PMID- 28613152
OWN - NLM
STAT- MEDLINE
DCOM- 20170717
LR  - 20211204
IS  - 1465-2099 (Electronic)
IS  - 0022-1317 (Print)
IS  - 0022-1317 (Linking)
VI  - 98
IP  - 6
DP  - 2017 Jun
TI  - Reduction of infection by inhibiting mTOR pathway is associated with reversed 
      repression of type I interferon by porcine reproductive and respiratory syndrome 
      virus.
PG  - 1316-1328
LID - 10.1099/jgv.0.000802 [doi]
AB  - Type I interferons (IFNs) are critical in animal antiviral regulation. 
      IFN-mediated signalling regulates hundreds of genes that are directly associated 
      with antiviral, immune and other physiological responses. The signalling pathway 
      mediated by mechanistic target of rapamycin (mTOR), a serine/threonine kinase 
      regulated by IFNs, is key in regulation of cellular metabolism and was recently 
      implicated in host antiviral responses. However, little is known about how animal 
      type I IFN signalling coordinates immunometabolic reactions during antiviral 
      defence. Here, using porcine reproductive and respiratory syndrome virus (PRRSV), 
      we found that the genes in the mTOR signalling pathway were differently regulated 
      in PRRSV-infected porcine alveolar macrophages at different activation statuses. 
      Moreover, mTOR signalling regulated PRRSV infection in MARC-145 and primary 
      porcine cells, in part, through modulating the production and signalling of type 
      I IFNs. Taken together, we determined that the mTOR signalling pathway involves 
      PRRSV infection and regulates expression and signalling of type I IFNs against 
      viral infection. These findings suggest that the mTOR signalling pathway has a 
      bi-directional loop with the type I IFN system and imply that some components in 
      the mTOR signalling pathway can be utilized as targets for studying antiviral 
      immunity and for designing therapeutic reagents.
FAU - Liu, Qinfang
AU  - Liu Q
AD  - Departments of Anatomy and Physiology, College of Veterinary Medicine, Kansas 
      State University, Manhattan, KS, USA.
FAU - Miller, Laura C
AU  - Miller LC
AD  - USDA, Agricultural Research Service, National Animal Disease Center, Virus and 
      Prion Research Unit, 1920 Dayton Avenue, Ames, IA 50010, USA.
FAU - Blecha, Frank
AU  - Blecha F
AD  - Departments of Anatomy and Physiology, College of Veterinary Medicine, Kansas 
      State University, Manhattan, KS, USA.
FAU - Sang, Yongming
AU  - Sang Y
AD  - Present address: Department of Agricultural and Environmental Sciences, College 
      of Agriculture, Human and Natural Sciences, Tennessee State University, 3500 John 
      A. Merritt Boulevard, Nashville, TN, USA.
AD  - Departments of Anatomy and Physiology, College of Veterinary Medicine, Kansas 
      State University, Manhattan, KS, USA.
LA  - eng
GR  - P20 RR017686/RR/NCRR NIH HHS/United States
PT  - Journal Article
DEP - 20170614
PL  - England
TA  - J Gen Virol
JT  - The Journal of general virology
JID - 0077340
RN  - 0 (Interferon Type I)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
SB  - IM
MH  - Animals
MH  - Cells, Cultured
MH  - Epithelial Cells/immunology/virology
MH  - *Host-Pathogen Interactions
MH  - Interferon Type I/*metabolism
MH  - Macrophages, Alveolar/immunology/virology
MH  - Porcine respiratory and reproductive syndrome virus/*physiology
MH  - Signal Transduction
MH  - Swine
MH  - TOR Serine-Threonine Kinases/*antagonists & inhibitors
PMC - PMC5962895
COIS- The authors declare that there are no conflicts of interest.
EDAT- 2017/06/15 06:00
MHDA- 2017/07/18 06:00
PMCR- 2017/06/14
CRDT- 2017/06/15 06:00
PHST- 2017/06/15 06:00 [pubmed]
PHST- 2017/07/18 06:00 [medline]
PHST- 2017/06/15 06:00 [entrez]
PHST- 2017/06/14 00:00 [pmc-release]
AID - 000802 [pii]
AID - 10.1099/jgv.0.000802 [doi]
PST - ppublish
SO  - J Gen Virol. 2017 Jun;98(6):1316-1328. doi: 10.1099/jgv.0.000802. Epub 2017 Jun 
      14.