PMID- 28612046
OWN - NLM
STAT- MEDLINE
DCOM- 20180328
LR  - 20181113
IS  - 2373-2822 (Electronic)
IS  - 2373-2822 (Linking)
VI  - 4
IP  - 3
DP  - 2017 May-Jun
TI  - Shaping of Signal Transmission at the Photoreceptor Synapse by EAAT2 Glutamate 
      Transporters.
LID - 10.1523/ENEURO.0339-16.2017 [doi]
LID - ENEURO.0339-16.2017
AB  - Photoreceptor ribbon synapses tonically release glutamate. To ensure efficient 
      signal transmission and prevent glutamate toxicity, a highly efficient glutamate 
      removal system provided by members of the SLC1 gene family is required. By using 
      a combination of biophysical and in vivo studies, we elucidate the role of 
      excitatory amino acid transporter 2 (EAAT2) proteins in synaptic glutamate 
      homeostasis at the zebrafish photoreceptor synapse. The main glutamate sink is 
      provided by the glial EAAT2a, reflected by reduced electroretinographic responses 
      in EAAT2a-depleted larvae. EAAT2b is located on the tips of cone pedicles and 
      contributes little to glutamate reuptake. However, this transporter displays both 
      a large chloride conductance and leak current, being important in stabilizing the 
      cone resting potential. This work demonstrates not only how proteins originating 
      from the same gene family can complement each other's expression profiles and 
      biophysical properties, but also how presynaptic and glial transporters are 
      coordinated to ensure efficient synaptic transmission at glutamatergic synapses 
      of the central nervous system.
FAU - Niklaus, Stephanie
AU  - Niklaus S
AD  - Institute of Molecular Life Sciences, University of Zurich, Zurich, CH-8057, 
      Switzerland.
AD  - Life Science Zurich Graduate Program - Neuroscience, Zurich, CH-8057, 
      Switzerland.
FAU - Cadetti, Lucia
AU  - Cadetti L
AD  - Institute of Molecular Life Sciences, University of Zurich, Zurich, CH-8057, 
      Switzerland.
FAU - Vom Berg-Maurer, Colette M
AU  - Vom Berg-Maurer CM
AUID- ORCID: 0000-0002-8870-9799
AD  - Institute of Molecular Life Sciences, University of Zurich, Zurich, CH-8057, 
      Switzerland.
FAU - Lehnherr, Andre
AU  - Lehnherr A
AUID- ORCID: 0000-0001-8201-3590
AD  - Institute of Molecular Life Sciences, University of Zurich, Zurich, CH-8057, 
      Switzerland.
AD  - Life Science Zurich Graduate Program - Neuroscience, Zurich, CH-8057, 
      Switzerland.
FAU - Hotz, Adriana L
AU  - Hotz AL
AD  - Institute of Molecular Life Sciences, University of Zurich, Zurich, CH-8057, 
      Switzerland.
FAU - Forster, Ian C
AU  - Forster IC
AD  - Institute of Physiology, Zurich, CH-8057, Switzerland.
FAU - Gesemann, Matthias
AU  - Gesemann M
AD  - Institute of Molecular Life Sciences, University of Zurich, Zurich, CH-8057, 
      Switzerland.
FAU - Neuhauss, Stephan C F
AU  - Neuhauss SCF
AUID- ORCID: 0000-0002-9615-480X
AD  - Institute of Molecular Life Sciences, University of Zurich, Zurich, CH-8057, 
      Switzerland.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20170612
PL  - United States
TA  - eNeuro
JT  - eNeuro
JID - 101647362
RN  - 0 (Excitatory Amino Acid Transporter 2)
RN  - 0 (Morpholinos)
RN  - 3KX376GY7L (Glutamic Acid)
SB  - IM
MH  - Animals
MH  - Animals, Genetically Modified
MH  - Excitatory Amino Acid Transporter 2/genetics/*metabolism
MH  - Eye/cytology
MH  - Gene Expression Regulation/genetics
MH  - Glutamic Acid/metabolism
MH  - In Vitro Techniques
MH  - Larva
MH  - Morpholinos/genetics/metabolism
MH  - Oocytes/physiology
MH  - Patch-Clamp Techniques
MH  - Photoreceptor Cells/*classification/*metabolism
MH  - Presynaptic Terminals/*metabolism
MH  - Retina/anatomy & histology
MH  - Signal Transduction/drug effects/genetics
MH  - Synapses
MH  - Synaptic Transmission/physiology
MH  - Visual Pathways/physiology
MH  - Xenopus
MH  - Zebrafish
PMC - PMC5467398
OTO - NOTNLM
OT  - Excitatory amino acid transporter
OT  - glutamate
OT  - retina
OT  - zebrafish
COIS- Authors report no conflict of interest.
EDAT- 2017/06/15 06:00
MHDA- 2018/03/29 06:00
PMCR- 2017/06/12
CRDT- 2017/06/15 06:00
PHST- 2016/11/10 00:00 [received]
PHST- 2017/05/12 00:00 [revised]
PHST- 2017/05/25 00:00 [accepted]
PHST- 2017/06/15 06:00 [entrez]
PHST- 2017/06/15 06:00 [pubmed]
PHST- 2018/03/29 06:00 [medline]
PHST- 2017/06/12 00:00 [pmc-release]
AID - eN-NWR-0339-16 [pii]
AID - 10.1523/ENEURO.0339-16.2017 [doi]
PST - epublish
SO  - eNeuro. 2017 Jun 12;4(3):ENEURO.0339-16.2017. doi: 10.1523/ENEURO.0339-16.2017. 
      eCollection 2017 May-Jun.