PMID- 28608202
OWN - NLM
STAT- MEDLINE
DCOM- 20180319
LR  - 20181113
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Print)
IS  - 1064-3745 (Linking)
VI  - 1626
DP  - 2017
TI  - Zymography as a Research Tool in the Study of Matrix Metalloproteinase 
      Inhibitors.
PG  - 79-102
LID - 10.1007/978-1-4939-7111-4_8 [doi]
AB  - Matrix metalloproteinases (MMPs) are proteolytic enzymes that degrade various 
      components of the extracellular matrix (ECM) and play a role in tissue 
      remodeling. Changes in MMPs have been observed in cancer, connective tissue 
      disorders, and vascular disease, and both endogenous tissue inhibitors of MMPs 
      (TIMPs) and synthetic MMP inhibitors (MMPIs) have been evaluated as modulators of 
      MMP activity in various biological systems. Zymography is a simple technique that 
      is commonly used to assess MMP activity and the efficacy of MMPIs. Also, reverse 
      zymography is a modified technique to study the activity of endogenous TIMPs. 
      However, problems are often encountered during the zymography procedure, which 
      could interfere with accurate assessment of MMP activity in control specimens, 
      and thus make it difficult to determine the pathological changes in MMPs and 
      their responsiveness to MMPIs. Simplified protocols for preparation of 
      experimental solutions, tissue preparation, regular and reverse zymography 
      procedures, and zymogram analysis are presented. Additional helpful tips to 
      troubleshoot problems in the zymography technique and to enhance the quality of 
      the zymograms should make it more feasible to determine the changes in MMPs and 
      assess the efficacy of MMPIs in modulating MMP activity in various biological 
      systems and pathological conditions.
FAU - Ren, Zongli
AU  - Ren Z
AD  - Vascular Surgery Research Laboratory, Division of Vascular and Endovascular 
      Surgery, Brigham and Women's Hospital and Harvard Medical School, 75 Francis 
      Street, Boston, MA, 02115, USA.
FAU - Chen, Juanjuan
AU  - Chen J
AD  - Vascular Surgery Research Laboratory, Division of Vascular and Endovascular 
      Surgery, Brigham and Women's Hospital and Harvard Medical School, 75 Francis 
      Street, Boston, MA, 02115, USA.
FAU - Khalil, Raouf A
AU  - Khalil RA
AD  - Vascular Surgery Research Laboratory, Division of Vascular and Endovascular 
      Surgery, Brigham and Women's Hospital and Harvard Medical School, 75 Francis 
      Street, Boston, MA, 02115, USA. raouf_khalil@hms.harvard.edu.
LA  - eng
GR  - R01 HL065998/HL/NHLBI NIH HHS/United States
GR  - R21 HL098724/HL/NHLBI NIH HHS/United States
GR  - R21 HL111775/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
RN  - 0 (Tissue Inhibitor of Metalloproteinases)
RN  - EC 3.4.24.- (Matrix Metalloproteinases)
RN  - EC 3.4.24.24 (Matrix Metalloproteinase 2)
RN  - EC 3.4.24.35 (Matrix Metalloproteinase 9)
SB  - IM
MH  - Animals
MH  - Electrophoresis, Polyacrylamide Gel/*methods
MH  - Enzyme Assays/*methods
MH  - Extracellular Matrix/metabolism
MH  - Female
MH  - Humans
MH  - Matrix Metalloproteinase 2/analysis/metabolism
MH  - Matrix Metalloproteinase 9/analysis/metabolism
MH  - Matrix Metalloproteinases/analysis/*metabolism
MH  - Rats
MH  - Tissue Inhibitor of Metalloproteinases/analysis/metabolism
MH  - Uterus/enzymology/metabolism
PMC - PMC5527288
MID - NIHMS882226
OTO - NOTNLM
OT  - Blood vessels
OT  - Extracellular matrix
OT  - Matrix metalloproteinase
OT  - TIMP
OT  - Uterus
EDAT- 2017/06/14 06:00
MHDA- 2018/03/20 06:00
PMCR- 2018/01/01
CRDT- 2017/06/14 06:00
PHST- 2017/06/14 06:00 [entrez]
PHST- 2017/06/14 06:00 [pubmed]
PHST- 2018/03/20 06:00 [medline]
PHST- 2018/01/01 00:00 [pmc-release]
AID - 10.1007/978-1-4939-7111-4_8 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2017;1626:79-102. doi: 10.1007/978-1-4939-7111-4_8.