PMID- 28583133
OWN - NLM
STAT- MEDLINE
DCOM- 20180214
LR  - 20181113
IS  - 1475-2875 (Electronic)
IS  - 1475-2875 (Linking)
VI  - 16
IP  - 1
DP  - 2017 Jun 5
TI  - Comparative physical genome mapping of malaria vectors Anopheles sinensis and 
      Anopheles gambiae.
PG  - 235
LID - 10.1186/s12936-017-1888-7 [doi]
LID - 235
AB  - BACKGROUND: Anopheles sinensis is a dominant natural vector of Plasmodium vivax 
      in China, Taiwan, Japan, and Korea. Recent genome sequencing of An. sinensis 
      provides important insights into the genomic basis of vectorial capacity. 
      However, the lack of a physical genome map with chromosome assignment and 
      orientation of sequencing scaffolds hinders comparative analyses with other 
      genomes to infer evolutionary changes relevant to the vector capacity. RESULTS: 
      Here, a physical genome map for An. sinensis was constructed by assigning 52 
      scaffolds onto the chromosomes using fluorescence in situ hybridization (FISH). 
      This chromosome-based genome assembly composes approximately 36% of the total An. 
      sinensis genome. Comparisons of 3955 orthologous genes between An. sinensis and 
      Anopheles gambiae identified 361 conserved synteny blocks and 267 inversions 
      fixed between these two lineages. The rate of gene order reshuffling on the X 
      chromosome is approximately 3.2 times higher than that on the autosomes. 
      CONCLUSIONS: The physical map will facilitate detailed genomic analysis of An. 
      sinensis and contribute to understanding of the patterns and mechanisms of 
      large-scale genome rearrangements in anopheline mosquitoes.
FAU - Wei, Yun
AU  - Wei Y
AD  - Department of Entomology, Nanjing Agricultural University, Nanjing, China.
FAU - Cheng, Biao
AU  - Cheng B
AD  - Department of Entomology, Nanjing Agricultural University, Nanjing, China.
FAU - Zhu, Guoding
AU  - Zhu G
AD  - Key Laboratory of National Health and Family Planning Commission on Parasitic 
      Disease Control and Prevention, Jiangsu Provincial Key Laboratory on Parasite and 
      Vector Control Technology, Jiangsu Institute of Parasitic Diseases, Wuxi, Jiangsu 
      Province, China.
FAU - Shen, Danyu
AU  - Shen D
AD  - Department of Entomology, Nanjing Agricultural University, Nanjing, China.
FAU - Liang, Jiangtao
AU  - Liang J
AD  - Department of Entomology, Nanjing Agricultural University, Nanjing, China.
FAU - Wang, Cong
AU  - Wang C
AD  - Department of Entomology, Nanjing Agricultural University, Nanjing, China.
FAU - Wang, Jing
AU  - Wang J
AD  - Department of Entomology, Nanjing Agricultural University, Nanjing, China.
FAU - Tang, Jianxia
AU  - Tang J
AD  - Key Laboratory of National Health and Family Planning Commission on Parasitic 
      Disease Control and Prevention, Jiangsu Provincial Key Laboratory on Parasite and 
      Vector Control Technology, Jiangsu Institute of Parasitic Diseases, Wuxi, Jiangsu 
      Province, China.
FAU - Cao, Jun
AU  - Cao J
AD  - Key Laboratory of National Health and Family Planning Commission on Parasitic 
      Disease Control and Prevention, Jiangsu Provincial Key Laboratory on Parasite and 
      Vector Control Technology, Jiangsu Institute of Parasitic Diseases, Wuxi, Jiangsu 
      Province, China.
FAU - Sharakhov, Igor V
AU  - Sharakhov IV
AD  - Department of Entomology, Fralin Life Science Institute, Virginia Tech, 
      Blacksburg, VA, USA.
AD  - Laboratory for Ecology, Genetics and Environmental Protection, Tomsk State 
      University, Tomsk, Russia.
FAU - Xia, Ai
AU  - Xia A
AUID- ORCID: 0000-0003-0373-9196
AD  - Department of Entomology, Nanjing Agricultural University, Nanjing, China. 
      xiaai@njau.edu.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20170605
PL  - England
TA  - Malar J
JT  - Malaria journal
JID - 101139802
SB  - IM
MH  - Animals
MH  - Anopheles/*genetics
MH  - Chromosome Inversion/genetics
MH  - Chromosome Mapping
MH  - *Genome, Insect
MH  - In Situ Hybridization, Fluorescence
MH  - Malaria
MH  - Mosquito Vectors/*genetics
MH  - Polytene Chromosomes/genetics
MH  - Sex Chromosomes/genetics
PMC - PMC5460330
OTO - NOTNLM
OT  - Chromosomal evolution
OT  - Fluorescence in situ hybridization
OT  - GRIMM
OT  - Inversion fixation
OT  - OrthoDB
OT  - Synteny blocks
EDAT- 2017/06/07 06:00
MHDA- 2018/02/15 06:00
PMCR- 2017/06/05
CRDT- 2017/06/07 06:00
PHST- 2017/02/08 00:00 [received]
PHST- 2017/05/31 00:00 [accepted]
PHST- 2017/06/07 06:00 [entrez]
PHST- 2017/06/07 06:00 [pubmed]
PHST- 2018/02/15 06:00 [medline]
PHST- 2017/06/05 00:00 [pmc-release]
AID - 10.1186/s12936-017-1888-7 [pii]
AID - 1888 [pii]
AID - 10.1186/s12936-017-1888-7 [doi]
PST - epublish
SO  - Malar J. 2017 Jun 5;16(1):235. doi: 10.1186/s12936-017-1888-7.