PMID- 28611685
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201001
IS  - 1664-042X (Print)
IS  - 1664-042X (Electronic)
IS  - 1664-042X (Linking)
VI  - 8
DP  - 2017
TI  - Entosis Acts as a Novel Way within Sertoli Cells to Eliminate Spermatozoa in 
      Seminiferous Tubule.
PG  - 361
LID - 10.3389/fphys.2017.00361 [doi]
LID - 361
AB  - The present study was designed to investigate the hypothesis that in vivo entosis 
      is a novel pathway for eliminating spermatozoa in the seminiferous tubules (ST) 
      during hibernation of the Chinese soft-shelled turtle. Western blot analysis 
      revealed that the expression of LAMP1 in the testis was significantly higher 
      during hibernation than that during non-hibernation. Immunohistochemistry 
      reaction showed that LAMP1-positive substance was distributed within the Sertoli 
      cells of the testis. Further examination by transmission electron microscopy 
      (TEM), many degraded spermatozoa being enwrapped within large entotic vacuoles in 
      Sertoli cells. The nucleus and the flagellum of the spermatozoa were shown to be 
      decomposed and digested inside entotic vacuoles within Sertoli cells. More than 
      two spermatozoa heads were always observed in each internalized vacuoles. 
      Deserving note is that, a number of different autophagosomes, including initial 
      autophagic vesicles and degradative autophagic vesicles were found inside the 
      entotic vacuoles of the Sertoli cells during hibernation. At the end of 
      hibernation, entotic vacuoles and their autophagosomes disappeared, and numerous 
      large lipid droplets (LDs) appeared within the Sertoli cells. Adherens junctions 
      were apparent between Sertoli cells and developing germ cells, which is the 
      ultrastructural basis of entosis. Taken together, the results presented here show 
      that in the turtle: (1) entosis with internal autophagosomes can take place 
      within normal body cells during hibernation; (2) spermatozoa, as a highly 
      differentiated cell can be internalized and degraded within Sertoli cell by 
      entosis in vivo, which is in favor of the next reproductive cycle in the turtle.
FAU - Ahmed, Nisar
AU  - Ahmed N
AD  - Laboratory of Animal Cell Biology and Embryology, College of Veterinary Medicine, 
      Nanjing Agricultural UniversityNanjing, China.
AD  - Faculty of Veterinary and Animal Sciences, Lasbela University of Agriculture, 
      Water and Marine Sciences (LUAWMS)Uthal, Pakistan.
FAU - Yang, Ping
AU  - Yang P
AD  - Laboratory of Animal Cell Biology and Embryology, College of Veterinary Medicine, 
      Nanjing Agricultural UniversityNanjing, China.
FAU - Huang, Yufei
AU  - Huang Y
AD  - Laboratory of Animal Cell Biology and Embryology, College of Veterinary Medicine, 
      Nanjing Agricultural UniversityNanjing, China.
FAU - Chen, Hong
AU  - Chen H
AD  - Laboratory of Animal Cell Biology and Embryology, College of Veterinary Medicine, 
      Nanjing Agricultural UniversityNanjing, China.
FAU - Liu, Tengfei
AU  - Liu T
AD  - Laboratory of Animal Cell Biology and Embryology, College of Veterinary Medicine, 
      Nanjing Agricultural UniversityNanjing, China.
FAU - Wang, Lingling
AU  - Wang L
AD  - Laboratory of Animal Cell Biology and Embryology, College of Veterinary Medicine, 
      Nanjing Agricultural UniversityNanjing, China.
FAU - Nabi, Fazul
AU  - Nabi F
AD  - Faculty of Veterinary and Animal Sciences, Lasbela University of Agriculture, 
      Water and Marine Sciences (LUAWMS)Uthal, Pakistan.
FAU - Liu, Yi
AU  - Liu Y
AD  - Laboratory of Animal Cell Biology and Embryology, College of Veterinary Medicine, 
      Nanjing Agricultural UniversityNanjing, China.
FAU - Chen, Qiusheng
AU  - Chen Q
AD  - Laboratory of Animal Cell Biology and Embryology, College of Veterinary Medicine, 
      Nanjing Agricultural UniversityNanjing, China.
LA  - eng
PT  - Journal Article
DEP - 20170530
PL  - Switzerland
TA  - Front Physiol
JT  - Frontiers in physiology
JID - 101549006
PMC - PMC5447735
OTO - NOTNLM
OT  - Chinese soft-shelled turtle
OT  - Sertoli cell
OT  - hibernation
OT  - in vivo entosis
OT  - spermatozoa
EDAT- 2017/06/15 06:00
MHDA- 2017/06/15 06:01
PMCR- 2017/05/30
CRDT- 2017/06/15 06:00
PHST- 2017/04/06 00:00 [received]
PHST- 2017/05/16 00:00 [accepted]
PHST- 2017/06/15 06:00 [entrez]
PHST- 2017/06/15 06:00 [pubmed]
PHST- 2017/06/15 06:01 [medline]
PHST- 2017/05/30 00:00 [pmc-release]
AID - 10.3389/fphys.2017.00361 [doi]
PST - epublish
SO  - Front Physiol. 2017 May 30;8:361. doi: 10.3389/fphys.2017.00361. eCollection 
      2017.