PMID- 28598964
OWN - NLM
STAT- MEDLINE
DCOM- 20180319
LR  - 20240326
IS  - 1643-3750 (Electronic)
IS  - 1234-1010 (Print)
IS  - 1234-1010 (Linking)
VI  - 23
DP  - 2017 Jun 9
TI  - Changes in Expression of Dopamine, Its Receptor, and Transporter in Nucleus 
      Accumbens of Heroin-Addicted Rats with Brain-Derived Neurotrophic Factor (BDNF) 
      Overexpression.
PG  - 2805-2815
AB  - BACKGROUND The aim of this study was to explore how changes in the expression of 
      BDNF in MLDS change the effect of BDNF on dopamine (DA) neurons, which may have 
      therapeutic implications for heroin addiction. MATERIAL AND METHODS We 
      established a rat model of heroin addiction and observed changes in the 
      expression of BDNF, DA, dopamine receptor (DRD), dopamine transporter (DAT), and 
      other relevant pathways in NAc. We also assessed the effect of BDNF 
      overexpression in the NAc, behavioral changes of heroin-conditioned place 
      preference (CPP), and naloxone withdrawal in rats with high levels of BDNF. We 
      established 5 adult male rat groups: heroin addiction, lentivirus transfection, 
      blank virus, sham operation, and control. The PCR gene chip was used to study 
      gene expression changes. BDNF lentivirus transfection was used for BDNF 
      overexpression. A heroin CPP model and a naloxone withdrawal model of rats were 
      established. RESULTS Expression changes were found in 20 of the 84 DA-associated 
      genes in the NAc of heroin-addicted rats. Weight loss and withdrawal symptoms in 
      the lentivirus group for naloxone withdrawal was less than in the blank virus and 
      the sham operation group. These 2 latter groups also showed significant 
      behavioral changes, but such changes were not observed in the BDNF lentivirus 
      group before or after training. DRD3 and DAT increased in the NAc of the 
      lentivirus group. CONCLUSIONS BDNF and DA in the NAc are involved in heroin 
      addiction. BDNF overexpression in NAc reduces withdrawal symptoms and craving 
      behavior for medicine induced by environmental cues for heroin-addicted rats. 
      BDNF participates in the regulation of the dopamine system by acting on DRD3 and 
      DAT.
FAU - Li, Yixin
AU  - Li Y
AD  - Department of Histology and Embryology, Guizhou Medical University, Guiyang, 
      Guizhou, China (mainland).
FAU - Xia, Baijuan
AU  - Xia B
AD  - Department of Histology and Embryology, Guizhou Medical University, Guiyang, 
      Guizhou, China (mainland).
FAU - Li, Rongrong
AU  - Li R
AD  - Department of Histology and Embryology, Guizhou Medical University, Guiyang, 
      Guizhou, China (mainland).
FAU - Yin, Dan
AU  - Yin D
AD  - Department of Histology and Embryology, Guizhou Medical University, Guiyang, 
      Guizhou, China (mainland).
FAU - Liang, Wenmei
AU  - Liang W
AD  - Department of Histology and Embryology, Guizhou Medical University, Guiyang, 
      Guizhou, China (mainland).
LA  - eng
PT  - Journal Article
DEP - 20170609
PL  - United States
TA  - Med Sci Monit
JT  - Medical science monitor : international medical journal of experimental and 
      clinical research
JID - 9609063
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Dopamine Plasma Membrane Transport Proteins)
RN  - 0 (Receptors, Dopamine)
RN  - 70D95007SX (Heroin)
RN  - EC 1.14.16.2 (Tyrosine 3-Monooxygenase)
RN  - VTD58H1Z2X (Dopamine)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/*metabolism
MH  - Dopamine/*metabolism
MH  - Dopamine Plasma Membrane Transport Proteins/genetics/*metabolism
MH  - *Gene Expression Regulation
MH  - Heroin/*adverse effects
MH  - Male
MH  - Nucleus Accumbens/*metabolism
MH  - Plasmids/metabolism
MH  - Rats, Sprague-Dawley
MH  - Receptors, Dopamine/genetics/*metabolism
MH  - Sequence Analysis, DNA
MH  - Substance Withdrawal Syndrome/genetics
MH  - Substance-Related Disorders/*genetics
MH  - Time Factors
MH  - Tyrosine 3-Monooxygenase/genetics/metabolism
PMC - PMC5473376
EDAT- 2017/06/10 06:00
MHDA- 2018/03/20 06:00
PMCR- 2017/06/09
CRDT- 2017/06/10 06:00
PHST- 2017/06/10 06:00 [entrez]
PHST- 2017/06/10 06:00 [pubmed]
PHST- 2018/03/20 06:00 [medline]
PHST- 2017/06/09 00:00 [pmc-release]
AID - 904670 [pii]
AID - 10.12659/msm.904670 [doi]
PST - epublish
SO  - Med Sci Monit. 2017 Jun 9;23:2805-2815. doi: 10.12659/msm.904670.