PMID- 28584992
OWN - NLM
STAT- MEDLINE
DCOM- 20180326
LR  - 20180705
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 1625
DP  - 2017
TI  - Yeast Expressing Gp43 Protein as a Vaccine Against Paracoccidioides brasiliensis 
      Infection.
PG  - 213-224
LID - 10.1007/978-1-4939-7104-6_14 [doi]
AB  - Paracoccidioidomycosis (PCM) represents the most frequent systemic mycosis in 
      Latin American. The disease is caused by the pathogenic thermally dimorphic 
      fungus Paracoccidioides brasiliensis, and is initially characterized by pulmonary 
      lesions, which can subsequently disseminate to other organs, resulting in 
      secondary injuries. Although its high incidence, there is no commercially 
      available vaccine against fungal diseases. A novel strategy, using Saccharomyces 
      cerevisiae yeast as a vehicle for immunization against PCM, was recently 
      successfully described. Herein, we describe strategies for the construction of 
      the suitable S. cerevisiae vaccine, and protocols of administration and 
      evaluation of the efficacy of the vaccine against experimental PCM.
FAU - Oliveira, Aline Ferreira
AU  - Oliveira AF
AD  - Departamento de Biologia Celular e Molecular e Bioagentes Patogenicos, Faculdade 
      de Medicina de Ribeirao Preto, Universidade de Sao Paulo, Avenida dos 
      Bandeirantes 3900, Ribeirao Preto, SP, 14049-900, Brasil.
AD  - Universidade Paulista, Ribeirao Preto, SP, Brasil.
AD  - Universidade Paulista, Sao Jose do Rio Pardo, SP, Brasil.
FAU - Coelho, Paulo S R
AU  - Coelho PSR
AD  - Departamento de Biologia Celular e Molecular e Bioagentes Patogenicos, Faculdade 
      de Medicina de Ribeirao Preto, Universidade de Sao Paulo, Avenida dos 
      Bandeirantes 3900, Ribeirao Preto, SP, 14049-900, Brasil. pacoelho@fmrp.usp.br.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
RN  - 0 (43 kDa protein, Paracoccidioides)
RN  - 0 (Antigens, Fungal)
RN  - 0 (Cytokines)
RN  - 0 (Fungal Proteins)
RN  - 0 (Fungal Vaccines)
RN  - 0 (Glycoproteins)
RN  - 0 (Recombinant Proteins)
SB  - IM
MH  - Animals
MH  - Antigens, Fungal/*administration & dosage/*genetics/immunology
MH  - Cloning, Molecular
MH  - Cytokines/metabolism
MH  - Fungal Proteins/*genetics/*immunology
MH  - Fungal Vaccines/*immunology/therapeutic use
MH  - Gene Expression
MH  - Glycoproteins/*genetics/*immunology
MH  - Immunization
MH  - Immunotherapy
MH  - Mice
MH  - Open Reading Frames
MH  - Paracoccidioidomycosis/*immunology/microbiology/prevention & control/therapy
MH  - Recombinant Proteins/genetics/immunology
OTO - NOTNLM
OT  - Gp43 recombinant protein
OT  - Paracoccidioides brasiliensis
OT  - Paracoccidioidomycosis (PCM)
OT  - Saccharomyces cerevisiae
OT  - Vaccine
EDAT- 2017/06/07 06:00
MHDA- 2018/03/27 06:00
CRDT- 2017/06/07 06:00
PHST- 2017/06/07 06:00 [entrez]
PHST- 2017/06/07 06:00 [pubmed]
PHST- 2018/03/27 06:00 [medline]
AID - 10.1007/978-1-4939-7104-6_14 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2017;1625:213-224. doi: 10.1007/978-1-4939-7104-6_14.