PMID- 28602824
OWN - NLM
STAT- MEDLINE
DCOM- 20180618
LR  - 20180618
IS  - 1618-0402 (Electronic)
IS  - 0940-9602 (Linking)
VI  - 213
DP  - 2017 Sep
TI  - Distribution of BDNF and TrkB isoforms in growing antler tissues of red deer.
PG  - 33-46
LID - S0940-9602(17)30081-X [pii]
LID - 10.1016/j.aanat.2017.05.007 [doi]
AB  - Antlers are the cranial appendages of deer that regenerate each year. This 
      renewal provides a model to explore molecules involved in mammalian organ 
      regeneration. The cellular distributions of the brain-derived neurotrophic factor 
      (BDNF) and the isoforms of its cognate receptor Trk tyrosine kinase receptor 
      (TrkB) were localized by immunohistochemistry in sections of growing red deer 
      antler. BDNF and TrkB full length were widely expressed in the integument, 
      perichondrium, periosteum and bone. The truncated isoform receptor was 
      particularly evidenced in integument and vascular inner dermis, but very light 
      reaction was observed in cartilage and bone, both at the site of endochondral and 
      intramembranous ossification. These observations were also assessed at 
      transcriptional level by RT-PCR analyses. The highest expression of all genes 
      significantly occurred in chondroprogenitor cells; however the full-length TrkB 
      receptor was down regulated in osteocartilaginous compartments, in which the 
      truncated isoform was up regulated. The truncated isoform is a dominant-negative 
      receptor that inhibits the full length receptor signalling, even if the truncated 
      isoform not only has this function. This study establishes the presence of BDNF 
      and its receptor in the different cellular compartments of growing antler. Their 
      transcripts assessed by RT-PCR indicate a local synthesis of these molecules that 
      may contribute to the modulation of antler growth, acting as autocrine and/or 
      paracrine factors independently of nerve supply. Among the plethora of other 
      molecular signals and growth factors affecting the antler growth, the local 
      production of BDNF and its cognate receptor could be of interest in understanding 
      their role in antler renewal and to delineate the different involvement of the 
      receptor isoforms.
CI  - Copyright (c) 2017 Elsevier GmbH. All rights reserved.
FAU - Colitti, M
AU  - Colitti M
AD  - Department of Agricultural, Food, Environmental and Animal Sciences, University 
      of Udine, via delle Scienze, 206, 33100 Udine, Italy. Electronic address: 
      monica.colitti@uniud.it.
LA  - eng
PT  - Journal Article
DEP - 20170607
PL  - Germany
TA  - Ann Anat
JT  - Annals of anatomy = Anatomischer Anzeiger : official organ of the Anatomische 
      Gesellschaft
JID - 100963897
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - EC 2.7.10.1 (Receptor, trkB)
SB  - IM
MH  - Animals
MH  - Antlers/cytology/*metabolism
MH  - Bone and Bones/metabolism
MH  - Brain-Derived Neurotrophic Factor/*metabolism
MH  - Cartilage/metabolism
MH  - Chondrocytes/physiology
MH  - Deer/*metabolism
MH  - Dermis/metabolism
MH  - Humans
MH  - Immunohistochemistry
MH  - Isomerism
MH  - Male
MH  - Osteogenesis
MH  - Receptor, trkB/*metabolism
MH  - Signal Transduction
MH  - Stem Cells/metabolism
OTO - NOTNLM
OT  - Antler
OT  - BDNF
OT  - Expression
OT  - Isoform
OT  - TrkB
EDAT- 2017/06/13 06:00
MHDA- 2018/06/19 06:00
CRDT- 2017/06/13 06:00
PHST- 2017/02/24 00:00 [received]
PHST- 2017/05/16 00:00 [revised]
PHST- 2017/05/16 00:00 [accepted]
PHST- 2017/06/13 06:00 [pubmed]
PHST- 2018/06/19 06:00 [medline]
PHST- 2017/06/13 06:00 [entrez]
AID - S0940-9602(17)30081-X [pii]
AID - 10.1016/j.aanat.2017.05.007 [doi]
PST - ppublish
SO  - Ann Anat. 2017 Sep;213:33-46. doi: 10.1016/j.aanat.2017.05.007. Epub 2017 Jun 7.