PMID- 28604954
OWN - NLM
STAT- MEDLINE
DCOM- 20170914
LR  - 20191210
IS  - 1003-9406 (Print)
IS  - 1003-9406 (Linking)
VI  - 34
IP  - 3
DP  - 2017 Jun 10
TI  - [Detection of genomic abnormalities among 105 patients with chronic lymphocytic 
      leukemia using fluorescence in situ hybridization].
PG  - 357-360
LID - 10.3760/cma.j.issn.1003-9406.2017.03.009 [doi]
AB  - OBJECTIVE: To assess the value of fluorescence in situ hybridization (FISH) for 
      the detection of genomic abnormalities among patients with chronic lymphocytic 
      leukemia (CLL). METHODS: Interphase FISH was performed on bone marrow samples 
      derived from 105 patients with CLL at the time of diagnosis using probes for 
      D13S319/13q14, ATM/11q22, P53/17p13 and CEP12. The abnormalities and prognostic 
      factors were analyzed. Overall survival of the patients was calculated. RESULTS: 
      The FISH assay has detected genomic abnormalities in 81 (77.1%) of the patients, 
      among which D13S319/13q14 deletion was the most common (49/105, 46.67%). 
      24(22.86%) patients had trisomy 12, 21(20.00%) had ATM/11q deletion, and 
      12(11.43%) had P53/17p deletion. A significant correlation was found between 
      Binet staging and the detected abnormalities (< 0.05). With a median follow-up 
      time of 10 months, 11 patients (10.5%) had died. Compared with those with P53 
      deletion, patients with 13q deletion showed a better overall survival. However, 
      the overall survival did not significantly differ between patients with various 
      genomic abnormalities (> 0.05). CONCLUSION: FISH is capable of detecting common 
      genomic aberrations among patients with newly diagnosed CLL. Deletion of 
      D13S319/13q14 is the most common aberration in such patients. Genomic aberrations 
      are significantly correlated with Binet staging but not the overall survival of 
      CLL patients.
FAU - Wang, Huanping
AU  - Wang H
AD  - Institute of Hematology, Key Laboratory of Hematologic Malignancies of Zhejiang 
      Province, the First Affiliated Hospital, College of Medicine, Zhejiang 
      University, Hangzhou, Zhejiang 310009, China. jiej0503@163.com.
FAU - Xu, Huan
AU  - Xu H
FAU - Chen, Zhimei
AU  - Chen Z
FAU - Lou, Jiyu
AU  - Lou J
FAU - Jin, Jie
AU  - Jin J
LA  - chi
PT  - Evaluation Study
PT  - Journal Article
PL  - China
TA  - Zhonghua Yi Xue Yi Chuan Xue Za Zhi
JT  - Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese 
      journal of medical genetics
JID - 9425197
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - *Chromosome Aberrations
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Leukemia, Lymphocytic, Chronic, B-Cell/diagnosis/*genetics
MH  - Male
MH  - Middle Aged
EDAT- 2017/06/13 06:00
MHDA- 2017/09/15 06:00
CRDT- 2017/06/13 06:00
PHST- 2017/06/13 06:00 [entrez]
PHST- 2017/06/13 06:00 [pubmed]
PHST- 2017/09/15 06:00 [medline]
AID - 940634070 [pii]
AID - 10.3760/cma.j.issn.1003-9406.2017.03.009 [doi]
PST - ppublish
SO  - Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2017 Jun 10;34(3):357-360. doi: 
      10.3760/cma.j.issn.1003-9406.2017.03.009.