PMID- 28584985
OWN - NLM
STAT- MEDLINE
DCOM- 20180326
LR  - 20180705
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 1625
DP  - 2017
TI  - DNAhsp65 Vaccine as Therapy against Paracoccidioidomycosis.
PG  - 85-96
LID - 10.1007/978-1-4939-7104-6_7 [doi]
AB  - The conventional treatment for fungal diseases usually shows long periods of 
      therapy and the high frequency of relapses and sequels. New strategies of the 
      treatment are necessary. We have shown that the Mycobacterium leprae HSP65 gene 
      can be successfully used as therapy against murine Paracoccidioidomycosis (PCM). 
      Here, we described the methodology of DNAhsp65 immunotherapy in mice infected 
      with the dimorphic fungus Paracoccidioides brasiliensis, one of PCM agent, 
      evaluating cytokines levels, fungal burden, and lung injury. Our results provide 
      a new prospective on the immunotherapy of mycosis.
FAU - Ribeiro, Alice M
AU  - Ribeiro AM
AD  - Institute of Biological Sciences, University of Brasilia, Brasilia, Brazil.
FAU - Amaral, Andre C
AU  - Amaral AC
AD  - Tropical Pathology and Public Health Institute, Federal University of Goias, 
      Goiania, Brazil.
FAU - Felipe, Maria Sueli S
AU  - Felipe MSS
AD  - Institute of Biological Sciences, University of Brasilia, Brasilia, Brazil.
AD  - Genomic Science and Biotechnology, Catholic University of Brasilia, Brasilia, 
      Brazil.
FAU - Bocca, Anamelia L
AU  - Bocca AL
AD  - Institute of Biological Sciences, University of Brasilia, Brasilia, Brazil. 
      albocca@unb.br.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
RN  - 0 (Antibodies)
RN  - 0 (Antigens, Bacterial)
RN  - 0 (Bacterial Proteins)
RN  - 0 (Chaperonin 60)
RN  - 0 (Cytokines)
RN  - 0 (Fungal Vaccines)
RN  - 0 (Vaccines, DNA)
RN  - 0 (heat-shock protein 65, Mycobacterium)
RN  - 31C4KY9ESH (Nitric Oxide)
SB  - IM
MH  - Animals
MH  - Antibodies/immunology
MH  - Antibody Specificity/immunology
MH  - Antigens, Bacterial/genetics/*immunology
MH  - Bacterial Proteins/genetics/*immunology
MH  - Chaperonin 60/genetics/*immunology
MH  - Cytokines/metabolism
MH  - Disease Models, Animal
MH  - Fungal Vaccines/genetics/*immunology
MH  - Immunotherapy/methods
MH  - Lymphocyte Activation/immunology
MH  - Mice
MH  - Nitric Oxide/metabolism
MH  - Paracoccidioidomycosis/*immunology/microbiology/prevention & control/therapy
MH  - Plasmids/genetics
MH  - Spleen/immunology/metabolism/pathology
MH  - Vaccines, DNA/genetics/*immunology
OTO - NOTNLM
OT  - DNA vaccine
OT  - HSP65
OT  - Immunotherapy
OT  - Paracoccidioidomycosis
OT  - Paracoccioides brasiliensis
OT  - Systemic mycosis
EDAT- 2017/06/07 06:00
MHDA- 2018/03/27 06:00
CRDT- 2017/06/07 06:00
PHST- 2017/06/07 06:00 [entrez]
PHST- 2017/06/07 06:00 [pubmed]
PHST- 2018/03/27 06:00 [medline]
AID - 10.1007/978-1-4939-7104-6_7 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2017;1625:85-96. doi: 10.1007/978-1-4939-7104-6_7.