PMID- 28588226
OWN - NLM
STAT- MEDLINE
DCOM- 20181218
LR  - 20181218
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 7
IP  - 1
DP  - 2017 Jun 6
TI  - The effects of Brazilian green propolis that contains flavonols against mutant 
      copper-zinc superoxide dismutase-mediated toxicity.
PG  - 2882
LID - 10.1038/s41598-017-03115-y [doi]
LID - 2882
AB  - Amyotrophic lateral sclerosis (ALS) is a fatal neurodegenerative disease 
      characterized by the selective and progressive loss of motor neurons. The purpose 
      of this study was to clarify effects of brazilian green propolis and the active 
      ingredient against ALS-associated mutant copper-zinc superoxide dismutase 
      (SOD1)-mediated toxicity. Ethanol extract of brazilian green propolis (EBGP) 
      protected N2a cells against mutant SOD1-induced neurotoxicity and reduced 
      aggregated mutant SOD1 by induction of autophagy. Kaempferide and kaempferol, the 
      active ingredients of EBGP, also inhibited mutant SOD1-induced cell death and 
      reduced the intracellular mutant SOD1 aggregates. Both kaempferide and kaempferol 
      significantly suppressed mutant SOD1-induced superoxide in mitochondria. Western 
      blot analysis showed that kaempferol potentially induced autophagy via the 
      AMP-activated protein kinase (AMPK) - the mammalian target of rapamycin (mTOR) 
      pathway. These results suggest that EBGP containing the active ingredient against 
      mutant SOD1-mediated toxicity is a promising medicine or health food for 
      prevention and treatment of ALS.
FAU - Ueda, Tomoyuki
AU  - Ueda T
AD  - Lab. Medical Therapeutics and Molecular Therapeutics, Gifu Pharmaceutical Univ, 
      Gifu, Japan.
FAU - Inden, Masatoshi
AU  - Inden M
AD  - Lab. Medical Therapeutics and Molecular Therapeutics, Gifu Pharmaceutical Univ, 
      Gifu, Japan.
FAU - Shirai, Katsuhiro
AU  - Shirai K
AD  - Lab. Medical Therapeutics and Molecular Therapeutics, Gifu Pharmaceutical Univ, 
      Gifu, Japan.
FAU - Sekine, Shin-Ichiro
AU  - Sekine SI
AD  - Lab. Medical Therapeutics and Molecular Therapeutics, Gifu Pharmaceutical Univ, 
      Gifu, Japan.
FAU - Masaki, Yuji
AU  - Masaki Y
AD  - Lab. Medical Therapeutics and Molecular Therapeutics, Gifu Pharmaceutical Univ, 
      Gifu, Japan.
FAU - Kurita, Hisaka
AU  - Kurita H
AD  - Lab. Medical Therapeutics and Molecular Therapeutics, Gifu Pharmaceutical Univ, 
      Gifu, Japan.
FAU - Ichihara, Kenji
AU  - Ichihara K
AD  - Nagaragawa Research Center, Api Company Limited, Gifu, Japan.
FAU - Inuzuka, Takashi
AU  - Inuzuka T
AD  - Department of Neurology and Geriatrics, Gifu University Graduate School of 
      Medicine, Gifu, Japan.
FAU - Hozumi, Isao
AU  - Hozumi I
AD  - Lab. Medical Therapeutics and Molecular Therapeutics, Gifu Pharmaceutical Univ, 
      Gifu, Japan. hozumi@gifu-pu.ac.jp.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20170606
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - 0 (Antioxidants)
RN  - 0 (Flavonols)
RN  - 0 (Kaempferols)
RN  - 0 (Protective Agents)
RN  - 0 (Protein Aggregates)
RN  - 731P2LE49E (kaempferol)
RN  - 9009-62-5 (Propolis)
RN  - EC 1.15.1.1 (Superoxide Dismutase-1)
RN  - EC 2.7.11.31 (AMP-Activated Protein Kinases)
SB  - IM
MH  - AMP-Activated Protein Kinases/metabolism
MH  - Animals
MH  - Antioxidants/chemistry/pharmacology
MH  - Autophagy
MH  - Flavonols/chemistry/*pharmacology
MH  - Kaempferols/pharmacology
MH  - Mice
MH  - Motor Neurons/drug effects/metabolism
MH  - *Mutation
MH  - Phosphorylation
MH  - Propolis/chemistry/*pharmacology
MH  - Protective Agents/chemistry/*pharmacology
MH  - Protein Aggregates
MH  - Superoxide Dismutase-1/*genetics/*metabolism
PMC - PMC5460160
COIS- The authors declare that they have no competing interests.
EDAT- 2017/06/08 06:00
MHDA- 2018/12/19 06:00
PMCR- 2017/06/06
CRDT- 2017/06/08 06:00
PHST- 2016/12/21 00:00 [received]
PHST- 2017/04/24 00:00 [accepted]
PHST- 2017/06/08 06:00 [entrez]
PHST- 2017/06/08 06:00 [pubmed]
PHST- 2018/12/19 06:00 [medline]
PHST- 2017/06/06 00:00 [pmc-release]
AID - 10.1038/s41598-017-03115-y [pii]
AID - 3115 [pii]
AID - 10.1038/s41598-017-03115-y [doi]
PST - epublish
SO  - Sci Rep. 2017 Jun 6;7(1):2882. doi: 10.1038/s41598-017-03115-y.