PMID- 28605624
OWN - NLM
STAT- MEDLINE
DCOM- 20170915
LR  - 20170915
IS  - 1873-376X (Electronic)
IS  - 1570-0232 (Linking)
VI  - 1060
DP  - 2017 Aug 15
TI  - The application of chromatographic breath analysis in the search of volatile 
      biomarkers of chronic kidney disease and coexisting type 2 diabetes mellitus.
PG  - 103-110
LID - S1570-0232(17)30328-8 [pii]
LID - 10.1016/j.jchromb.2017.05.030 [doi]
AB  - Chromatographic studies on breath composition are aimed at finding volatile 
      markers useful for medical diagnostics or in screening investigations. Studies 
      leading to the development of screening breath tests are especially important for 
      the diagnostics of chronic kidney disease (CKD) and type 2 diabetes mellitus 
      (T2DM). The aim of the presented study was to confirm diagnostic usefulness of 
      chosen volatile compounds detected in breath, which are suggested as potential 
      biomarkers of renal dysfunction and diabetes. Breath analysis were carried out in 
      three groups: 10 healthy volunteers, 10 patients with CKD and 10 patients with 
      CKD and T2DM. All exhaled air samples were analyzed using gas chromatograph 
      (Agilent 6890GC) coupled with mass spectrometer (5975MSD). Thermal desorption was 
      applied as the enrichment method. TMA was detected only in CKD patients. Higher 
      breath concentrations of methanethiol (MeSH) were observed in CKD patients with 
      coexisting diabetes than in patients with renal dysfunction only or in the 
      healthy group. There was a tendency of increasing MeSH concentration in breath 
      with increasing total glutathione in plasma (r=0.53, p=0.0026). Also, a trend of 
      increasing dimethylsulfide (DMS) levels detected in breath was noticed with an 
      increase of hydrogen sulfide concentration in plasma (r=0.74; p=0.00001) as well 
      as with aspartate aminotransferase (AST), (r=0.61; p=0.001). The presented 
      results suggest the possibility of applying TMA, MeSH, and DMS detection in 
      breath as diagnostic methods.
CI  - Copyright (c) 2017 Elsevier B.V. All rights reserved.
FAU - Grabowska-Polanowska, B
AU  - Grabowska-Polanowska B
AD  - Institute of Nuclear Physics, Polish Academy of Sciences, Krakow, Poland. 
      Electronic address: beata.grabowska@ifj.edu.pl.
FAU - Skowron, M
AU  - Skowron M
AD  - Institute of Nuclear Physics, Polish Academy of Sciences, Krakow, Poland.
FAU - Miarka, P
AU  - Miarka P
AD  - Jagiellonian University Medical College, Department of Nephrology, Krakow, 
      Poland.
FAU - Pietrzycka, A
AU  - Pietrzycka A
AD  - Jagiellonian University Medical College, Faculty of Pharmacy, Krakow, Poland.
FAU - Sliwka, I
AU  - Sliwka I
AD  - Institute of Nuclear Physics, Polish Academy of Sciences, Krakow, Poland.
LA  - eng
PT  - Journal Article
DEP - 20170603
PL  - Netherlands
TA  - J Chromatogr B Analyt Technol Biomed Life Sci
JT  - Journal of chromatography. B, Analytical technologies in the biomedical and life 
      sciences
JID - 101139554
RN  - 0 (Biomarkers)
RN  - 0 (Butadienes)
RN  - 0 (Hemiterpenes)
RN  - 0 (Methylamines)
RN  - 0 (Pentanes)
RN  - 0 (Sulfides)
RN  - 0 (Volatile Organic Compounds)
RN  - 0A62964IBU (isoprene)
RN  - 1364PS73AF (Acetone)
RN  - LHH7G8O305 (trimethylamine)
RN  - QS3J7O7L3U (dimethyl sulfide)
SB  - IM
MH  - Acetone/analysis
MH  - Adult
MH  - Biomarkers/*analysis
MH  - Butadienes/analysis
MH  - Case-Control Studies
MH  - Diabetes Mellitus, Type 2/*complications/*metabolism
MH  - Exhalation
MH  - Female
MH  - Hemiterpenes/analysis
MH  - Humans
MH  - Limit of Detection
MH  - Male
MH  - Methylamines/analysis
MH  - Middle Aged
MH  - Pentanes/analysis
MH  - Renal Insufficiency, Chronic/*complications/*metabolism
MH  - Reproducibility of Results
MH  - Sulfides/analysis
MH  - Volatile Organic Compounds/*analysis
OTO - NOTNLM
OT  - Biomarkers
OT  - Breath analysis
OT  - Diabetes
OT  - Gas chromatography
OT  - Renal disease
EDAT- 2017/06/13 06:00
MHDA- 2017/09/16 06:00
CRDT- 2017/06/13 06:00
PHST- 2017/02/27 00:00 [received]
PHST- 2017/05/22 00:00 [revised]
PHST- 2017/05/26 00:00 [accepted]
PHST- 2017/06/13 06:00 [pubmed]
PHST- 2017/09/16 06:00 [medline]
PHST- 2017/06/13 06:00 [entrez]
AID - S1570-0232(17)30328-8 [pii]
AID - 10.1016/j.jchromb.2017.05.030 [doi]
PST - ppublish
SO  - J Chromatogr B Analyt Technol Biomed Life Sci. 2017 Aug 15;1060:103-110. doi: 
      10.1016/j.jchromb.2017.05.030. Epub 2017 Jun 3.