PMID- 2859017
OWN - NLM
STAT- MEDLINE
DCOM- 19850501
LR  - 20190612
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 127
IP  - 3
DP  - 1985 Mar 29
TI  - GRF is a highly potent activator of adenylate cyclase in the normal human, bovine 
      and rat pituitary: interaction with somatostatin.
PG  - 977-85
AB  - The effect of GRF adenylate cyclase activation was studied in normal human, 
      bovine and rat pituitary tissues. Human GRF (hGRF) activates adenylate cyclase in 
      normal human pituitary membrane preparations in a concentration dependent manner 
      (ED5 0 = 10(-11) M). In bovine pituitary cells hGRF stimulates GH secretion into 
      the medium (ED5 0 = 7 X 10(-12) M) and activates adenylate cyclase (ED5 0 = 
      10(-11) M). In normal rat pituitary cells in monolayer culture, rat GRF (rGRF) 
      stimulates adenylate cyclase (ED5 0 = 3 X 10(-11) M). In normal human pituitary 
      membrane preparations and in normal rat pituitary cells in culture, somatostatin 
      inhibits GRF-stimulated adenylate cyclase in a non-competitive manner, while it 
      does not affect basal (i.e. non-stimulated) adenylate cyclase levels. VIP, a 
      peptide which is structurally homologous to hGRF and rGRF is a weak GRF-agonist 
      and activates adenylate cyclase in human and rat pituitary preparations at 
      concentrations greater than 10 nM.
FAU - Reyl-Desmars, F
AU  - Reyl-Desmars F
FAU - Baird, A
AU  - Baird A
FAU - Zeytin, F N
AU  - Zeytin FN
LA  - eng
GR  - AM-18811/AM/NIADDK NIH HHS/United States
GR  - HD-09690/HD/NICHD NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Adenylyl Cyclase Inhibitors)
RN  - 37221-79-7 (Vasoactive Intestinal Peptide)
RN  - 51110-01-1 (Somatostatin)
RN  - 9002-72-6 (Growth Hormone)
RN  - 9034-39-3 (Growth Hormone-Releasing Hormone)
RN  - EC 4.6.1.1 (Adenylyl Cyclases)
SB  - IM
MH  - Adenylyl Cyclase Inhibitors
MH  - Adenylyl Cyclases/*metabolism
MH  - Animals
MH  - Cattle
MH  - Cells, Cultured
MH  - Enzyme Activation/drug effects
MH  - Growth Hormone/metabolism
MH  - Growth Hormone-Releasing Hormone/*pharmacology
MH  - Humans
MH  - Pituitary Gland/drug effects/*enzymology
MH  - Rats
MH  - Somatostatin/*pharmacology
MH  - Species Specificity
MH  - Vasoactive Intestinal Peptide/pharmacology
EDAT- 1985/03/29 00:00
MHDA- 1985/03/29 00:01
CRDT- 1985/03/29 00:00
PHST- 1985/03/29 00:00 [pubmed]
PHST- 1985/03/29 00:01 [medline]
PHST- 1985/03/29 00:00 [entrez]
AID - S0006-291X(85)80040-1 [pii]
AID - 10.1016/s0006-291x(85)80040-1 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 1985 Mar 29;127(3):977-85. doi: 
      10.1016/s0006-291x(85)80040-1.