PMID- 28586321
OWN - NLM
STAT- MEDLINE
DCOM- 20180319
LR  - 20190606
IS  - 1643-3750 (Electronic)
IS  - 1234-1010 (Print)
IS  - 1234-1010 (Linking)
VI  - 23
DP  - 2017 Jun 6
TI  - Treatment of Ischemia-Reperfusion Injury of the Skin Flap Using Human Umbilical 
      Cord Mesenchymal Stem Cells (hUC-MSCs) Transfected with "F-5" Gene.
PG  - 2751-2764
AB  - BACKGROUND Recent studies have shown that skin flap transplantation technique 
      plays an important role in surgical procedures. However, there are many problems 
      in the process of skin flap transplantation surgeries, especially 
      ischemia-reperfusion injury, which directly affects the survival rate of the skin 
      flap and patient prognosis after surgeries. MATERIAL AND METHODS In this study, 
      we used a new method of the "stem cells-gene" combination therapy. The "F-5" gene 
      fragment of heat shock protein 90-alpha (Hsp90-alpha) was transfected into human 
      umbilical cord mesenchymal stem cells (hUC-MSCs) by genetic engineering 
      technique. RESULTS The synergistic effects of "F-5" gene and hUC-MSCs in the 
      treatment of ischemia-reperfusion injury of the skin flap were confirmed by 
      histochemical and immunohistochemical methods. CONCLUSIONS This study showed that 
      the hUC-MSCs transfected with "F-5" gene can effectively improve the repair of 
      ischemia-reperfusion injury.
FAU - Leng, Xiangfeng
AU  - Leng X
AD  - Department of Plastic Surgery, Affiliated Hospital of Qingdao University, 
      Qingdao, Shandong, China (mainland).
FAU - Fan, Yongle
AU  - Fan Y
AD  - Department of Plastic Surgery, Affiliated Hospital of Qingdao University, 
      Qingdao, Shandong, China (mainland).
FAU - Wang, Yating
AU  - Wang Y
AD  - Department of Plastic Surgery, Affiliated Hospital of Qingdao University, 
      Qingdao, Shandong, China (mainland).
FAU - Sun, Jian
AU  - Sun J
AD  - Department of Nephrology, Qingdao Municipal Hospital, Qingdao, Shandong, China 
      (mainland).
FAU - Cai, Xia
AU  - Cai X
AD  - Department of Plastic Surgery, Affiliated Hospital of Qingdao University, 
      Qingdao, Shandong, China (mainland).
FAU - Hu, Chunnan
AU  - Hu C
AD  - Department of Plastic Surgery, Affiliated Hospital of Qingdao University, 
      Qingdao, Shandong, China (mainland).
FAU - Ding, Xiaoying
AU  - Ding X
AD  - Tianjin University, Tianjin, China (mainland).
FAU - Hu, Xiaoying
AU  - Hu X
AD  - The Eighth People's Hospital of Qingdao, Qingdao, Shandong, China (mainland).
FAU - Chen, Zhengyu
AU  - Chen Z
AD  - Department of Plastic Surgery, Affiliated Hospital of Qingdao University, 
      Qingdao, Shandong, China (mainland).
LA  - eng
PT  - Journal Article
DEP - 20170606
PL  - United States
TA  - Med Sci Monit
JT  - Medical science monitor : international medical journal of experimental and 
      clinical research
JID - 9609063
RN  - 0 (HSP90 Heat-Shock Proteins)
RN  - 0 (Platelet Endothelial Cell Adhesion Molecule-1)
SB  - IM
MH  - Animals
MH  - Disease Models, Animal
MH  - Fluorescence
MH  - HSP90 Heat-Shock Proteins/*genetics
MH  - Humans
MH  - Immunohistochemistry
MH  - Ischemia/*therapy
MH  - *Mesenchymal Stem Cell Transplantation
MH  - Mesenchymal Stem Cells/*cytology
MH  - Necrosis
MH  - Platelet Endothelial Cell Adhesion Molecule-1/metabolism
MH  - Rats, Wistar
MH  - Reperfusion Injury/*therapy
MH  - Skin/*blood supply/pathology
MH  - Surgical Flaps/*blood supply
MH  - Transfection
MH  - Umbilical Cord/*cytology
PMC - PMC5469319
COIS- Conflict of interest The authors have declared that there is no conflict of 
      interest in this study.
EDAT- 2017/06/07 06:00
MHDA- 2018/03/20 06:00
PMCR- 2017/06/06
CRDT- 2017/06/07 06:00
PHST- 2017/06/07 06:00 [entrez]
PHST- 2017/06/07 06:00 [pubmed]
PHST- 2018/03/20 06:00 [medline]
PHST- 2017/06/06 00:00 [pmc-release]
AID - 905216 [pii]
AID - 10.12659/msm.905216 [doi]
PST - epublish
SO  - Med Sci Monit. 2017 Jun 6;23:2751-2764. doi: 10.12659/msm.905216.