PMID- 28612523
OWN - NLM
STAT- MEDLINE
DCOM- 20190528
LR  - 20190528
IS  - 1672-173X (Print)
IS  - 1672-173X (Linking)
VI  - 48
IP  - 2
DP  - 2017 Mar
TI  - [Protective Effects of Intraventricular Transplanted Human Umbilical Cord-derived 
      Mesenchymal Stem Cells on Hypoxic Ischemic Brain Damages in Rats].
PG  - 179-185
AB  - OBJECTIVES: To determine the therapeutic effect and protective mechanism of human 
      umbilical cord-derived mesenchymal stem cells (hUC-MSCs) on newborn rats with 
      hypoxia ischemic brain damage (HIBD). METHODS: Umbilical cord (3-4 cm) was 
      collected from a healthy male infant for preparation of hUC-MSCs using explants 
      technique. The hUC-MSCs were cultured and labeled with BrdU. The differentiation 
      function of MSCs was identified. Healthy SPF grade neonatal SD rats were randomly 
      divided into sham (n =30), HIBD (n =36) and hUC-MSCs treated HIBD (n =32) groups. 
      BrdU-labeled hUC-MSCs were injected into the right ventricle of the rats in the 
      hUC-MSCs treatment group 24 h after successful induction of HIBD. The growth and 
      development of the rats were recorded. The neurological behavior of the rats were 
      evaluated with Longa score method 3 weeks after hUC-MSCs transplantation. The 
      survival, migration, differentiation and pro-differentiation of the transplanted 
      hUC-MSCs were measured using immunological fluorescence method. RESULTS: Rats in 
      the hUC-MSCs treatment group had significant higher weight gain and lower Longa 
      scores (at the second and the third week post transplantation) than those in the 
      HIBD group (P<0.05). BrdU positive cells were found in brain tissues 3 weeks 
      after transplantation, and they were mainly distributed in the damaged 
      hippocampus and cerebral cortex. Three weeks after transplantation, the total 
      signal strength of glial fibrillary acidic protein (GFAP) or neuron-specific 
      enclase (NSE) gradually increased. CONCLUSION: Transplanted hUC-MSCs can migrate 
      brain damages through differentiating into neuron-like cells and promoting 
      endogenous neurological differentiations.
FAU - Fan, Xue
AU  - Fan X
AD  - Department of Neonatology, West China Second University Hospital, Sichuan 
      University, Chengdu 610041, China.
AD  - Key Laboratory of Birth Defects and Related Diseases of Women and Children 
      (Sichuan University), Ministry of Education, Chengdu 610041, China.
FAU - Bai, Xiao-Hong
AU  - Bai XH
AD  - Department of Neonatology, West China Second University Hospital, Sichuan 
      University, Chengdu 610041, China.
AD  - Key Laboratory of Birth Defects and Related Diseases of Women and Children 
      (Sichuan University), Ministry of Education, Chengdu 610041, China.
FAU - Chen, Juan
AU  - Chen J
AD  - Department of Neonatology, West China Second University Hospital, Sichuan 
      University, Chengdu 610041, China.
AD  - Key Laboratory of Birth Defects and Related Diseases of Women and Children 
      (Sichuan University), Ministry of Education, Chengdu 610041, China.
FAU - Wan, Qian
AU  - Wan Q
AD  - Sichuan Cord Blood Bank/ Sichuan Neo-Life Stem Cell Biotech Inc.
FAU - Chen, Qiang
AU  - Chen Q
AD  - Sichuan Cord Blood Bank/ Sichuan Neo-Life Stem Cell Biotech Inc.
FAU - Mu, De-Zhi
AU  - Mu DZ
AD  - Department of Neonatology, West China Second University Hospital, Sichuan 
      University, Chengdu 610041, China.
AD  - Key Laboratory of Birth Defects and Related Diseases of Women and Children 
      (Sichuan University), Ministry of Education, Chengdu 610041, China.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Sichuan Da Xue Xue Bao Yi Xue Ban
JT  - Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical 
      science edition
JID - 101162609
SB  - IM
MH  - Animals
MH  - Animals, Newborn
MH  - Cell Differentiation
MH  - Humans
MH  - Hypoxia-Ischemia, Brain/*therapy
MH  - Male
MH  - *Mesenchymal Stem Cell Transplantation
MH  - Mesenchymal Stem Cells/*cytology
MH  - Random Allocation
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Umbilical Cord/*cytology
OTO - NOTNLM
OT  - Brain protection effect
OT  - Cellular transplantation therapy
OT  - Human umbilical cord-derived mesenchymal stem cells (hUC-MSCs)
OT  - Hypoxia ischemic brain damage (HIBD)
EDAT- 2017/06/15 06:00
MHDA- 2019/05/29 06:00
CRDT- 2017/06/15 06:00
PHST- 2017/06/15 06:00 [entrez]
PHST- 2017/06/15 06:00 [pubmed]
PHST- 2019/05/29 06:00 [medline]
PST - ppublish
SO  - Sichuan Da Xue Xue Bao Yi Xue Ban. 2017 Mar;48(2):179-185.