PMID- 28597195
OWN - NLM
STAT- MEDLINE
DCOM- 20180126
LR  - 20181113
IS  - 1572-994X (Electronic)
IS  - 0920-8569 (Linking)
VI  - 53
IP  - 4
DP  - 2017 Aug
TI  - Identification of epitopes on nonstructural protein 7 of porcine reproductive and 
      respiratory syndrome virus recognized by monoclonal antibodies using 
      phage-display technology.
PG  - 623-635
LID - 10.1007/s11262-017-1472-9 [doi]
AB  - Nonstructural protein 7 (nsp7) of porcine reproductive and respiratory syndrome 
      virus (PRRSV) is considered to be a suitable reagent for the development of 
      serological diagnostic assays. It can be expressed as a soluble recombinant 
      protein in Escherichia coli, and its antibody response may continue up to 
      202 days post-infection. Furthermore, the region encoded by nsp7 is highly 
      homologous among various strains within the genotype, and the results of 
      nsp7-based enzyme-linked immunosorbent assay (ELISA) showed high agreement with 
      previous Idexx ELISA results. All these evidences suggest the existence of 
      important epitopes on nsp7, though the characteristics of these epitopes remain 
      unclear. In the present study, we prepared three monoclonal antibodies against 
      nsp7 protein and used them to screen the epitope-distribution characteristics of 
      PRRSV nsp7 protein by phage-display technology. We identified a linear epitope 
      NAWGDEDRLN at amino acids 153-162 type II PRRSV nsp7beta subunit. This newly defined 
      epitope showed excellent reactivity with PRSSV-positive serum samples. These 
      results further our understanding of the antigenic structure of nsp7 protein, and 
      provide efficient reagents for PRRSV serological tests.
FAU - Wang, Heng
AU  - Wang H
AD  - College of Veterinary Medicine, South China Agricultural University, 483 Wushan 
      Road, Tianhe District, Guangzhou, 510642, Guangdong Province, China.
AD  - Guangdong Provincial Key Laboratory of Prevention and Control for Severe Clinical 
      Animal Diseases, Guangzhou, 510642, Guangdong Province, China.
FAU - Liu, Rongchang
AU  - Liu R
AD  - Institute of Animal Husbandry and Veterinary Medicine, Fujian Academy of 
      Agricultural Sciences, Fuzhou, 350013, Fujian Province, China.
FAU - Zhang, Weidong
AU  - Zhang W
AD  - Hospital of South China Agricultural University, Guangzhou, 510642, Guangdong 
      Province, China.
FAU - Sun, Lingshuang
AU  - Sun L
AD  - College of Veterinary Medicine, South China Agricultural University, 483 Wushan 
      Road, Tianhe District, Guangzhou, 510642, Guangdong Province, China.
AD  - Guangdong Provincial Key Laboratory of Prevention and Control for Severe Clinical 
      Animal Diseases, Guangzhou, 510642, Guangdong Province, China.
FAU - Ning, Zhangyong
AU  - Ning Z
AD  - College of Veterinary Medicine, South China Agricultural University, 483 Wushan 
      Road, Tianhe District, Guangzhou, 510642, Guangdong Province, China.
AD  - Guangdong Provincial Key Laboratory of Prevention and Control for Severe Clinical 
      Animal Diseases, Guangzhou, 510642, Guangdong Province, China.
FAU - Ji, Fangxiao
AU  - Ji F
AD  - MOA Key Laboratory of Animal Vaccine Development, Guangzhou, 510642, Guangdong 
      Province, China.
FAU - Cui, Jin
AU  - Cui J
AD  - MOA Key Laboratory of Animal Vaccine Development, Guangzhou, 510642, Guangdong 
      Province, China.
FAU - Zhang, Guihong
AU  - Zhang G
AD  - College of Veterinary Medicine, South China Agricultural University, 483 Wushan 
      Road, Tianhe District, Guangzhou, 510642, Guangdong Province, China. 
      guihongzh@scau.edu.cn.
AD  - MOA Key Laboratory of Animal Vaccine Development, Guangzhou, 510642, Guangdong 
      Province, China. guihongzh@scau.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20170608
PL  - United States
TA  - Virus Genes
JT  - Virus genes
JID - 8803967
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Antibodies, Viral)
RN  - 0 (Epitopes)
RN  - 0 (Viral Nonstructural Proteins)
SB  - IM
MH  - Amino Acid Motifs
MH  - Animals
MH  - Antibodies, Monoclonal/immunology
MH  - Antibodies, Viral/immunology
MH  - Bacteriophages/genetics/metabolism
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Epitopes/chemistry/genetics/*immunology
MH  - Porcine Reproductive and Respiratory Syndrome/immunology/*virology
MH  - Porcine respiratory and reproductive syndrome 
      virus/chemistry/genetics/*immunology
MH  - Swine
MH  - Viral Nonstructural Proteins/*chemistry/genetics/*immunology
OTO - NOTNLM
OT  - Antigenic structure
OT  - Epitopes
OT  - Nonstructural protein 7
OT  - Phage-display technology
OT  - Porcine reproductive and respiratory syndrome virus
EDAT- 2017/06/10 06:00
MHDA- 2018/01/27 06:00
CRDT- 2017/06/10 06:00
PHST- 2016/04/26 00:00 [received]
PHST- 2017/06/02 00:00 [accepted]
PHST- 2017/06/10 06:00 [pubmed]
PHST- 2018/01/27 06:00 [medline]
PHST- 2017/06/10 06:00 [entrez]
AID - 10.1007/s11262-017-1472-9 [pii]
AID - 10.1007/s11262-017-1472-9 [doi]
PST - ppublish
SO  - Virus Genes. 2017 Aug;53(4):623-635. doi: 10.1007/s11262-017-1472-9. Epub 2017 
      Jun 8.