PMID- 28591312
OWN - NLM
STAT- MEDLINE
DCOM- 20170824
LR  - 20181113
IS  - 1678-8060 (Electronic)
IS  - 0074-0276 (Print)
IS  - 0074-0276 (Linking)
VI  - 112
IP  - 7
DP  - 2017 Jul
TI  - Identification of a type I nitroreductase gene in non-virulent Trypanosoma 
      rangeli.
PG  - 504-509
LID - S0074-02762017000700504 [pii]
LID - 10.1590/0074-02760160532 [doi]
AB  - Trypanosomatid type I nitroreductases (NTRs), i.e., mitochondrial enzymes that 
      metabolise nitroaromatic pro-drugs, are essential for parasite growth, infection, 
      and survival. Here, a type I NTR of non-virulent protozoan Trypanosoma rangeli is 
      described and compared to those of other trypanosomatids. The NTR gene was 
      isolated from KP1(+) and KP1(-) strains, and its corresponding transcript and 5' 
      untranslated region (5'UTR) were determined. Bioinformatics analyses and 
      nitro-drug activation assays were also performed. The results indicated that the 
      type I NTR gene is present in both KP1(-) and KP1(+) strains, with 98% identity. 
      However, the predicted subcellular localisation of the protein differed among the 
      strains (predicted as mitochondrial in the KP1(+) strain). Comparisons of the 
      domains and 3D structures of the NTRs with those of orthologs demonstrated that 
      the nitroreductase domain of T. rangeli NTR is conserved across all the strains, 
      including the residues involved in the interaction with the FMN cofactor and in 
      the tertiary structure characteristics of this oxidoreductase protein family. 
      mRNA processing and expression were also observed. In addition, T. rangeli was 
      shown to be sensitive to benznidazole and nifurtimox in a concentration-dependent 
      manner. In summary, T. rangeli appears to have a newly discovered functional type 
      I NTR.
FAU - Montenegro, Marjorie
AU  - Montenegro M
AD  - Pontificia Universidad Javeriana, Facultad de Ciencias, Departamento de 
      Microbiologia, Laboratorio de Parasitologia Molecular, Bogota, Colombia.
AD  - Consejo Superior de Investigaciones Cientificas, Instituto de Parasitologia y 
      Biomedicina Lopez Neyra, Granada, Spain.
FAU - Cuervo, Claudia
AU  - Cuervo C
AD  - Pontificia Universidad Javeriana, Facultad de Ciencias, Departamento de 
      Microbiologia, Laboratorio de Parasitologia Molecular, Bogota, Colombia.
FAU - Cardenas, Constanza
AU  - Cardenas C
AD  - Pontificia Universidad Catolica de Valparaiso, Nucleo de Biotecnologia Curauma, 
      Valparaiso, Chile.
FAU - Duarte, Silvia
AU  - Duarte S
AD  - Pontificia Universidad Javeriana, Facultad de Ciencias, Departamento de 
      Microbiologia, Laboratorio de Parasitologia Molecular, Bogota, Colombia.
FAU - Diaz, Jenny R
AU  - Diaz JR
AD  - Pontificia Universidad Javeriana, Facultad de Ciencias, Departamento de 
      Microbiologia, Laboratorio de Parasitologia Molecular, Bogota, Colombia.
FAU - Thomas, M Carmen
AU  - Thomas MC
AD  - Consejo Superior de Investigaciones Cientificas, Instituto de Parasitologia y 
      Biomedicina Lopez Neyra, Granada, Spain.
FAU - Lopez, Manuel C
AU  - Lopez MC
AD  - Consejo Superior de Investigaciones Cientificas, Instituto de Parasitologia y 
      Biomedicina Lopez Neyra, Granada, Spain.
FAU - Puerta, Concepcion J
AU  - Puerta CJ
AD  - Pontificia Universidad Javeriana, Facultad de Ciencias, Departamento de 
      Microbiologia, Laboratorio de Parasitologia Molecular, Bogota, Colombia.
LA  - eng
PT  - Journal Article
PL  - Brazil
TA  - Mem Inst Oswaldo Cruz
JT  - Memorias do Instituto Oswaldo Cruz
JID - 7502619
RN  - 0 (DNA, Protozoan)
RN  - EC 1.- (aromatic NADH-dependent nitroreductase)
RN  - EC 1.7.- (Nitroreductases)
SB  - IM
MH  - Base Sequence
MH  - DNA, Protozoan/genetics
MH  - Genetic Variation/genetics
MH  - Humans
MH  - Nitroreductases/*genetics
MH  - Sequence Analysis, DNA
MH  - Trypanosoma rangeli/*enzymology/genetics
PMC - PMC5452488
EDAT- 2017/06/08 06:00
MHDA- 2017/08/25 06:00
PMCR- 2017/07/01
CRDT- 2017/06/08 06:00
PHST- 2016/12/12 00:00 [received]
PHST- 2017/03/22 00:00 [accepted]
PHST- 2017/07/01 00:00 [pmc-release]
PHST- 2017/06/08 06:00 [entrez]
PHST- 2017/06/08 06:00 [pubmed]
PHST- 2017/08/25 06:00 [medline]
AID - S0074-02762017000700504 [pii]
AID - 0074-02760160532 [pii]
AID - 10.1590/0074-02760160532 [doi]
PST - ppublish
SO  - Mem Inst Oswaldo Cruz. 2017 Jul;112(7):504-509. doi: 10.1590/0074-02760160532.