PMID- 28604758
OWN - NLM
STAT- MEDLINE
DCOM- 20180723
LR  - 20181113
IS  - 1530-0447 (Electronic)
IS  - 0031-3998 (Linking)
VI  - 82
IP  - 4
DP  - 2017 Oct
TI  - Intrauterine growth retardation-associated syncytin b hypermethylation in 
      maternal rat blood revealed by DNA methylation array analysis.
PG  - 704-711
LID - 10.1038/pr.2017.137 [doi]
AB  - BackgroundEmerging evidence suggests that DNA methylation in maternal blood is a 
      promising target for intrauterine growth retardation (IUGR) screening, a common 
      developmental toxicity. Here, we aimed to screen out IUGR-related DNA methylation 
      status in maternal blood via high-throughput profiling.MethodsPregnant Wistar 
      rats were subcutaneously administered nicotine (1 mg/kg) twice per day from 
      gestational day (GD) 11 to GD20 to establish the IUGR model. MeDIP array assays 
      and the following GO analysis were used to evaluate DNA methylation status in 
      maternal blood. One placental development-associated gene was selected for 
      further confirmation.ResultsGenes regulating the development of multiple organs 
      and major body systems had changed DNA methylation frequencies in the maternal 
      blood of IUGR rats. Placental development, which can affect the development of 
      multiple fetal organs and induce IUGR, is a hypermethylated cluster consisting of 
      four significantly changed genes, including syncytin b (Synb), Lrrc15, Met, and 
      Tex19.1. With the most significant change, Synb hypermethylation in maternal 
      blood was confirmed by bisulfite-sequencing PCR (BSP). Moreover, decreased Synb 
      expression and histological changes were observed in IUGR placentae.ConclusionThe 
      IUGR-associated DNA methylation profile in maternal blood, such as 
      placenta-related Synb hypermethylation, provides evidence for further studies on 
      possible IUGR biomarkers.
FAU - Wu, Dong-Mei
AU  - Wu DM
AD  - Department of Pharmacology, Wuhan University School of Basic Medical Sciences, 
      Wuhan, Huibei, China.
FAU - Yan, You-E
AU  - Yan YE
AD  - Department of Pharmacology, Wuhan University School of Basic Medical Sciences, 
      Wuhan, Huibei, China.
FAU - Ma, Liang-Peng
AU  - Ma LP
AD  - Department of Pharmacy, Wuhan First Hospital, Wuhan, Hubei, China.
FAU - Liu, Han-Xiao
AU  - Liu HX
AD  - Department of Pharmacology, Wuhan University School of Basic Medical Sciences, 
      Wuhan, Huibei, China.
FAU - Qu, Wen
AU  - Qu W
AD  - Department of Pharmacology, Wuhan University School of Basic Medical Sciences, 
      Wuhan, Huibei, China.
FAU - Ping, Jie
AU  - Ping J
AD  - Department of Pharmacology, Wuhan University School of Basic Medical Sciences, 
      Wuhan, Huibei, China.
LA  - eng
PT  - Journal Article
DEP - 20170705
PL  - United States
TA  - Pediatr Res
JT  - Pediatric research
JID - 0100714
RN  - 0 (Pregnancy Proteins)
RN  - 0 (Synb protein, rat)
RN  - 6M3C89ZY6R (Nicotine)
SB  - IM
MH  - Animals
MH  - *DNA Methylation
MH  - Disease Models, Animal
MH  - *Epigenesis, Genetic
MH  - Female
MH  - Fetal Growth Retardation/blood/chemically induced/*genetics
MH  - Gene Expression Regulation, Developmental
MH  - High-Throughput Nucleotide Sequencing
MH  - Nicotine
MH  - *Oligonucleotide Array Sequence Analysis
MH  - Placenta/metabolism
MH  - Pregnancy
MH  - Pregnancy Proteins/blood/*genetics
MH  - Promoter Regions, Genetic
MH  - Rats, Wistar
EDAT- 2017/06/13 06:00
MHDA- 2018/07/24 06:00
CRDT- 2017/06/13 06:00
PHST- 2016/10/01 00:00 [received]
PHST- 2017/05/28 00:00 [accepted]
PHST- 2017/06/13 06:00 [pubmed]
PHST- 2018/07/24 06:00 [medline]
PHST- 2017/06/13 06:00 [entrez]
AID - pr2017137 [pii]
AID - 10.1038/pr.2017.137 [doi]
PST - ppublish
SO  - Pediatr Res. 2017 Oct;82(4):704-711. doi: 10.1038/pr.2017.137. Epub 2017 Jul 5.