PMID- 28600195
OWN - NLM
STAT- MEDLINE
DCOM- 20171218
LR  - 20171218
IS  - 1095-9947 (Electronic)
IS  - 1050-4648 (Linking)
VI  - 67
DP  - 2017 Aug
TI  - Identification and expression analysis of two pro-inflammatory cytokines, TNF-alpha 
      and IL-8, in cobia (Rachycentron canadum L.) in response to Streptococcus 
      dysgalactiae infection.
PG  - 159-171
LID - S1050-4648(17)30335-2 [pii]
LID - 10.1016/j.fsi.2017.06.014 [doi]
AB  - Tumor necrosis factor-alpha (TNF-alpha) and interleukin-8 (IL-8/CXCL8) play pivotal 
      roles in mediating inflammatory responses to invading pathogens. In this study, 
      we identified and analyzed expressions of cobia TNF-alpha and IL-8 during 
      Streptococcus dysgalactiae infection. The cloned cDNA transcript of cobia TNF-alpha 
      comprised of 1281 base pairs (bp), with a 774 bp open reading frame (ORF) 
      encoding 257 amino acids. The deduced amino acid sequence of cobia TNF-alpha showed a 
      close relationship (84% similarity) with TNF-alpha of yellowtail amberjack. The 
      cloned IL-8 cDNA sequence was 828 bp long, including a 300-bp ORF encoding 99 
      amino acids. The deduced amino acid sequence of cobia IL-8 shared 90% identity 
      with IL-8 of striped trumpeter. Cobia challenged with a virulent S. dysgalactiae 
      strain displayed an early significant up-regulation of TNF-alpha and IL-8 in head 
      kidney, liver, and spleen. Notably, IL-8 expression level increased dramatically 
      in the liver at the severe stage of infection (72 h). In conclusion, a better 
      understanding of TNF-alpha and IL-8 allows more detailed investigation of immune 
      responses in cobia and furthers study on controlling the infectious disease 
      caused by S. dysgalactiae.
CI  - Copyright (c) 2017 Elsevier Ltd. All rights reserved.
FAU - Nguyen, Thuy Thi Thu
AU  - Nguyen TTT
AD  - Department of Veterinary Medicine, College of Veterinary Medicine, National 
      Pingtung University of Science and Technology, No. 1, Shuefu Road, Neipu, 
      Pingtung 91201, Taiwan.
FAU - Nguyen, Hai Trong
AU  - Nguyen HT
AD  - Department of Veterinary Medicine, College of Veterinary Medicine, National 
      Pingtung University of Science and Technology, No. 1, Shuefu Road, Neipu, 
      Pingtung 91201, Taiwan.
FAU - Wang, Pei-Chyi
AU  - Wang PC
AD  - Department of Veterinary Medicine, College of Veterinary Medicine, National 
      Pingtung University of Science and Technology, No. 1, Shuefu Road, Neipu, 
      Pingtung 91201, Taiwan. Electronic address: pc921003@gmail.com.
FAU - Chen, Shih-Chu
AU  - Chen SC
AD  - Department of Veterinary Medicine, College of Veterinary Medicine, National 
      Pingtung University of Science and Technology, No. 1, Shuefu Road, Neipu, 
      Pingtung 91201, Taiwan; International Degree Program of Ornamental Fish Science 
      and Technology, International College, National Pingtung University of Science 
      and Technology, No. 1, Shuefu Road, Neipu, Pingtung 91201, Taiwan. Electronic 
      address: scchen@mail.npust.edu.tw.
LA  - eng
PT  - Journal Article
DEP - 20170612
PL  - England
TA  - Fish Shellfish Immunol
JT  - Fish & shellfish immunology
JID - 9505220
RN  - 0 (Fish Proteins)
RN  - 0 (Interleukin-8)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Fish Diseases/*immunology
MH  - Fish Proteins/chemistry/*genetics/immunology
MH  - Gene Expression Regulation/*immunology
MH  - Immunity, Innate/*genetics
MH  - Interleukin-8/chemistry/*genetics/immunology
MH  - *Perciformes
MH  - Phylogeny
MH  - Sequence Alignment/veterinary
MH  - Streptococcal Infections/immunology
MH  - Streptococcus/physiology
MH  - Tumor Necrosis Factor-alpha/chemistry/*genetics/immunology
OTO - NOTNLM
OT  - Cobia
OT  - IL-8
OT  - Rachycentron canadum
OT  - Streptococcus dysgalactiae
OT  - TNF-alpha
EDAT- 2017/06/11 06:00
MHDA- 2017/12/19 06:00
CRDT- 2017/06/11 06:00
PHST- 2017/02/07 00:00 [received]
PHST- 2017/06/04 00:00 [revised]
PHST- 2017/06/05 00:00 [accepted]
PHST- 2017/06/11 06:00 [pubmed]
PHST- 2017/12/19 06:00 [medline]
PHST- 2017/06/11 06:00 [entrez]
AID - S1050-4648(17)30335-2 [pii]
AID - 10.1016/j.fsi.2017.06.014 [doi]
PST - ppublish
SO  - Fish Shellfish Immunol. 2017 Aug;67:159-171. doi: 10.1016/j.fsi.2017.06.014. Epub 
      2017 Jun 12.