PMID- 28583105
OWN - NLM
STAT- MEDLINE
DCOM- 20180320
LR  - 20220410
IS  - 1471-2474 (Electronic)
IS  - 1471-2474 (Linking)
VI  - 18
IP  - 1
DP  - 2017 Jun 5
TI  - Characteristics and potentials of stem cells derived from human degenerated 
      nucleus pulposus: potential for regeneration of the intervertebral disc.
PG  - 242
LID - 10.1186/s12891-017-1567-4 [doi]
LID - 242
AB  - BACKGROUND: Eliminating the symptoms during treatment of intervertebral disc 
      degeneration (IVDD) is only a temporary solution that does not cure the 
      underlying cause. A biological method to treat this disorder may be possible by 
      the newly discovered nucleus pulposus derived stem cells (NPDCs). However, the 
      uncertain characteristics and potential of NPDCs calls for a comprehensive study. 
      METHODS: In the present study, nucleus pulposus samples were obtained from 5 
      patients with IVDD undergoing discectomy procedure and NPDCs were harvested using 
      fluorescence activated cell sorting (FACS) by the co-expression of GD2(+) and 
      Tie2(+). After in vitro expansion, the properties of NPDCs were compared with 
      those of bone marrow mesenchyme stem cells (BMSCs) from the same subjects. 
      RESULTS: NPDCs performed similar properties in cell colony-forming ability, cell 
      proliferation rate, cell cycle and stem cell gene expression similar to those of 
      BMSCs. In addition, NPDCs could be differentiated into osteoblasts, adipocytes, 
      and chondrocytes, and are found to be superior in chondrogenesis but inferior in 
      adipocyte differentiation. CONCLUSIONS: NPDCs derived from the degenerated 
      intervertebral disc still keep the regeneration ability similar to BMSCs. 
      Besides, the superior capacity in chondrogenesis may provide a promising cell 
      candidate for cell-based regenerative medicine and tissue engineering in IVDD.
FAU - Li, Xiao-Chuan
AU  - Li XC
AD  - Department of Orthopaedic Surgery, Navy General Hospital, Beijing, 100048, China.
AD  - Department of Orthopedic Surgery, Gaozhou people's Hospital, No 89, Xi-Guan Road, 
      Guangdong, Guangzhou, 525200, China.
FAU - Tang, Yong
AU  - Tang Y
AD  - Department of Orthopaedic Surgery, Navy General Hospital, Beijing, 100048, China.
FAU - Wu, Jian-Hong
AU  - Wu JH
AD  - Department of Orthopaedic Surgery, Navy General Hospital, Beijing, 100048, China.
FAU - Yang, Pu-Shan
AU  - Yang PS
AD  - Department of Orthopaedic Surgery, Navy General Hospital, Beijing, 100048, China.
FAU - Wang, De-Li
AU  - Wang DL
AD  - Department of Orthopaedic Surgery, Navy General Hospital, Beijing, 100048, China. 
      wangdelinavy@163.com.
FAU - Ruan, Di-Ke
AU  - Ruan DK
AD  - Department of Orthopaedic Surgery, Navy General Hospital, Beijing, 100048, China. 
      ruandikengh@163.com.
LA  - eng
PT  - Journal Article
PT  - Multicenter Study
DEP - 20170605
PL  - England
TA  - BMC Musculoskelet Disord
JT  - BMC musculoskeletal disorders
JID - 100968565
SB  - IM
MH  - Cell Differentiation/physiology
MH  - Cells, Cultured
MH  - Female
MH  - Humans
MH  - Intervertebral Disc/pathology/*physiology
MH  - Intervertebral Disc Degeneration/*pathology
MH  - Male
MH  - Mesenchymal Stem Cells/physiology
MH  - Middle Aged
MH  - Nucleus Pulposus/pathology/*physiology/transplantation
MH  - Regeneration/*physiology
MH  - Stem Cells/*pathology/*physiology
PMC - PMC5460486
OTO - NOTNLM
OT  - Biological characteristics
OT  - Cell therapy
OT  - Endogenous repair
OT  - Intervertebral disc
OT  - Nucleus pulposus derived stem cells
EDAT- 2017/06/07 06:00
MHDA- 2018/03/21 06:00
PMCR- 2017/06/05
CRDT- 2017/06/07 06:00
PHST- 2016/11/18 00:00 [received]
PHST- 2017/05/09 00:00 [accepted]
PHST- 2017/06/07 06:00 [entrez]
PHST- 2017/06/07 06:00 [pubmed]
PHST- 2018/03/21 06:00 [medline]
PHST- 2017/06/05 00:00 [pmc-release]
AID - 10.1186/s12891-017-1567-4 [pii]
AID - 1567 [pii]
AID - 10.1186/s12891-017-1567-4 [doi]
PST - epublish
SO  - BMC Musculoskelet Disord. 2017 Jun 5;18(1):242. doi: 10.1186/s12891-017-1567-4.