PMID- 28606076
OWN - NLM
STAT- MEDLINE
DCOM- 20180104
LR  - 20221207
IS  - 1472-6750 (Electronic)
IS  - 1472-6750 (Linking)
VI  - 17
IP  - 1
DP  - 2017 Jun 12
TI  - Flocculating performance of a bioflocculant produced by Arthrobacter humicola in 
      sewage waste water treatment.
PG  - 51
LID - 10.1186/s12896-017-0375-0 [doi]
LID - 51
AB  - BACKGROUND: The discharge of poorly treated effluents into the environment has 
      far reaching, consequential impacts on human and aquatic life forms. Thus, we 
      evaluated the flocculating efficiency of our test bioflocculant and we report for 
      the first time the ability of the biopolymeric flocculant produced by 
      Arthrobacter humicola in the treatment of sewage wastewater. This strain was 
      isolated from sediment soil sample at Sterkfontein dam in the Eastern Free State 
      province of South Africa. RESULTS: Basic Local Alignment Search Tool (BLAST) 
      analysis of the nucleotide sequence of the 16S rDNA revealed the bacteria to have 
      99% similarity to Arthrobacter humicola strain R1 and the sequence was deposited 
      in the Gene bank as Arthrobacter humicola with accession number KC816574.1. 
      Flocculating activity was enhanced with the aid of divalent cations, pH 12, at a 
      dosage concentration of 0.8 mg/mL. The purified bioflocculant was heat stable and 
      could retain more than 78% of its flocculating activity after heating at 100  degrees C 
      for 25 min. Fourier Transform Infrared Spectroscopy analysis demonstrated the 
      presence of hydroxyl and carboxyl moieties as the functional groups. The 
      thermogravimetric analysis was used to monitor the pyrolysis profile of the 
      purified bioflocculant and elemental composition revealed C: O: Na: P: K with 
      13.90: 41.96: 26.79: 16.61: 0.74 weight percentage respectively. The purified 
      bioflocculant was able to remove chemical oxygen demand, biological oxygen 
      demand, suspended solids, nitrate and turbidity from sewage waste water at 
      efficiencies of 65.7%, 63.5%, 55.7%, 71.4% and 81.3% respectively. CONCLUSIONS: 
      The results of this study indicate the possibility of using the bioflocculant 
      produced by Arthrobacter humicola as a potential alternative to synthesized 
      chemical flocculants in sewage waste water treatment and other industrial waste 
      water.
FAU - Agunbiade, Mayowa Oladele
AU  - Agunbiade MO
AD  - Phytomedicine and Phytopharmacology Research Group, Department of Plant Sciences, 
      University of the Free State, Qwaqwa Campus, P. Bag X13, Phuthaditjabha, 9866, 
      South Africa. mayorlala@gmail.com.
AD  - Department of Microbial, Biochemical & Food Biotechnology, University of the Free 
      State, P.O. Box 339, Nelson Mandela Drive, Bloemfontein, 9301, South Africa. 
      mayorlala@gmail.com.
FAU - Van Heerden, Esta
AU  - Van Heerden E
AD  - Department of Microbial, Biochemical & Food Biotechnology, University of the Free 
      State, P.O. Box 339, Nelson Mandela Drive, Bloemfontein, 9301, South Africa.
FAU - Pohl, Carolina H
AU  - Pohl CH
AD  - Department of Microbial, Biochemical & Food Biotechnology, University of the Free 
      State, P.O. Box 339, Nelson Mandela Drive, Bloemfontein, 9301, South Africa.
FAU - Ashafa, Anofi Tom
AU  - Ashafa AT
AD  - Phytomedicine and Phytopharmacology Research Group, Department of Plant Sciences, 
      University of the Free State, Qwaqwa Campus, P. Bag X13, Phuthaditjabha, 9866, 
      South Africa.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20170612
PL  - England
TA  - BMC Biotechnol
JT  - BMC biotechnology
JID - 101088663
RN  - 0 (Sewage)
RN  - 0 (Waste Water)
RN  - 0 (Water Pollutants, Chemical)
SB  - IM
MH  - Arthrobacter/*chemistry/classification/growth & development/*metabolism
MH  - Biodegradation, Environmental
MH  - Flocculation
MH  - Sewage/*microbiology
MH  - *Soil Microbiology
MH  - Species Specificity
MH  - Wastewater/*microbiology
MH  - Water Pollutants, Chemical/isolation & purification/*metabolism
MH  - Water Purification/*methods
PMC - PMC5469021
OTO - NOTNLM
OT  - Arthrobacter humicola
OT  - FTIR
OT  - Flocculating activity
OT  - Purified bioflocculant
OT  - Sewage waste water
EDAT- 2017/06/14 06:00
MHDA- 2018/01/05 06:00
PMCR- 2017/06/12
CRDT- 2017/06/14 06:00
PHST- 2017/03/01 00:00 [received]
PHST- 2017/06/07 00:00 [accepted]
PHST- 2017/06/14 06:00 [entrez]
PHST- 2017/06/14 06:00 [pubmed]
PHST- 2018/01/05 06:00 [medline]
PHST- 2017/06/12 00:00 [pmc-release]
AID - 10.1186/s12896-017-0375-0 [pii]
AID - 375 [pii]
AID - 10.1186/s12896-017-0375-0 [doi]
PST - epublish
SO  - BMC Biotechnol. 2017 Jun 12;17(1):51. doi: 10.1186/s12896-017-0375-0.