PMID- 28587087
OWN - NLM
STAT- MEDLINE
DCOM- 20180321
LR  - 20181113
IS  - 1660-3397 (Electronic)
IS  - 1660-3397 (Linking)
VI  - 15
IP  - 6
DP  - 2017 May 25
TI  - Taurine Biosynthesis in a Fish Liver Cell Line (ZFL) Adapted to a Serum-Free 
      Medium.
LID - 10.3390/md15060147 [doi]
LID - 147
AB  - Although taurine has been shown to play multiple important physiological roles in 
      teleosts, little is known about the molecular mechanisms underlying dietary 
      requirements. Cell lines can provide useful tools for deciphering biosynthetic 
      pathways and their regulation. However, culture media and sera contain variable 
      taurine levels. To provide a useful cell line for the investigation of taurine 
      homeostasis, an adult zebrafish liver cell line (ZFL) has been adapted to a 
      taurine-free medium by gradual accommodation to a commercially available 
      synthetic medium, UltraMEM-ITES. Here we show that ZFL cells are able to 
      synthesize taurine and be maintained in medium without taurine. This has allowed 
      for the investigation of the effects of taurine supplementation on cell growth, 
      cellular amino acid pools, as well as the expression of the taurine biosynthetic 
      pathway and taurine transporter genes in a defined fish cell type. After taurine 
      supplementation, cellular taurine levels increase but hypotaurine levels stay 
      constant, suggesting little suppression of taurine biosynthesis. Cellular 
      methionine levels do not change after taurine addition, consistent with 
      maintenance of taurine biosynthesis. The addition of taurine to cells grown in 
      taurine-free medium has little effect on transcript levels of the biosynthetic 
      pathway genes for cysteine dioxygenase (CDO), cysteine sulfinate decarboxylase 
      (CSAD), or cysteamine dioxygenase (ADO). In contrast, supplementation with 
      taurine causes a 30% reduction in transcript levels of the taurine transporter, 
      TauT. This experimental approach can be tailored for the development of cell 
      lines from aquaculture species for the elucidation of their taurine biosynthetic 
      capacity.
FAU - Liu, Chieh-Lun
AU  - Liu CL
AD  - Institute of Marine and Environmental Technology, University of Maryland Center 
      for Environmental Science, 701 E. Pratt Street, Baltimore, MD 21202, USA. 
      liuh@umces.edu.
FAU - Watson, Aaron M
AU  - Watson AM
AD  - Marine Resources Research Institute, South Carolina Department of Natural 
      Resources, 217 Fort Johnson Rd, Charleston, SC 29412, USA. WatsonA@dnr.sc.gov.
FAU - Place, Allen R
AU  - Place AR
AD  - Institute of Marine and Environmental Technology, University of Maryland Center 
      for Environmental Science, 701 E. Pratt Street, Baltimore, MD 21202, USA. 
      place@umces.edu.
FAU - Jagus, Rosemary
AU  - Jagus R
AD  - Institute of Marine and Environmental Technology, University of Maryland Center 
      for Environmental Science, 701 E. Pratt Street, Baltimore, MD 21202, USA. 
      jagus@umces.edu.
LA  - eng
PT  - Journal Article
DEP - 20170525
PL  - Switzerland
TA  - Mar Drugs
JT  - Marine drugs
JID - 101213729
RN  - 0 (Amino Acids)
RN  - 0 (Culture Media, Serum-Free)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (Membrane Transport Proteins)
RN  - 148686-53-7 (taurine transporter)
RN  - 1EQV5MLY3D (Taurine)
RN  - 5L08GE4332 (hypotaurine)
RN  - EC 1.13.11.- (Dioxygenases)
RN  - EC 1.13.11.19 (cysteamine dioxygenase)
RN  - EC 1.13.11.20 (Cysteine Dioxygenase)
RN  - EC 4.1.1.- (Carboxy-Lyases)
RN  - EC 4.1.1.29 (sulfoalanine decarboxylase)
SB  - IM
MH  - Amino Acids/metabolism
MH  - Animals
MH  - Carboxy-Lyases/metabolism
MH  - Cell Line
MH  - Culture Media, Serum-Free/*metabolism
MH  - Cysteine Dioxygenase/metabolism
MH  - Dioxygenases/metabolism
MH  - Liver/*metabolism
MH  - Membrane Glycoproteins/metabolism
MH  - Membrane Transport Proteins/metabolism
MH  - Taurine/analogs & derivatives/*biosynthesis/*metabolism
MH  - Zebrafish/*metabolism
PMC - PMC5484097
OTO - NOTNLM
OT  - ZFL cells
OT  - taurine biosynthesis
OT  - taurine-free medium
COIS- The authors declare no conflict of interest.
EDAT- 2017/06/08 06:00
MHDA- 2018/03/22 06:00
PMCR- 2017/06/01
CRDT- 2017/06/08 06:00
PHST- 2017/04/01 00:00 [received]
PHST- 2017/05/18 00:00 [revised]
PHST- 2017/05/22 00:00 [accepted]
PHST- 2017/06/08 06:00 [entrez]
PHST- 2017/06/08 06:00 [pubmed]
PHST- 2018/03/22 06:00 [medline]
PHST- 2017/06/01 00:00 [pmc-release]
AID - md15060147 [pii]
AID - marinedrugs-15-00147 [pii]
AID - 10.3390/md15060147 [doi]
PST - epublish
SO  - Mar Drugs. 2017 May 25;15(6):147. doi: 10.3390/md15060147.