PMID- 28583077
OWN - NLM
STAT- MEDLINE
DCOM- 20180122
LR  - 20181113
IS  - 1471-2164 (Electronic)
IS  - 1471-2164 (Linking)
VI  - 18
IP  - 1
DP  - 2017 Jun 5
TI  - Identification of conserved genes triggering puberty in European sea bass males 
      (Dicentrarchus labrax) by microarray expression profiling.
PG  - 441
LID - 10.1186/s12864-017-3823-2 [doi]
LID - 441
AB  - BACKGROUND: Spermatogenesis is a complex process characterized by the activation 
      and/or repression of a number of genes in a spatio-temporal manner. Pubertal 
      development in males starts with the onset of the first spermatogenesis and 
      implies the division of primary spermatogonia and their subsequent entry into 
      meiosis. This study is aimed at the characterization of genes involved in the 
      onset of puberty in European sea bass, and constitutes the first transcriptomic 
      approach focused on meiosis in this species. RESULTS: European sea bass testes 
      collected at the onset of puberty (first successful reproduction) were grouped in 
      stage I (resting stage), and stage II (proliferative stage). Transition from 
      stage I to stage II was marked by an increase of 11ketotestosterone (11KT), the 
      main fish androgen, whereas the transcriptomic study resulted in 315 genes 
      differentially expressed between the two stages. The onset of puberty induced 1) 
      an up-regulation of genes involved in cell proliferation, cell cycle and meiosis 
      progression, 2) changes in genes related with reproduction and growth, and 3) a 
      down-regulation of genes included in the retinoic acid (RA) signalling pathway. 
      The analysis of GO-terms and biological pathways showed that cell cycle, cell 
      division, cellular metabolic processes, and reproduction were affected, 
      consistent with the early events that occur during the onset of puberty. 
      Furthermore, changes in the expression of three RA nuclear receptors point at the 
      importance of the RA-signalling pathway during this period, in agreement with its 
      role in meiosis. CONCLUSION: The results contribute to boost our knowledge of the 
      early molecular and endocrine events that trigger pubertal development and the 
      onset of spermatogenesis in fish. These include an increase in 11KT plasma levels 
      and changes in the expression of several genes involved in cell proliferation, 
      cell cycle progression, meiosis or RA-signalling pathway. Moreover, the results 
      can be applied to study meiosis in this economically important fish species for 
      Mediterranean countries, and may help to develop tools for its sustainable 
      aquaculture.
FAU - Blazquez, Mercedes
AU  - Blazquez M
AUID- ORCID: 0000-0003-0522-2107
AD  - Instituto de Acuicultura de Torre la Sal, Consejo Superior de Investigaciones 
      Cientificas (IATS-CSIC), Ribera de Cabanes, 12595, Castellon, Spain. 
      blazquez@icm.csic.es.
AD  - Instituto de Ciencias del Mar, Consejo Superior de Investigaciones Cientificas 
      (ICM-CSIC), Passeig Maritim 37-49, 08003, Barcelona, Spain. blazquez@icm.csic.es.
FAU - Medina, Paula
AU  - Medina P
AD  - Instituto de Acuicultura de Torre la Sal, Consejo Superior de Investigaciones 
      Cientificas (IATS-CSIC), Ribera de Cabanes, 12595, Castellon, Spain.
AD  - Instituto de Ciencias del Mar, Consejo Superior de Investigaciones Cientificas 
      (ICM-CSIC), Passeig Maritim 37-49, 08003, Barcelona, Spain.
AD  - Present address: Universidad de Antofagasta, Avda Angamos 601, Antofagasta, 
      Chile.
FAU - Crespo, Berta
AU  - Crespo B
AD  - Instituto de Acuicultura de Torre la Sal, Consejo Superior de Investigaciones 
      Cientificas (IATS-CSIC), Ribera de Cabanes, 12595, Castellon, Spain.
AD  - Present address: UCL Great Ormond Street Institute of Child Health, 30 Guilford 
      Street, London, WC1N 1EH, UK.
FAU - Gomez, Ana
AU  - Gomez A
AD  - Instituto de Acuicultura de Torre la Sal, Consejo Superior de Investigaciones 
      Cientificas (IATS-CSIC), Ribera de Cabanes, 12595, Castellon, Spain.
FAU - Zanuy, Silvia
AU  - Zanuy S
AD  - Instituto de Acuicultura de Torre la Sal, Consejo Superior de Investigaciones 
      Cientificas (IATS-CSIC), Ribera de Cabanes, 12595, Castellon, Spain. 
      s.zanuy@csic.es.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20170605
PL  - England
TA  - BMC Genomics
JT  - BMC genomics
JID - 100965258
RN  - 0 (Fish Proteins)
RN  - 0 (Hormones)
SB  - IM
EIN - BMC Genomics. 2017 Jul 5;18(1):513. PMID: 28679394
MH  - Animals
MH  - Bass/*genetics/metabolism/*physiology
MH  - Cloning, Molecular
MH  - *Conserved Sequence
MH  - Fish Proteins/*genetics
MH  - *Gene Expression Profiling
MH  - Gene Ontology
MH  - Hormones/metabolism
MH  - Male
MH  - *Oligonucleotide Array Sequence Analysis
MH  - Phylogeny
MH  - Puberty/*genetics
PMC - PMC5460432
OTO - NOTNLM
OT  - Cell cycle
OT  - Meiosis
OT  - Retinoic acid
OT  - Spermatogenesis
OT  - Teleosts
EDAT- 2017/06/07 06:00
MHDA- 2018/01/23 06:00
PMCR- 2017/06/05
CRDT- 2017/06/07 06:00
PHST- 2016/11/07 00:00 [received]
PHST- 2017/05/25 00:00 [accepted]
PHST- 2017/06/07 06:00 [entrez]
PHST- 2017/06/07 06:00 [pubmed]
PHST- 2018/01/23 06:00 [medline]
PHST- 2017/06/05 00:00 [pmc-release]
AID - 10.1186/s12864-017-3823-2 [pii]
AID - 3823 [pii]
AID - 10.1186/s12864-017-3823-2 [doi]
PST - epublish
SO  - BMC Genomics. 2017 Jun 5;18(1):441. doi: 10.1186/s12864-017-3823-2.