PMID- 28598327
OWN - NLM
STAT- MEDLINE
DCOM- 20180219
LR  - 20181113
IS  - 2050-084X (Electronic)
IS  - 2050-084X (Linking)
VI  - 6
DP  - 2017 Jun 9
TI  - Co-agonists differentially tune GluN2B-NMDA receptor trafficking at hippocampal 
      synapses.
LID - e25492 [pii]
LID - 10.7554/eLife.25492 [doi]
AB  - The subunit composition of synaptic NMDA receptors (NMDAR), such as the relative 
      content of GluN2A- and GluN2B-containing receptors, greatly influences the 
      glutamate synaptic transmission. Receptor co-agonists, glycine and D-serine, have 
      intriguingly emerged as potential regulators of the receptor trafficking in 
      addition to their requirement for its activation. Using a combination of 
      single-molecule imaging, biochemistry and electrophysiology, we show that glycine 
      and D-serine relative availability at rat hippocampal glutamatergic synapses 
      regulate the trafficking and synaptic content of NMDAR subtypes. Acute 
      manipulations of co-agonist levels, both ex vivo and in vitro, unveil that 
      D-serine alter the membrane dynamics and content of GluN2B-NMDAR, but not 
      GluN2A-NMDAR, at synapses through a process requiring PDZ binding scaffold 
      partners. In addition, using FRET-based FLIM approach, we demonstrate that 
      D-serine rapidly induces a conformational change of the GluN1 subunit 
      intracellular C-terminus domain. Together our data fuels the view that the 
      extracellular microenvironment regulates synaptic NMDAR signaling.
FAU - Ferreira, Joana S
AU  - Ferreira JS
AUID- ORCID: 0000-0002-1049-8063
AD  - Interdisciplinary Institute for NeuroSciences, CNRS UMR 5297, Bordeaux, France.
AD  - Universite de Bordeaux, Bordeaux, France.
FAU - Papouin, Thomas
AU  - Papouin T
AD  - Universite de Bordeaux, Bordeaux, France.
AD  - NSERM U862, Neurocentre Magendie, Bordeaux, France.
FAU - Ladepeche, Laurent
AU  - Ladepeche L
AD  - Interdisciplinary Institute for NeuroSciences, CNRS UMR 5297, Bordeaux, France.
AD  - Universite de Bordeaux, Bordeaux, France.
FAU - Yao, Andrea
AU  - Yao A
AD  - Institut de Biologie de l'ENS (IBENS), CNRS UMR 8197, INSERM U1024, Paris, 
      France.
FAU - Langlais, Valentin C
AU  - Langlais VC
AD  - Universite de Bordeaux, Bordeaux, France.
AD  - NSERM U862, Neurocentre Magendie, Bordeaux, France.
FAU - Bouchet, Delphine
AU  - Bouchet D
AD  - Interdisciplinary Institute for NeuroSciences, CNRS UMR 5297, Bordeaux, France.
AD  - Universite de Bordeaux, Bordeaux, France.
FAU - Dulong, Jerome
AU  - Dulong J
AD  - Universite de Bordeaux, Bordeaux, France.
AD  - NSERM U862, Neurocentre Magendie, Bordeaux, France.
FAU - Mothet, Jean-Pierre
AU  - Mothet JP
AD  - Universite Aix-Marseille, CNRS CRN2M UMR 7286, Marseille, France.
FAU - Sacchi, Silvia
AU  - Sacchi S
AD  - Dipartimento di Biotecnologie e Scienze della Vita, Universita degli Studi 
      dell'Insubria, Varese, Italy.
AD  - The Protein Factory, Centro Interuniversitario di Biotecnologie Proteiche, 
      Politecnico di Milano, Universita degli Studi dell'Insubria, Varese, Italy.
FAU - Pollegioni, Loredano
AU  - Pollegioni L
AD  - Dipartimento di Biotecnologie e Scienze della Vita, Universita degli Studi 
      dell'Insubria, Varese, Italy.
AD  - The Protein Factory, Centro Interuniversitario di Biotecnologie Proteiche, 
      Politecnico di Milano, Universita degli Studi dell'Insubria, Varese, Italy.
FAU - Paoletti, Pierre
AU  - Paoletti P
AUID- ORCID: 0000-0002-3681-4845
AD  - Institut de Biologie de l'ENS (IBENS), CNRS UMR 8197, INSERM U1024, Paris, 
      France.
FAU - Oliet, Stephane Henri Richard
AU  - Oliet SHR
AUID- ORCID: 0000-0003-0595-9029
AD  - Universite de Bordeaux, Bordeaux, France.
AD  - NSERM U862, Neurocentre Magendie, Bordeaux, France.
FAU - Groc, Laurent
AU  - Groc L
AUID- ORCID: 0000-0003-1814-8145
AD  - Interdisciplinary Institute for NeuroSciences, CNRS UMR 5297, Bordeaux, France.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20170609
PL  - England
TA  - Elife
JT  - eLife
JID - 101579614
RN  - 0 (NR2B NMDA receptor)
RN  - 0 (Receptors, N-Methyl-D-Aspartate)
RN  - 452VLY9402 (Serine)
RN  - TE7660XO1C (Glycine)
SB  - IM
MH  - Animals
MH  - Glycine/*metabolism
MH  - Hippocampus/*physiology
MH  - Rats
MH  - Receptors, N-Methyl-D-Aspartate/*agonists
MH  - Serine/*metabolism
MH  - Synapses/*physiology
PMC - PMC5466419
OTO - NOTNLM
OT  - NMDA
OT  - neuroscience
OT  - none
OT  - subunit trafficking
OT  - synapse
COIS- The authors declare that no competing interests exist.
EDAT- 2017/06/10 06:00
MHDA- 2018/02/20 06:00
PMCR- 2017/06/10
CRDT- 2017/06/10 06:00
PHST- 2017/01/26 00:00 [received]
PHST- 2017/05/29 00:00 [accepted]
PHST- 2017/06/10 06:00 [entrez]
PHST- 2017/06/10 06:00 [pubmed]
PHST- 2018/02/20 06:00 [medline]
PHST- 2017/06/10 00:00 [pmc-release]
AID - e25492 [pii]
AID - 25492 [pii]
AID - 10.7554/eLife.25492 [doi]
PST - epublish
SO  - Elife. 2017 Jun 9;6:e25492. doi: 10.7554/eLife.25492.