PMID- 28587193 OWN - NLM STAT- MEDLINE DCOM- 20180326 LR - 20181113 IS - 1422-0067 (Electronic) IS - 1422-0067 (Linking) VI - 18 IP - 6 DP - 2017 Jun 3 TI - Stability of BDNF in Human Samples Stored Up to 6 Months and Correlations of Serum and EDTA-Plasma Concentrations. LID - 10.3390/ijms18061189 [doi] LID - 1189 AB - Brain-derived neurotrophic factor (BDNF), an important neural growth factor, has gained growing interest in neuroscience, but many influencing physiological and analytical aspects still remain unclear. In this study we assessed the impact of storage time at room temperature, repeated freeze/thaw cycles, and storage at -80 degrees C up to 6 months on serum and ethylenediaminetetraacetic acid (EDTA)-plasma BDNF. Furthermore, we assessed correlations of serum and plasma BDNF concentrations in two independent sets of samples. Coefficients of variations (CVs) for serum BDNF concentrations were significantly lower than CVs of plasma concentrations (n = 245, p = 0.006). Mean serum and plasma concentrations at all analyzed time points remained within the acceptable change limit of the inter-assay precision as declared by the manufacturer. Serum and plasma BDNF concentrations correlated positively in both sets of samples and at all analyzed time points of the stability assessment (r = 0.455 to r(s) = 0.596; p < 0.004). In summary, when considering the acceptable change limit, BDNF was stable in serum and in EDTA-plasma up to 6 months. Due to a higher reliability, we suggest favoring serum over EDTA-plasma for future experiments assessing peripheral BDNF concentrations. FAU - Polyakova, Maryna AU - Polyakova M AD - Department of Neurology, Max Planck Institute for Human Cognitive and Brain Sciences, 04103 Leipzig, Germany. polyakova@cbs.mpg.de. AD - Clinic for Psychiatry and Psychotherapy, University of Leipzig, 04103 Leipzig, Germany. polyakova@cbs.mpg.de. AD - LIFE-Leipzig Research Center for Civilization Diseases, University of Leipzig, 04103 Leipzig, Germany. polyakova@cbs.mpg.de. FAU - Schlogl, Haiko AU - Schlogl H AD - Department of Endocrinology, University Hospital Leipzig, 04103 Leipzig, Germany. haiko.schloegl@medizin.uni-leipzig.de. FAU - Sacher, Julia AU - Sacher J AD - Department of Neurology, Max Planck Institute for Human Cognitive and Brain Sciences, 04103 Leipzig, Germany. sacher@cbs.mpg.de. AD - LIFE-Leipzig Research Center for Civilization Diseases, University of Leipzig, 04103 Leipzig, Germany. sacher@cbs.mpg.de. FAU - Schmidt-Kassow, Maren AU - Schmidt-Kassow M AD - Institute of Medical Psychology, Goethe University Frankfurt, 60528 Frankfurt, Germany. schmidt-kassow@med.uni-frankfurt.de. FAU - Kaiser, Jochen AU - Kaiser J AD - Institute of Medical Psychology, Goethe University Frankfurt, 60528 Frankfurt, Germany. j.kaiser@med.uni-frankfurt.de. FAU - Stumvoll, Michael AU - Stumvoll M AD - Department of Endocrinology, University Hospital Leipzig, 04103 Leipzig, Germany. michael.stumvoll@medizin.uni-leipzig.de. FAU - Kratzsch, Jurgen AU - Kratzsch J AD - LIFE-Leipzig Research Center for Civilization Diseases, University of Leipzig, 04103 Leipzig, Germany. juergen.kratzsch@medizin.uni-leipzig.de. AD - Institute of Laboratory Medicine, Clinical Chemistry and Molecular Diagnostics, University Hospital Leipzig, 04103 Leipzig, Germany. juergen.kratzsch@medizin.uni-leipzig.de. FAU - Schroeter, Matthias L AU - Schroeter ML AD - Department of Neurology, Max Planck Institute for Human Cognitive and Brain Sciences, 04103 Leipzig, Germany. schroet@cbs.mpg.de. AD - LIFE-Leipzig Research Center for Civilization Diseases, University of Leipzig, 04103 Leipzig, Germany. schroet@cbs.mpg.de. AD - Clinic for Cognitive Neurology, University of Leipzig, 04103 Leipzig, Germany. schroet@cbs.mpg.de. LA - eng PT - Journal Article DEP - 20170603 PL - Switzerland TA - Int J Mol Sci JT - International journal of molecular sciences JID - 101092791 RN - 0 (Brain-Derived Neurotrophic Factor) SB - IM MH - *Blood Preservation MH - Brain-Derived Neurotrophic Factor/*blood MH - Cryopreservation MH - Enzyme-Linked Immunosorbent Assay MH - Humans MH - *Plasma MH - Protein Stability MH - Specimen Handling MH - Time Factors PMC - PMC5486012 OTO - NOTNLM OT - BDNF OT - EDTA-plasma OT - acceptable change limit OT - brain-derived neurotrophic factor OT - inter-assay precision OT - serum OT - stability COIS- The authors declare no conflict of interest. EDAT- 2017/06/08 06:00 MHDA- 2018/03/27 06:00 PMCR- 2017/06/01 CRDT- 2017/06/08 06:00 PHST- 2017/03/30 00:00 [received] PHST- 2017/05/10 00:00 [revised] PHST- 2017/05/30 00:00 [accepted] PHST- 2017/06/08 06:00 [entrez] PHST- 2017/06/08 06:00 [pubmed] PHST- 2018/03/27 06:00 [medline] PHST- 2017/06/01 00:00 [pmc-release] AID - ijms18061189 [pii] AID - ijms-18-01189 [pii] AID - 10.3390/ijms18061189 [doi] PST - epublish SO - Int J Mol Sci. 2017 Jun 3;18(6):1189. doi: 10.3390/ijms18061189.