PMID- 28584576
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200929
IS  - 1976-1457 (Print)
IS  - 2005-6168 (Electronic)
IS  - 1976-1457 (Linking)
VI  - 11
IP  - 3
DP  - 2017 Jun
TI  - Pear pomace ethanol extract improves insulin resistance through enhancement of 
      insulin signaling pathway without lipid accumulation.
PG  - 198-205
LID - 10.4162/nrp.2017.11.3.198 [doi]
AB  - BACKGROUND/OBJECTIVES: The anti-diabetic activity of pear through inhibition of 
      alpha-glucosidase has been demonstrated. However, little has been reported about the 
      effect of pear on insulin signaling pathway in obesity. The aims of this study 
      are to establish pear pomace 50% ethanol extract (PPE)-induced improvement of 
      insulin sensitivity and characterize its action mechanism in 3T3-L1 cells and 
      high-fat diet (HFD)-fed C57BL/6 mice. MATERIALS/METHODS: Lipid accumulation, 
      monocyte chemoattractant protein-1 (MCP-1) secretion and glucose uptake were 
      measure in 3T3-L1 cells. Mice were fed HFD (60% kcal from fat) and orally 
      ingested PPE once daily for 8 weeks and body weight, homeostasis model assessment 
      of insulin resistance (HOMA-IR), and serum lipids were measured. The expression 
      of proteins involved in insulin signaling pathway was evaluated by western blot 
      assay in 3T3-L1 cells and adipose tissue of mice. RESULTS: In 3T3-L1 cells, 
      without affecting cell viability and lipid accumulation, PPE inhibited MCP-1 
      secretion, improved glucose uptake, and increased protein expression of 
      phosphorylated insulin receptor substrate 1 [p-IRS-1, (Tyr(632))], p-Akt, and 
      glucose transporter type 4 (GLUT4). Additionally, in HFD-fed mice, PPE reduced 
      body weight, HOMA-IR, and serum lipids including triglyceride and 
      LDL-cholesterol. Furthermore, in adipose tissue, PPE up-regulated GLUT4 
      expression and expression ratio of p-IRS-1 (Tyr(632))/IRS, whereas, 
      down-regulated p-IRS-1 (Ser(307))/IRS. CONCLUSIONS: Our results collectively show 
      that PPE improves glucose uptake in 3T3-L1 cells and insulin sensitivity in mice 
      fed a HFD through stimulation of the insulin signaling pathway. Furthermore, 
      PPE-induced improvement of insulin sensitivity was not accompanied with lipid 
      accumulation.
FAU - You, Mi-Kyoung
AU  - You MK
AD  - Department of Food and Nutrition / Research Institute of Human Ecology, Mokpo 
      National University, 1666, Yeongsan-ro, Cheonggye-myeon, Muan-gun, Jeonam 58554, 
      Korea.
FAU - Kim, Hwa-Jin
AU  - Kim HJ
AD  - Department of Food and Nutrition / Research Institute of Human Ecology, Mokpo 
      National University, 1666, Yeongsan-ro, Cheonggye-myeon, Muan-gun, Jeonam 58554, 
      Korea.
FAU - Rhyu, Jin
AU  - Rhyu J
AD  - Department of Food and Nutrition / Research Institute of Human Ecology, Mokpo 
      National University, 1666, Yeongsan-ro, Cheonggye-myeon, Muan-gun, Jeonam 58554, 
      Korea.
FAU - Kim, Hyeon-A
AU  - Kim HA
AD  - Department of Food and Nutrition / Research Institute of Human Ecology, Mokpo 
      National University, 1666, Yeongsan-ro, Cheonggye-myeon, Muan-gun, Jeonam 58554, 
      Korea.
LA  - eng
PT  - Journal Article
DEP - 20170409
PL  - Korea (South)
TA  - Nutr Res Pract
JT  - Nutrition research and practice
JID - 101311052
PMC - PMC5449376
OTO - NOTNLM
OT  - Insulin sensitivity
OT  - adipose tissue
OT  - glucose transporter
OT  - lipid metabolism
OT  - pyrus
COIS- CONFLICT OF INTEREST: The authors declare no potential conflicts of interests.
EDAT- 2017/06/07 06:00
MHDA- 2017/06/07 06:01
PMCR- 2017/06/01
CRDT- 2017/06/07 06:00
PHST- 2016/10/31 00:00 [received]
PHST- 2016/11/29 00:00 [revised]
PHST- 2017/02/07 00:00 [accepted]
PHST- 2017/06/07 06:00 [entrez]
PHST- 2017/06/07 06:00 [pubmed]
PHST- 2017/06/07 06:01 [medline]
PHST- 2017/06/01 00:00 [pmc-release]
AID - 10.4162/nrp.2017.11.3.198 [doi]
PST - ppublish
SO  - Nutr Res Pract. 2017 Jun;11(3):198-205. doi: 10.4162/nrp.2017.11.3.198. Epub 2017 
      Apr 9.