PMID- 28587388
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200929
IS  - 1792-0981 (Print)
IS  - 1792-1015 (Electronic)
IS  - 1792-0981 (Linking)
VI  - 13
IP  - 6
DP  - 2017 Jun
TI  - Roles of the micro-opioid receptor and its related signaling pathways in the 
      pathogenesis of premenstrual syndrome liver-qi stagnation.
PG  - 3130-3136
LID - 10.3892/etm.2017.4374 [doi]
AB  - The present study aimed to investigate the roles of the micro-opioid receptor (MOR) 
      and its related signaling pathways in the pathogenesis of premenstrual syndrome 
      (PMS) liver-qi stagnation, along with the therapeutic effects of the Shu-Yu 
      capsule in treating the condition. A PMS liver-qi stagnation rat model was 
      established using a chronic restraint stress method. The protein expression level 
      of MOR within rat hippocampal tissue was detected via western blot analysis and 
      cyclic adenosine monophosphate (cAMP) levels within the supernatant of a rat 
      hippocampal cell culture were determined by ELISA. The western blot analysis 
      indicated that the hippocampal expression level of MOR was significantly elevated 
      in the PMS liver-qi stagnation model group. However, subsequent treatment with a 
      Shu-Yu capsule was found to significantly decrease the level of MOR expression. 
      In addition, in vitro experiments were performed, whereby primary hippocampal 
      neurons were treated with model rat serum. It was observed that the level of MOR 
      expression was significantly elevated, while brain-derived neurotrophic factor 
      (BDNF) and cAMP levels in the culture supernatant were significantly decreased. 
      These effects were reversed by treatment with serum from the Shu-Yu 
      capsule-treated rats. Furthermore, when treated with the MOR activator DAMGO, the 
      following were significantly decreased in the primary neurons: Phosphorylation 
      levels of cAMP response element binding protein and extracellular 
      signal-regulated protein kinases (ERK); BDNF expression; and cAMP content in the 
      culture supernatant. These effects were reversed in primary neurons treated with 
      DAMGO and Shu-Yu-containing rat serum. Collectively, the data suggest that 
      increased MOR expression and activation of the cAMP/ERK signaling pathway in the 
      hippocampus may be involved in the pathogenesis of PMS liver-qi stagnation. 
      Furthermore, the efficacy of the Shu-Yu capsule in treating the condition may be 
      via its regulation of MOR receptor signaling.
FAU - Song, Chunhong
AU  - Song C
AD  - Key Laboratory for Classical Theory of Traditional Chinese Medicine, The 
      Education Ministry, Shandong University of Traditional Chinese Medicine, Jinan, 
      Shandong 250355, P.R. China.
FAU - Xue, Ling
AU  - Xue L
AD  - Key Laboratory for Classical Theory of Traditional Chinese Medicine, The 
      Education Ministry, Shandong University of Traditional Chinese Medicine, Jinan, 
      Shandong 250355, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20170421
PL  - Greece
TA  - Exp Ther Med
JT  - Experimental and therapeutic medicine
JID - 101531947
PMC - PMC5450756
OTO - NOTNLM
OT  - Shu-Yu capsule
OT  - cyclic adenosine monophosphate/extracellular signal-regulated kinases signaling 
      pathway
OT  - liver-qi stagnation
OT  - premenstrual syndrome
OT  - micro-opioid receptor
EDAT- 2017/06/08 06:00
MHDA- 2017/06/08 06:01
PMCR- 2017/04/21
CRDT- 2017/06/08 06:00
PHST- 2015/12/28 00:00 [received]
PHST- 2016/12/23 00:00 [accepted]
PHST- 2017/06/08 06:00 [entrez]
PHST- 2017/06/08 06:00 [pubmed]
PHST- 2017/06/08 06:01 [medline]
PHST- 2017/04/21 00:00 [pmc-release]
AID - ETM-0-0-4374 [pii]
AID - 10.3892/etm.2017.4374 [doi]
PST - ppublish
SO  - Exp Ther Med. 2017 Jun;13(6):3130-3136. doi: 10.3892/etm.2017.4374. Epub 2017 Apr 
      21.