PMID- 28586004
OWN - NLM
STAT- MEDLINE
DCOM- 20180530
LR  - 20220330
IS  - 1791-244X (Electronic)
IS  - 1107-3756 (Linking)
VI  - 40
IP  - 2
DP  - 2017 Aug
TI  - COX-2 regulates Snail expression in gastric cancer via the Notch1 signaling 
      pathway.
PG  - 512-522
LID - 10.3892/ijmm.2017.3011 [doi]
AB  - The conversion of arachidonic acid into prostaglandins by cyclooxygenase (COX)-2 
      contributes to the biological properties of malignant tumours. During the 
      initiation and development of various tumours, the Notch family plays a key role. 
      However, the association between COX‑2 and the Notch family in gastric 
      cancer (GC) remains unclear. The present study aimed to clarify the mechanisms 
      through which COX‑2 participates in the pathogenesis of GC. Quantitative PCR and 
      western blot analysis were used to detect the expression of Notch family members 
      and COX‑2 in human GC and paracancerous tissues, GES‑1 cells and GC cell lines 
      (AGS, SGC‑7901, BGC‑823, and MGC‑803) treated with or without celecoxib, 
      prostaglandin E2 and small interfering RNA (siRNA). A CCK‑8 assay was performed 
      to detect the proliferation of GC cells transfected with siRNA against 
      COX‑2 (si‑COX‑2). A high mRNA expression of Notch1 and a decreased expression of 
      Notch-1 intracellular active domain (N1IC) in GC were found to be related to the 
      depth of invasion and TNM staging. The mRNA levels of Notch2, Notch3, Jagged1 and 
      N2IC were found to be high in GC. A High expression of COX‑2 was associated with 
      poorly differentiated and deeply invasive GC. COX‑2 and Notch1 exhibited an 
      inverse expression pattern in the GES‑1 cells and different GC cell lines; the 
      inhibition of COX‑2 increased Notch1 expression and activated the GC cells, 
      whereas Notch1 downregulation had the opposite effect. Notch1 exhibited varying 
      effects on Snail in the GC cell lines. The downregulation of COX‑2 expression 
      significantly inhibited the proliferation of GC cells. On the whole, the 
      expression of Notch signalling molecules differed in GC. COX‑2 inversely 
      regulated Notch1 in GC and partially depended on the Notch1 signalling pathway in 
      altering the expression of Snail.
FAU - Ye, Yuwei
AU  - Ye Y
AD  - Division of Gastroenterology and Hepatology, The First Hospital of Lanzhou 
      University, Lanzhou, Gansu 730000, P.R. China.
FAU - Liu, Min
AU  - Liu M
AD  - Division of Gastroenterology and Hepatology, The First Hospital of Lanzhou 
      University, Lanzhou, Gansu 730000, P.R. China.
FAU - Yuan, Hao
AU  - Yuan H
AD  - Division of Gastroenterology and Hepatology, The First Hospital of Lanzhou 
      University, Lanzhou, Gansu 730000, P.R. China.
FAU - Ning, Shupeng
AU  - Ning S
AD  - Department of Anesthesiology, The Second Hospital of Lanzhou University, Lanzhou, 
      Gansu 730000, P.R. China.
FAU - Wang, Yuping
AU  - Wang Y
AD  - Division of Gastroenterology and Hepatology, The First Hospital of Lanzhou 
      University, Lanzhou, Gansu 730000, P.R. China.
FAU - Chen, Zhaofeng
AU  - Chen Z
AD  - Division of Gastroenterology and Hepatology, The First Hospital of Lanzhou 
      University, Lanzhou, Gansu 730000, P.R. China.
FAU - Ji, Rui
AU  - Ji R
AD  - Division of Gastroenterology and Hepatology, The First Hospital of Lanzhou 
      University, Lanzhou, Gansu 730000, P.R. China.
FAU - Guo, Qinghong
AU  - Guo Q
AD  - Division of Gastroenterology and Hepatology, The First Hospital of Lanzhou 
      University, Lanzhou, Gansu 730000, P.R. China.
FAU - Li, Qiang
AU  - Li Q
AD  - Division of Gastroenterology and Hepatology, The First Hospital of Lanzhou 
      University, Lanzhou, Gansu 730000, P.R. China.
FAU - Zhou, Yongning
AU  - Zhou Y
AD  - Division of Gastroenterology and Hepatology, The First Hospital of Lanzhou 
      University, Lanzhou, Gansu 730000, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20170602
PL  - Greece
TA  - Int J Mol Med
JT  - International journal of molecular medicine
JID - 9810955
RN  - 0 (NOTCH1 protein, human)
RN  - 0 (Receptor, Notch1)
RN  - 0 (Snail Family Transcription Factors)
RN  - EC 1.14.99.1 (Cyclooxygenase 2)
SB  - IM
MH  - Cell Line, Tumor
MH  - Cell Proliferation
MH  - Cyclooxygenase 2/*genetics
MH  - *Gene Expression Regulation, Neoplastic
MH  - Humans
MH  - Receptor, Notch1/*genetics
MH  - Signal Transduction
MH  - Snail Family Transcription Factors/*genetics
MH  - Stomach/pathology
MH  - Stomach Neoplasms/*genetics/pathology
EDAT- 2017/06/07 06:00
MHDA- 2018/05/31 06:00
CRDT- 2017/06/07 06:00
PHST- 2016/11/01 00:00 [received]
PHST- 2017/05/19 00:00 [accepted]
PHST- 2017/06/07 06:00 [pubmed]
PHST- 2018/05/31 06:00 [medline]
PHST- 2017/06/07 06:00 [entrez]
AID - 10.3892/ijmm.2017.3011 [doi]
PST - ppublish
SO  - Int J Mol Med. 2017 Aug;40(2):512-522. doi: 10.3892/ijmm.2017.3011. Epub 2017 Jun 
      2.