PMID- 28611468
OWN - NLM
STAT- MEDLINE
DCOM- 20190206
LR  - 20240326
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 7
IP  - 1
DP  - 2017 Jun 13
TI  - Insights into enterotoxigenic Escherichia coli diversity in Bangladesh utilizing 
      genomic epidemiology.
PG  - 3402
LID - 10.1038/s41598-017-03631-x [doi]
LID - 3402
AB  - Enterotoxigenic Escherichia coli (ETEC) cause more than 500,000 deaths each year 
      in the developing world and are characterized on a molecular level by the 
      presence of genes that encode the heat-stable (ST) and/or heat-labile (LT) 
      enterotoxins, as well as surface structures, known as colonization factors (CFs). 
      Genome sequencing and comparative genomic analyses of 94 previously 
      uncharacterized ETEC isolates demonstrated remarkable genomic diversity, with 28 
      distinct sequence types identified in three phylogenomic groups. Interestingly, 
      there is a correlation between the genomic sequence type and virulence factor 
      profiles based on prevalence of the isolate, suggesting that there is an optimal 
      combination of genetic factors required for survival, virulence and transmission 
      in the most successful clones. A large-scale BLAST score ratio (LS-BSR) analysis 
      was further applied to identify ETEC-specific genomic regions when compared to 
      non-ETEC genomes, as well as genes that are more associated with clinical 
      presentations or other genotypic markers. Of the strains examined, 21 of 94 ETEC 
      isolates lacked any previously identified CF. Homology searches with the 
      structural subunits of known CFs identified 6 new putative CF variants. These 
      studies provide a roadmap to exploit genomic analyses by directing investigations 
      of pathogenesis, virulence regulation and vaccine development.
FAU - Sahl, Jason W
AU  - Sahl JW
AD  - Institute for Genome Sciences, Department of Microbiology and Immunology, 
      University of Maryland School of Medicine, 801 W. Baltimore Street, Suite 600, 
      Baltimore, MD, 21201, USA.
AD  - Translational Genomics Research Institute, Flagstaff, AZ, 86001, USA.
FAU - Sistrunk, Jeticia R
AU  - Sistrunk JR
AUID- ORCID: 0000-0001-9129-6529
AD  - Institute for Genome Sciences, Department of Microbiology and Immunology, 
      University of Maryland School of Medicine, 801 W. Baltimore Street, Suite 600, 
      Baltimore, MD, 21201, USA.
FAU - Baby, Nabilah Ibnat
AU  - Baby NI
AD  - Centre for Vaccine Sciences, Immunology Laboratory, International Centre Center 
      for Diarrhoeal Disease Research, Mohakhali, Dhaka, 1212, Bangladesh.
FAU - Begum, Yasmin
AU  - Begum Y
AD  - Centre for Vaccine Sciences, Immunology Laboratory, International Centre Center 
      for Diarrhoeal Disease Research, Mohakhali, Dhaka, 1212, Bangladesh.
FAU - Luo, Qingwei
AU  - Luo Q
AD  - Department of Medicine, Division of Infectious Diseases, Washington, USA.
FAU - Sheikh, Alaullah
AU  - Sheikh A
AUID- ORCID: 0000-0002-2972-6978
AD  - Centre for Vaccine Sciences, Immunology Laboratory, International Centre Center 
      for Diarrhoeal Disease Research, Mohakhali, Dhaka, 1212, Bangladesh.
AD  - The Molecular Microbiology and Microbial Pathogenesis Program, Division of 
      Biology and Biomedical Sciences, Washington University in St. Louis, Washington, 
      USA.
FAU - Qadri, Firdausi
AU  - Qadri F
AD  - Centre for Vaccine Sciences, Immunology Laboratory, International Centre Center 
      for Diarrhoeal Disease Research, Mohakhali, Dhaka, 1212, Bangladesh.
FAU - Fleckenstein, James M
AU  - Fleckenstein JM
AD  - Department of Medicine, Division of Infectious Diseases, Washington, USA.
AD  - The Molecular Microbiology and Microbial Pathogenesis Program, Division of 
      Biology and Biomedical Sciences, Washington University in St. Louis, Washington, 
      USA.
AD  - Medicine Service, Veterans Affairs Medical Center, St. Louis, MO, USA.
FAU - Rasko, David A
AU  - Rasko DA
AD  - Institute for Genome Sciences, Department of Microbiology and Immunology, 
      University of Maryland School of Medicine, 801 W. Baltimore Street, Suite 600, 
      Baltimore, MD, 21201, USA. drasko@som.umaryland.edu.
LA  - eng
GR  - I01 BX001469/BX/BLRD VA/United States
GR  - R01 AI089894/AI/NIAID NIH HHS/United States
GR  - R01 AI126887/AI/NIAID NIH HHS/United States
GR  - HHSN272200900009C/AI/NIAID NIH HHS/United States
GR  - U19 AI090873/AI/NIAID NIH HHS/United States
GR  - T32 AI095190/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20170613
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - 0 (DNA, Bacterial)
RN  - 0 (Escherichia coli Proteins)
RN  - 0 (Virulence Factors)
SB  - IM
MH  - Bangladesh/epidemiology
MH  - Case-Control Studies
MH  - DNA, Bacterial
MH  - Diarrhea/epidemiology/*genetics/microbiology
MH  - Enterotoxigenic Escherichia coli/*pathogenicity
MH  - Escherichia coli Infections/epidemiology/*genetics/microbiology
MH  - Escherichia coli Proteins/*genetics
MH  - *Genetic Variation
MH  - Genomics/*methods
MH  - Genotype
MH  - Humans
MH  - Phylogeny
MH  - Virulence Factors/*genetics
PMC - PMC5469772
COIS- The authors declare that they have no competing interests.
EDAT- 2017/06/15 06:00
MHDA- 2019/02/07 06:00
PMCR- 2017/06/13
CRDT- 2017/06/15 06:00
PHST- 2017/01/19 00:00 [received]
PHST- 2017/04/28 00:00 [accepted]
PHST- 2017/06/15 06:00 [entrez]
PHST- 2017/06/15 06:00 [pubmed]
PHST- 2019/02/07 06:00 [medline]
PHST- 2017/06/13 00:00 [pmc-release]
AID - 10.1038/s41598-017-03631-x [pii]
AID - 3631 [pii]
AID - 10.1038/s41598-017-03631-x [doi]
PST - epublish
SO  - Sci Rep. 2017 Jun 13;7(1):3402. doi: 10.1038/s41598-017-03631-x.