PMID- 28609560
OWN - NLM
STAT- MEDLINE
DCOM- 20180608
LR  - 20181029
IS  - 1097-4547 (Electronic)
IS  - 0360-4012 (Linking)
VI  - 95
IP  - 12
DP  - 2017 Dec
TI  - Resolving the contributions of anaesthesia, surgery, and nerve injury on brain 
      derived neurotrophic factor expression in the medial prefrontal cortex of male 
      rats in the CCI model of neuropathic pain.
PG  - 2376-2390
LID - 10.1002/jnr.24095 [doi]
AB  - The medial prefrontal cortex (mPFC) is critical for selecting and shaping complex 
      behavioral responses. In rodent models of neuropathic pain there is evidence for 
      both structural and functional changes in the mPFC. Brain derived neurotrophic 
      factor (BDNF) plays a critical role in the normal functioning of the mPFC. It has 
      been suggested that the disruption of complex behaviors and mood seen in some 
      neuropathic pain patients is mediated in part by alterations of BDNF in this 
      cortical region. In Sprague-Dawley rats, mPFC levels of BDNF and TrkB mRNA and 
      protein, were quantified and compared to controls (n = 24) 6 days after either: 
      (a) halothane (1.5%) anaesthesia (n = 12), (b) sham surgery under halothane 
      (n = 12), (c) sciatic nerve chronic constriction injury under halothane (n = 48). 
      The social behaviors of the rats were quantified daily during the experimental 
      period. Halothane anaesthesia increased BDNF and TrkB mRNA bilaterally. These 
      increases were reversed in rats that underwent sham surgical and nerve injury 
      procedures. Further, halothane anaesthesia, surgical procedures, and nerve injury 
      each decreased BDNF protein levels. These results reveal a marked and distinct 
      BDNF expression profile in the mPFC of rats that have undergone each stage of the 
      procedure to produce neuropathic pain by chronic constriction injury of the 
      sciatic nerve. The highly sensitive nature of neurotrophic signalling to general 
      anaesthesia in the mature neuronal circuit of the adult rat brain highlights the 
      importance of careful evaluation and interpretation of data evaluating the 
      effects of experimental procedures on neural substrates.
CI  - (c) 2017 Wiley Periodicals, Inc.
FAU - Kang, James W M
AU  - Kang JWM
AUID- ORCID: 0000-0002-8517-5656
AD  - Discipline of Anatomy & Histology, School of Medical Sciences, The University of 
      Sydney, New South Wales, 2006, Australia.
AD  - Discipline of Biomedical Sciences, School of Medical Sciences, The University of 
      Sydney, New South Wales, 2006, Australia.
FAU - Keay, Kevin A
AU  - Keay KA
AD  - Discipline of Anatomy & Histology, School of Medical Sciences, The University of 
      Sydney, New South Wales, 2006, Australia.
FAU - Mor, David
AU  - Mor D
AD  - Discipline of Biomedical Sciences, School of Medical Sciences, The University of 
      Sydney, New South Wales, 2006, Australia.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20170613
PL  - United States
TA  - J Neurosci Res
JT  - Journal of neuroscience research
JID - 7600111
RN  - 0 (Anesthetics, Inhalation)
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - UQT9G45D1P (Halothane)
SB  - IM
MH  - Anesthetics, Inhalation/*pharmacology
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/*biosynthesis
MH  - Disease Models, Animal
MH  - Halothane/pharmacology
MH  - Male
MH  - Neuralgia/etiology/*metabolism
MH  - Prefrontal Cortex/*drug effects/*metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Sciatic Nerve/injuries
OTO - NOTNLM
OT  - BDNF
OT  - anaesthesia
OT  - nerve injury
OT  - neuropathic pain
OT  - prefrontal cortex
EDAT- 2017/06/14 06:00
MHDA- 2018/06/09 06:00
CRDT- 2017/06/14 06:00
PHST- 2017/03/22 00:00 [received]
PHST- 2017/05/05 00:00 [revised]
PHST- 2017/05/10 00:00 [accepted]
PHST- 2017/06/14 06:00 [pubmed]
PHST- 2018/06/09 06:00 [medline]
PHST- 2017/06/14 06:00 [entrez]
AID - 10.1002/jnr.24095 [doi]
PST - ppublish
SO  - J Neurosci Res. 2017 Dec;95(12):2376-2390. doi: 10.1002/jnr.24095. Epub 2017 Jun 
      13.