 May 8.