PMID- 28586101
OWN - NLM
STAT- MEDLINE
DCOM- 20170818
LR  - 20180209
IS  - 1873-3468 (Electronic)
IS  - 0014-5793 (Linking)
VI  - 591
IP  - 13
DP  - 2017 Jul
TI  - Functional role of glycosphingolipids in contact inhibition of growth in a human 
      mammary epithelial cell line.
PG  - 1918-1928
LID - 10.1002/1873-3468.12709 [doi]
AB  - We have demonstrated previously the involvement of certain glycosphingolipids 
      (GSLs) in 'contact inhibition' (dependent on cell-to-cell contact) of cell 
      growth. Here, we examined the roles of specific GSLs in contact inhibition of the 
      human epithelial cell line MCF10A. Contact-inhibited cells show increased 
      expression of the ganglioside GD3 and the globo-series GSL Gb3, and of the mRNAs 
      for the corresponding sialyltransferases ST8SIA1 (GD3 synthase) and 
      galactosyltransferase A4GALT (Gb3 synthase). siRNA knockdown (KD) of ST8SIA1 
      and/or A4GALT significantly suppresses contact inhibition. Exogenous addition of 
      GD3 or Gb3 inhibits proliferation of low-density cells. Our findings suggest that 
      GSLs play functional roles in contact inhibition of these cells and that 
      Merlin/NF2, a tumor suppressor protein, is involved in the GSL function.
CI  - (c) 2017 Federation of European Biochemical Societies.
FAU - Huang, Xiaohua
AU  - Huang X
AD  - Division of Biomembrane Research, Pacific Northwest Research Institute, Seattle, 
      WA, USA.
FAU - Schurman, Nathan
AU  - Schurman N
AD  - Division of Biomembrane Research, Pacific Northwest Research Institute, Seattle, 
      WA, USA.
FAU - Handa, Kazuko
AU  - Handa K
AD  - Division of Biomembrane Research, Pacific Northwest Research Institute, Seattle, 
      WA, USA.
FAU - Hakomori, Senitiroh
AU  - Hakomori S
AD  - Division of Biomembrane Research, Pacific Northwest Research Institute, Seattle, 
      WA, USA.
AD  - Department of Pathobiology and Global Health, University of Washington, Seattle, 
      WA, USA.
LA  - eng
PT  - Letter
PT  - Research Support, Non-U.S. Gov't
DEP - 20170622
PL  - England
TA  - FEBS Lett
JT  - FEBS letters
JID - 0155157
RN  - 0 (Glycosphingolipids)
RN  - 0 (RNA, Messenger)
RN  - 0 (RNA, Small Interfering)
RN  - EC 2.4.1.- (Galactosyltransferases)
RN  - EC 2.4.1.- (UDP-galactose-lactosylceramide alpha 1-4-galactosyltransferase)
RN  - EC 2.4.99.- (Sialyltransferases)
RN  - EC 2.4.99.8 (alpha-N-acetylneuraminate alpha-2,8-sialyltransferase)
SB  - IM
MH  - Cell Count
MH  - Cell Line, Tumor
MH  - Cell Proliferation
MH  - *Contact Inhibition
MH  - Galactosyltransferases/deficiency/genetics
MH  - Gene Expression Regulation
MH  - Gene Knockdown Techniques
MH  - Glycosphingolipids/*metabolism
MH  - Humans
MH  - Mammary Glands, Human/*cytology
MH  - RNA, Messenger/genetics/metabolism
MH  - RNA, Small Interfering/genetics
MH  - Sialyltransferases/deficiency/genetics
OTO - NOTNLM
OT  - Merlin/NF2
OT  - contact inhibition of cell growth
OT  - glycosphingolipids
OT  - human mammary epithelial cells
EDAT- 2017/06/07 06:00
MHDA- 2017/08/19 06:00
CRDT- 2017/06/07 06:00
PHST- 2017/04/06 00:00 [received]
PHST- 2017/05/22 00:00 [revised]
PHST- 2017/05/24 00:00 [accepted]
PHST- 2017/06/07 06:00 [pubmed]
PHST- 2017/08/19 06:00 [medline]
PHST- 2017/06/07 06:00 [entrez]
AID - 10.1002/1873-3468.12709 [doi]
PST - ppublish
SO  - FEBS Lett. 2017 Jul;591(13):1918-1928. doi: 10.1002/1873-3468.12709. Epub 2017 
      Jun 22.