PMID- 28595084
OWN - NLM
STAT- MEDLINE
DCOM- 20180511
LR  - 20181202
IS  - 1532-2661 (Electronic)
IS  - 0034-5288 (Linking)
VI  - 115
DP  - 2017 Dec
TI  - Identification of peripheral blood involvement in dogs with large B-cell 
      lymphoma: Comparison of different methods.
PG  - 288-293
LID - S0034-5288(17)30031-0 [pii]
LID - 10.1016/j.rvsc.2017.05.027 [doi]
AB  - Stage V lymphoma is defined as the presence of neoplastic cells in peripheral 
      blood (PB), bone marrow, or any other non-lymphoid tissue. Still, official 
      guidelines do not specify which technique should be used to assess infiltration. 
      We assessed the agreement among flow cytometry (FC), blood smear evaluation, and 
      ADVIA120 (LUC and BASO) to quantify PB infiltration in 100 dogs with large B-cell 
      lymphoma (LBCL). Significant errors were found for all methods compared to FC. A 
      moderate agreement was present between FC and blood smear evaluation, whereas LUC 
      and BASO had excellent specificity but unsatisfactory sensitivity in detecting FC 
      infiltrated PB samples. The different techniques should not be used 
      alternatively. We support the use of LUC/BASO as a speedy preliminary test to 
      detect infiltrated samples, and the joined use of blood smear evaluation and FC 
      to quantify definitively the infiltration. Our results are valid only within 
      canine LBCL staging workup, once the diagnosis has been confirmed.
CI  - Copyright (c) 2017 Elsevier Ltd. All rights reserved.
FAU - Riondato, Fulvio
AU  - Riondato F
AD  - Department of Veterinary Sciences, University of Turin, Largo Braccini 2, 
      Grugliasco, Turin, Italy. Electronic address: fulvio.riondato@unito.it.
FAU - Martini, Valeria
AU  - Martini V
AD  - Department of Veterinary Medicine, University of Milan, via Celoria 10, Milan, 
      Italy.
FAU - Poggi, Alessia
AU  - Poggi A
AD  - Department of Veterinary Sciences, University of Turin, Largo Braccini 2, 
      Grugliasco, Turin, Italy.
FAU - Massaglia, Ilaria
AU  - Massaglia I
AD  - Department of Veterinary Sciences, University of Turin, Largo Braccini 2, 
      Grugliasco, Turin, Italy.
FAU - Comazzi, Stefano
AU  - Comazzi S
AD  - Department of Veterinary Medicine, University of Milan, via Celoria 10, Milan, 
      Italy.
FAU - Borrelli, Antonio
AU  - Borrelli A
AD  - Department of Veterinary Sciences, University of Turin, Largo Braccini 2, 
      Grugliasco, Turin, Italy.
FAU - Miniscalco, Barbara
AU  - Miniscalco B
AD  - Department of Veterinary Sciences, University of Turin, Largo Braccini 2, 
      Grugliasco, Turin, Italy.
LA  - eng
PT  - Comparative Study
PT  - Journal Article
DEP - 20170531
PL  - England
TA  - Res Vet Sci
JT  - Research in veterinary science
JID - 0401300
SB  - IM
MH  - Animals
MH  - Blood Specimen Collection/methods/*veterinary
MH  - Diagnostic Tests, Routine/methods/*veterinary
MH  - Dog Diseases/*diagnosis
MH  - Dogs
MH  - Flow Cytometry/methods/*veterinary
MH  - Hematologic Tests/methods/*veterinary
MH  - Lymphoma, B-Cell/diagnosis/*veterinary
MH  - Sensitivity and Specificity
OTO - NOTNLM
OT  - ADVIA 120
OT  - Blood smear evaluation
OT  - Flow cytometry
OT  - Infiltration
OT  - Lymphoma
OT  - Staging
EDAT- 2017/06/09 06:00
MHDA- 2018/05/12 06:00
CRDT- 2017/06/09 06:00
PHST- 2017/01/11 00:00 [received]
PHST- 2017/05/22 00:00 [revised]
PHST- 2017/05/31 00:00 [accepted]
PHST- 2017/06/09 06:00 [pubmed]
PHST- 2018/05/12 06:00 [medline]
PHST- 2017/06/09 06:00 [entrez]
AID - S0034-5288(17)30031-0 [pii]
AID - 10.1016/j.rvsc.2017.05.027 [doi]
PST - ppublish
SO  - Res Vet Sci. 2017 Dec;115:288-293. doi: 10.1016/j.rvsc.2017.05.027. Epub 2017 May 
      31.