PMID- 28587571
OWN - NLM
STAT- MEDLINE
DCOM- 20181114
LR  - 20181114
IS  - 1933-7205 (Electronic)
IS  - 1933-7191 (Linking)
VI  - 25
IP  - 2
DP  - 2018 Feb
TI  - Expression Profiling of lncRNAs, miRNAs, and mRNAs and Their Differential 
      Expression in Leiomyoma Using Next-Generation RNA Sequencing.
PG  - 246-255
LID - 10.1177/1933719117711265 [doi]
AB  - The objective of this study was to identify the expression profile of long 
      noncoding RNAs (lncRNAs) with concurrent assessment of microRNA (miRNA) and 
      messenger RNA (mRNA) profiles in leiomyomas and paired myometrium using 
      next-generation RNA sequencing and assembly of RNA transcripts. Total RNA was 
      isolated from leiomyoma and paired myometrium (N = 8) and samples from 3 pairs 
      were subjected to RNA sequencing. Normalized assembly of over 48 000 lncRNAs 
      resulted in identification of 45 936 lncRNAs. Of these lncRNAs, 22 148 
      representing overlapping, intergenic, intronic, and antisense subtypes were 
      expressed in all paired tissues, with 5941 (2813 up- and 3128 downregulated at 
      >/=1.5 fold) differentially expressed in leiomyomas. Concurrent RNA sequencing 
      revealed the expression of 2588 miRNAs and 21 814 mRNAs, of which 392 miRNAs and 
      16 559 mRNAs were expressed in all paired tissues. Of these transcripts, 56 and 
      92 miRNAs and 2030 and 1825 mRNAs were up- or downregulated at >/=1.5 fold, 
      respectively, in leiomyoma as compared to myometrium. Using quantitative reverse 
      transcription-polymerase chain reaction (QRT-PCR), we confirmed the expression of 
      hepatocellular carcinoma upregulated (HULC), lnc-maternally expressed 3 (MEG3), 
      long intergenic ncRNA 890 (LINC00890), TSIX, long intergenic ncRNA 473 
      (LINC00473), lnc-KLF9-1, and lnc-POTEM-3 (lncRNA-ATB) in leiomyoma and matched 
      myometrium (N = 8). Collectively, the results presented here provide a 
      comprehensive expression profile of lncRNAs in leiomyomas with concurrent 
      integrated expression of miRNAs and mRNAs and implicate potential regulatory 
      functions of lncRNAs through interactions with specific miRNAs and mRNAs which 
      are known to be critical in the pathogenesis of leiomyoma.
FAU - Chuang, Tsai-Der
AU  - Chuang TD
AD  - 1 LA-Biomed Research Institute, Torrance, CA, USA.
AD  - 2 Department of Obstetrics and Gynecology, Harbor-UCLA Medical Center, Torrance, 
      CA, USA.
FAU - Khorram, Omid
AU  - Khorram O
AD  - 1 LA-Biomed Research Institute, Torrance, CA, USA.
AD  - 2 Department of Obstetrics and Gynecology, Harbor-UCLA Medical Center, Torrance, 
      CA, USA.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20170607
PL  - United States
TA  - Reprod Sci
JT  - Reproductive sciences (Thousand Oaks, Calif.)
JID - 101291249
RN  - 0 (MicroRNAs)
RN  - 0 (RNA, Long Noncoding)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Adult
MH  - Female
MH  - Gene Expression Profiling
MH  - Gene Expression Regulation
MH  - Gene Expression Regulation, Neoplastic
MH  - High-Throughput Nucleotide Sequencing
MH  - Humans
MH  - Leiomyoma/*genetics/metabolism/pathology
MH  - MicroRNAs/*genetics/metabolism
MH  - Middle Aged
MH  - Myometrium/metabolism/pathology
MH  - RNA, Long Noncoding/*genetics/metabolism
MH  - RNA, Messenger/metabolism
MH  - Uterine Neoplasms/*genetics/metabolism/pathology
OTO - NOTNLM
OT  - HULC
OT  - LINC00473
OT  - LINC00890
OT  - TSIX
OT  - leiomyoma
OT  - lnc-KLF9-1
OT  - lnc-MEG3
OT  - lnc-POTEM-3 (lncRNA-ATB)
OT  - lncRNA
OT  - next-generation RNA sequencing
EDAT- 2017/06/08 06:00
MHDA- 2018/11/15 06:00
CRDT- 2017/06/08 06:00
PHST- 2017/06/08 06:00 [pubmed]
PHST- 2018/11/15 06:00 [medline]
PHST- 2017/06/08 06:00 [entrez]
AID - 10.1177/1933719117711265 [doi]
PST - ppublish
SO  - Reprod Sci. 2018 Feb;25(2):246-255. doi: 10.1177/1933719117711265. Epub 2017 Jun 
      7.