PMID- 28599462
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201001
IS  - 1792-1074 (Print)
IS  - 1792-1082 (Electronic)
IS  - 1792-1074 (Linking)
VI  - 13
IP  - 6
DP  - 2017 Jun
TI  - Detection of Tax-specific CTLs in lymph nodes of adult T-cell leukemia/lymphoma 
      patients and its association with Foxp3 positivity of regulatory T-cell function.
PG  - 4611-4618
LID - 10.3892/ol.2017.6067 [doi]
AB  - Human T-cell lymphotropic virus type (HTLV)-1 Tax is a viral protein that has 
      been reported to be important in the proliferation of adult T-cell 
      leukemia/lymphoma (ATLL) cells and to be a target of HTLV-1-specific cytotoxic T 
      lymphocytes (CTLs). However, it is not clear how Tax-specific CTLs behave in 
      lymph nodes of ATLL patients. The present study analyzed the immunostaining of 
      Tax-specific CTLs. Furthermore, ATLL tumor cells are known to be positive for 
      forkhead box P3 (Foxp3)and to have a regulatory T (Treg)-cell-like function. The 
      association between T-reg function and number and activity of Tax-specific CTLs 
      was also investigated. A total of 15 ATLL lymphoma cases with human leukocyte 
      antigen (HLA)-A24, for which Tax has a high affinity, were selected from the 
      files of the Department of Pathology, School of Medicine, Kurume University 
      (Kurume, Japan) using a polymerase chain reaction (PCR) method. Immunostaining 
      was performed for cluster of differentiation (CD) 20, CD3, CD4, CD8, T-cell 
      intracellular antigen-1 and Foxp3 in paraffin sections, and for Tax, interferon gamma 
      and HLA-A24 in frozen sections. In addition, the staining of Tax-specific CTLs 
      (HLA-A24-restricted) was analyzed by MHC Dextramer((R)) assay in frozen sections. 
      In addition, the messenger RNA expression of Tax and HTLV-1 basic leucine zipper 
      factor were also evaluated by reverse transcription-PCR. Immunohistochemical 
      staining of Tax protein in lymphoma tissue revealed the presence of positive 
      lymphoma cells ranging from 5 to 80%, and immunohistochemical staining of HLA-A24 
      revealed the presence of positive lymphoma cells ranging from 1 to 95%. The 
      expression of Tax and HLA-A24 was downregulated by viral function. Foxp3, a 
      marker for Treg cells, was expressed in 0-90% of cells. Several cases exhibited 
      Tax-specific CTL (HLA-A24-restricted)-positive cells, and there was an inverse 
      correlation between Tax-specific CTLs and Foxp3. However, neither Tax nor HLA-A24 
      expression was associated with CTL or Foxp3. Our study indicated the possibility 
      that ATLL cells, which expressed Tax, target of CTL, evade the CTL-mediated 
      immune control by expression of Foxp3 as a Treg function.
FAU - Ichikawa, Ayako
AU  - Ichikawa A
AD  - Department of Pathology, School of Medicine, Kurume University, Kurume, Fukuoka 
      830 0011, Japan.
FAU - Miyoshi, Hiroaki
AU  - Miyoshi H
AD  - Department of Pathology, School of Medicine, Kurume University, Kurume, Fukuoka 
      830 0011, Japan.
FAU - Arakawa, Fumiko
AU  - Arakawa F
AD  - Department of Pathology, School of Medicine, Kurume University, Kurume, Fukuoka 
      830 0011, Japan.
FAU - Kiyasu, Junichi
AU  - Kiyasu J
AD  - Department of Pathology, School of Medicine, Kurume University, Kurume, Fukuoka 
      830 0011, Japan.
FAU - Sato, Kensaku
AU  - Sato K
AD  - Biostatistics Center, School of Medicine, Kurume University, Kurume, Fukuoka 830 
      0011, Japan.
FAU - Niino, Daisuke
AU  - Niino D
AD  - Department of Pathology, School of Medicine, Kurume University, Kurume, Fukuoka 
      830 0011, Japan.
FAU - Kimura, Yoshizo
AU  - Kimura Y
AD  - Department of Pathology, School of Medicine, Kurume University, Kurume, Fukuoka 
      830 0011, Japan.
FAU - Yoshida, Maki
AU  - Yoshida M
AD  - Department of Pathology, School of Medicine, Kurume University, Kurume, Fukuoka 
      830 0011, Japan.
FAU - Kawano, Riko
AU  - Kawano R
AD  - Department of Pathology, School of Medicine, Kurume University, Kurume, Fukuoka 
      830 0011, Japan.
FAU - Muta, Hiroko
AU  - Muta H
AD  - Department of Pathology, School of Medicine, Kurume University, Kurume, Fukuoka 
      830 0011, Japan.
FAU - Sugita, Yasuo
AU  - Sugita Y
AD  - Department of Pathology, School of Medicine, Kurume University, Kurume, Fukuoka 
      830 0011, Japan.
FAU - Ohshima, Koichi
AU  - Ohshima K
AD  - Department of Pathology, School of Medicine, Kurume University, Kurume, Fukuoka 
      830 0011, Japan.
LA  - eng
PT  - Journal Article
DEP - 20170421
PL  - Greece
TA  - Oncol Lett
JT  - Oncology letters
JID - 101531236
PMC - PMC5453040
OTO - NOTNLM
OT  - Foxp3
OT  - Tax-specific cytotoxic T-cell
OT  - adult T-cell leukemia/lymphoma
OT  - human T-cell lymphotropic virus
OT  - regulatory T-cell
EDAT- 2017/06/11 06:00
MHDA- 2017/06/11 06:01
PMCR- 2017/04/21
CRDT- 2017/06/11 06:00
PHST- 2015/05/12 00:00 [received]
PHST- 2016/09/27 00:00 [accepted]
PHST- 2017/06/11 06:00 [entrez]
PHST- 2017/06/11 06:00 [pubmed]
PHST- 2017/06/11 06:01 [medline]
PHST- 2017/04/21 00:00 [pmc-release]
AID - OL-0-0-6067 [pii]
AID - 10.3892/ol.2017.6067 [doi]
PST - ppublish
SO  - Oncol Lett. 2017 Jun;13(6):4611-4618. doi: 10.3892/ol.2017.6067. Epub 2017 Apr 
      21.