PMID- 28595728
OWN - NLM
STAT- MEDLINE
DCOM- 20180614
LR  - 20181202
IS  - 1873-4324 (Electronic)
IS  - 0003-2670 (Linking)
VI  - 978
DP  - 2017 Jul 25
TI  - On-line double focusing of atenolol and metoprolol in human urine using capillary 
      electrophoresis with the aid of beta-cyclodextrin.
PG  - 61-67
LID - S0003-2670(17)30577-9 [pii]
LID - 10.1016/j.aca.2017.04.052 [doi]
AB  - A maneuverable and sensitive on-line double focusing technique combined field 
      amplified sample stacking (FASS) with micelle to solvent stacking (MSS) with the 
      aid of beta-cyelodextrin (beta-CD) is developed to detect the contents of AT and ME in 
      human urine by capillary electrophoresis (CE) with UV detector. Small amount of 
      beta-CD not only increase the critical micelle concentration (CMC) of SDS, but also 
      strengthen the interaction between SDS and the aimed compound by forming 
      inclusion complexes. The result indicates that the addition of beta-CD affords 5.5- 
      and 3.5-fold improvements for atenolol (AT) and metoprolol (ME) in sensitivity 
      than that of in the absence of beta-CD in the double focusing system, respectively. 
      Under the optimal conditions, about 200-fold improvement in sensitivity for 
      analytes is achieved compared with conventional CE method. The limits of 
      detection (LODs) at a signal-to-noise of 3 (S/N = 3) of the two beta-blockers can be 
      reached 3.3 and 3.7 ng mL(-1) respectively, which are lower than minimum required 
      performance levels (MRPLs) set by the World Anti Doping Agency. The relative 
      standard deviations (RSDs) of peak areas of intra-day and inter-day are 
      3.51-3.38% and 2.34-4.28% (n = 6), respectively. AT and ME in urine without 
      special pretreatment and additional instrument are analyzed. The recoveries are 
      82-98% with RSDs less than 2.0%.
CI  - Copyright (c) 2017 Elsevier B.V. All rights reserved.
FAU - Wan, Qian
AU  - Wan Q
AD  - Guangdong Provincial Key Laboratory of New Drug Screening, School of 
      Pharmaceutical Sciences, Southern Medical University, Guangzhou, 510515, China.
FAU - Liu, Yanhui
AU  - Liu Y
AD  - Internal Medicine Ward, Jinzhou People's Hospital, 1 Chaoyang Street, Jinzhou 
      052260, China.
FAU - Yang, Chunli
AU  - Yang C
AD  - Laboratory Medicine Center, Nanfang Hospital, Southern Medical University, 
      Guangzhou, Guangdong 510515, China.
FAU - Liu, Lihong
AU  - Liu L
AD  - Guangdong Provincial Key Laboratory of New Drug Screening, School of 
      Pharmaceutical Sciences, Southern Medical University, Guangzhou, 510515, China. 
      Electronic address: lhliu02@126.com.
LA  - eng
PT  - Journal Article
DEP - 20170513
PL  - Netherlands
TA  - Anal Chim Acta
JT  - Analytica chimica acta
JID - 0370534
RN  - 0 (Adrenergic beta-Antagonists)
RN  - 0 (beta-Cyclodextrins)
RN  - 50VV3VW0TI (Atenolol)
RN  - GEB06NHM23 (Metoprolol)
SB  - IM
MH  - Adrenergic beta-Antagonists/urine
MH  - Atenolol/*urine
MH  - *Electrophoresis, Capillary
MH  - Humans
MH  - Metoprolol/*urine
MH  - *beta-Cyclodextrins
OTO - NOTNLM
OT  - Atenolol
OT  - Capillary electrophoresis
OT  - Field amplified sample stacking
OT  - Metoprolol
OT  - Micelle to solvent stacking
OT  - beta-cyelodextrin
EDAT- 2017/06/10 06:00
MHDA- 2018/06/15 06:00
CRDT- 2017/06/10 06:00
PHST- 2017/01/03 00:00 [received]
PHST- 2017/04/06 00:00 [revised]
PHST- 2017/04/27 00:00 [accepted]
PHST- 2017/06/10 06:00 [entrez]
PHST- 2017/06/10 06:00 [pubmed]
PHST- 2018/06/15 06:00 [medline]
AID - S0003-2670(17)30577-9 [pii]
AID - 10.1016/j.aca.2017.04.052 [doi]
PST - ppublish
SO  - Anal Chim Acta. 2017 Jul 25;978:61-67. doi: 10.1016/j.aca.2017.04.052. Epub 2017 
      May 13.