PMID- 28608905
OWN - NLM
STAT- MEDLINE
DCOM- 20181126
LR  - 20181126
IS  - 1364-5528 (Electronic)
IS  - 0003-2654 (Linking)
VI  - 142
IP  - 14
DP  - 2017 Jul 10
TI  - Detection of SiO(2) nanoparticles in lung tissue by ToF-SIMS imaging and 
      fluorescence microscopy.
PG  - 2631-2639
LID - 10.1039/c7an00399d [doi]
AB  - The direct detection of nanoparticles in tissues at high spatial resolution is a 
      current goal in nanotoxicology. Time-of-Flight Secondary Ion Mass Spectrometry 
      (ToF-SIMS) is widely used for the direct detection of inorganic and organic 
      substances with high spatial resolution but its capability to detect 
      nanoparticles in tissue sections is still insufficiently explored. To estimate 
      the applicability of this technique for nanotoxicological questions, comparative 
      studies with established techniques on the detection of nanoparticles can offer 
      additional insights. Here, we compare ToF-SIMS imaging data with sub-micrometer 
      spatial resolution to fluorescence microscopy imaging data to explore the 
      usefulness of ToF-SIMS for the detection of nanoparticles in tissues. SiO(2) 
      nanoparticles with a mean diameter of 25 nm, core-labelled with fluorescein 
      isothiocyanate, were intratracheally instilled into rat lungs. Subsequently, 
      imaging of lung cryosections was performed with ToF-SIMS and fluorescence 
      microscopy. Nanoparticles were successfully detected with ToF-SIMS in 3D 
      microanalysis mode based on the lateral distribution of SiO(3)(-) (m/z 75.96), 
      which was co-localized with the distribution pattern that was obtained from 
      nanoparticle fluorescence. In addition, the lateral distribution of protein 
      (CN(-), m/z 26.00) and phosphate based signals (PO(3)(-), m/z 78.96) originating 
      from the tissue material could be related to the SiO(3)(-) lateral distribution. 
      In conclusion, ToF-SIMS is suitable to directly detect and laterally resolve 
      SiO(2) nanomaterials in biological tissue at sufficient intensity levels. At the 
      same time, information about the chemical environment of the nanoparticles in the 
      lung tissue sections is obtained.
FAU - Veith, Lothar
AU  - Veith L
AD  - Tascon GmbH, Mendelstrasse 17, D-48149 Munster, Germany. 
      lothar.veith@tascon-gmbh.de.
FAU - Vennemann, Antje
AU  - Vennemann A
FAU - Breitenstein, Daniel
AU  - Breitenstein D
FAU - Engelhard, Carsten
AU  - Engelhard C
FAU - Wiemann, Martin
AU  - Wiemann M
FAU - Hagenhoff, Birgit
AU  - Hagenhoff B
LA  - eng
PT  - Journal Article
PL  - England
TA  - Analyst
JT  - The Analyst
JID - 0372652
RN  - 7631-86-9 (Silicon Dioxide)
SB  - IM
MH  - Animals
MH  - Female
MH  - Lung/*diagnostic imaging
MH  - *Microscopy, Fluorescence
MH  - Nanoparticles/*analysis
MH  - Rats
MH  - Rats, Wistar
MH  - Silicon Dioxide/*analysis
MH  - *Spectrometry, Mass, Secondary Ion
EDAT- 2017/06/14 06:00
MHDA- 2018/11/27 06:00
CRDT- 2017/06/14 06:00
PHST- 2017/06/14 06:00 [pubmed]
PHST- 2018/11/27 06:00 [medline]
PHST- 2017/06/14 06:00 [entrez]
AID - 10.1039/c7an00399d [doi]
PST - ppublish
SO  - Analyst. 2017 Jul 10;142(14):2631-2639. doi: 10.1039/c7an00399d.