PMID- 28597993
OWN - NLM
STAT- MEDLINE
DCOM- 20180105
LR  - 20180105
IS  - 1099-1565 (Electronic)
IS  - 0958-0344 (Linking)
VI  - 28
IP  - 6
DP  - 2017 Nov
TI  - One-Step Extraction and Hydrolysis of Flavonoid Glycosides in Rape Bee Pollen 
      Based on Soxhlet-Assisted Matrix Solid Phase Dispersion.
PG  - 505-511
LID - 10.1002/pca.2699 [doi]
AB  - INTRODUCTION: Flavonoids are frequently found as glycosylated derivatives in 
      plant materials. To determine contents of flavonoid aglycones in these matrices, 
      procedures for the extraction and hydrolysis of flavonoid glycosides are 
      required. The current sample preparation method is both labour and time 
      consuming. OBJECTIVE: Develop a modified matrix solid phase dispersion (MSPD) 
      procedure as an alternative methodology for the one-step extraction and 
      hydrolysis of flavonoid glycosides. METHOD: HPLC-DAD was applied for 
      demonstrating the one-step extraction and hydrolysis of flavonoids in rape bee 
      pollen. The obtained contents of flavonoid aglycones (quercetin, kaempferol, 
      isorhamnetin) were used for the optimisation and validation of the method. 
      RESULTS: The extraction and hydrolysis were accomplished in one step. The 
      procedure completes in 2 h with silica gel as dispersant, a 1:2 ratio of sample 
      to dispersant, and 60% aqueous ethanol with 0.3 M hydrochloric acid as the 
      extraction solution. The relative standard deviations (RSDs) of repeatability 
      were less than 5%, and the recoveries at two fortified levels were between 88.3 
      and 104.8%. CONCLUSION: The proposed methodology is simple and highly efficient, 
      with good repeatability and recovery. Compared with currently available methods, 
      the present work has advantages of using less time and labour, higher extraction 
      efficiency, and less consumption of the acid catalyst. This method may have 
      applications for the one-step extraction and hydrolysis of bioactive compounds 
      from plant materials. Copyright (c) 2017 John Wiley & Sons, Ltd.
CI  - Copyright (c) 2017 John Wiley & Sons, Ltd.
FAU - Tu, Xijuan
AU  - Tu X
AD  - College of Bee Science, Fujian Agriculture and Forestry University, Fuzhou, P. R. 
      China.
AD  - MOE Engineering Research Center of Bee Products Processing and Application, 
      Fujian Agriculture and Forestry University, Fuzhou, P. R. China.
FAU - Ma, Shuangqin
AU  - Ma S
AD  - College of Bee Science, Fujian Agriculture and Forestry University, Fuzhou, P. R. 
      China.
AD  - MOE Engineering Research Center of Bee Products Processing and Application, 
      Fujian Agriculture and Forestry University, Fuzhou, P. R. China.
FAU - Gao, Zhaosheng
AU  - Gao Z
AD  - College of Bee Science, Fujian Agriculture and Forestry University, Fuzhou, P. R. 
      China.
AD  - MOE Engineering Research Center of Bee Products Processing and Application, 
      Fujian Agriculture and Forestry University, Fuzhou, P. R. China.
FAU - Wang, Jing
AU  - Wang J
AD  - College of Bee Science, Fujian Agriculture and Forestry University, Fuzhou, P. R. 
      China.
AD  - MOE Engineering Research Center of Bee Products Processing and Application, 
      Fujian Agriculture and Forestry University, Fuzhou, P. R. China.
FAU - Huang, Shaokang
AU  - Huang S
AD  - College of Bee Science, Fujian Agriculture and Forestry University, Fuzhou, P. R. 
      China.
AD  - MOE Engineering Research Center of Bee Products Processing and Application, 
      Fujian Agriculture and Forestry University, Fuzhou, P. R. China.
FAU - Chen, Wenbin
AU  - Chen W
AUID- ORCID: 0000-0003-3535-9649
AD  - College of Bee Science, Fujian Agriculture and Forestry University, Fuzhou, P. R. 
      China.
AD  - MOE Engineering Research Center of Bee Products Processing and Application, 
      Fujian Agriculture and Forestry University, Fuzhou, P. R. China.
LA  - eng
PT  - Journal Article
DEP - 20170609
PL  - England
TA  - Phytochem Anal
JT  - Phytochemical analysis : PCA
JID - 9200492
RN  - 0 (Flavonoids)
RN  - 0 (Glycosides)
SB  - IM
MH  - Animals
MH  - Bees
MH  - Brassica rapa
MH  - Chemical Fractionation/*methods
MH  - Chromatography, High Pressure Liquid
MH  - Flavonoids/*chemistry
MH  - Glycosides/*chemistry
MH  - Liquid-Liquid Extraction/*methods
MH  - Pollen/*chemistry
MH  - Solid Phase Extraction/*methods
OTO - NOTNLM
OT  - HPLC-DAD
OT  - acid hydrolysis
OT  - bee pollen
OT  - flavonoid aglycones
OT  - flavonoid glycosides
OT  - matrix solid phase dispersion
OT  - sample preparation
EDAT- 2017/06/10 06:00
MHDA- 2018/01/06 06:00
CRDT- 2017/06/10 06:00
PHST- 2016/11/23 00:00 [received]
PHST- 2017/05/04 00:00 [revised]
PHST- 2017/05/08 00:00 [accepted]
PHST- 2017/06/10 06:00 [pubmed]
PHST- 2018/01/06 06:00 [medline]
PHST- 2017/06/10 06:00 [entrez]
AID - 10.1002/pca.2699 [doi]
PST - ppublish
SO  - Phytochem Anal. 2017 Nov;28(6):505-511. doi: 10.1002/pca.2699. Epub 2017 Jun 9.