PMID- 2859538
OWN - NLM
STAT- MEDLINE
DCOM- 19850606
LR  - 20181130
IS  - 0028-3835 (Print)
IS  - 0028-3835 (Linking)
VI  - 40
IP  - 3
DP  - 1985 Mar
TI  - Somatostatin release from the median eminence of unanesthetized rats: lack of 
      correlation with pharmacologically suppressed growth hormone secretion.
PG  - 193-200
AB  - We describe a push-pull perfusion technique to investigate the role of 
      somatostatin in the pharmacological suppression of growth hormone (GH) secretion. 
      Immunoreactive somatostatin (IRS) released from the median eminence (ME) was 
      studied in chronically cannulated, unanesthetized male rats. In control rats 
      which received vehicle injection, plasma GH levels showed a normal ultradian 
      rhythm and relatively stable levels of IRS (around 30 pg/15 min) appeared in the 
      perfusate during the 6-h perfusion. Intracerebroventricular (i.c.v) 
      administration of human growth hormone (40 micrograms), neurotensin (2 
      micrograms), glucagon (25 micrograms) or intravenous (i.v.) injection of 
      oxymetazoline (50 micrograms/kg b.w.), an alpha-adrenergic agonist, or endotoxin 
      (150 micrograms/kg b.w.) suppressed subsequent GH surges. In these rats, however, 
      IRS levels in the ME perfusate failed to change significantly compared to control 
      rats. These results suggest that changes in somatostatin release may play a minor 
      role in the suppression of plasma GH levels caused by these substances, and that 
      major regulatory effects may be achieved via the suppression of growth 
      hormone-releasing factor release.
FAU - Fukata, J
AU  - Fukata J
FAU - Kasting, N W
AU  - Kasting NW
FAU - Martin, J B
AU  - Martin JB
LA  - eng
GR  - AM26252/AM/NIADDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Switzerland
TA  - Neuroendocrinology
JT  - Neuroendocrinology
JID - 0035665
RN  - 0 (Lipopolysaccharides)
RN  - 39379-15-2 (Neurotensin)
RN  - 51110-01-1 (Somatostatin)
RN  - 8VLN5B44ZY (Oxymetazoline)
RN  - 9002-72-6 (Growth Hormone)
RN  - 9007-92-5 (Glucagon)
SB  - IM
MH  - Animals
MH  - Escherichia coli
MH  - Glucagon/pharmacology
MH  - Growth Hormone/*metabolism/pharmacology
MH  - Lipopolysaccharides/pharmacology
MH  - Male
MH  - Median Eminence/*metabolism
MH  - Neurotensin/pharmacology
MH  - Oxymetazoline/pharmacology
MH  - Pituitary Gland, Anterior/*metabolism
MH  - Rats
MH  - Somatostatin/*metabolism
EDAT- 1985/03/01 00:00
MHDA- 1985/03/01 00:01
CRDT- 1985/03/01 00:00
PHST- 1985/03/01 00:00 [pubmed]
PHST- 1985/03/01 00:01 [medline]
PHST- 1985/03/01 00:00 [entrez]
AID - 10.1159/000124074 [doi]
PST - ppublish
SO  - Neuroendocrinology. 1985 Mar;40(3):193-200. doi: 10.1159/000124074.