PMID- 28614607
OWN - NLM
STAT- MEDLINE
DCOM- 20180423
LR  - 20181128
IS  - 1365-2826 (Electronic)
IS  - 0953-8194 (Linking)
VI  - 29
IP  - 7
DP  - 2017 Jul
TI  - Anatomical and functional implications of corticotrophin-releasing hormone 
      neurones in a septal nucleus of the avian brain: an emphasis on glial-neuronal 
      interaction via V1a receptors in vitro.
LID - 10.1111/jne.12494 [doi]
AB  - Previously, we showed that corticotrophin-releasing hormone immunoreactive 
      (CRH-IR) neurones in a septal structure are associated with stress and the 
      hypothalamic-pituitary-adrenal axis in birds. In the present study, we focused 
      upon CRH-IR neurones located within the septal structure called the nucleus of 
      the hippocampal commissure (NHpC). Immunocytochemical and gene expression 
      analyses were used to identify the anatomical and functional characteristics of 
      cells within the NHpC. A comparative morphometry analysis showed that CRH-IR 
      neurones in the NHpC were significantly larger than CRH-IR parvocellular neurones 
      in the paraventricular nucleus of the hypothalamus (PVN) and lateral bed nucleus 
      of the stria terminalis. Furthermore, these large neurones in the NHpC usually 
      have more than two processes, showing characteristics of multipolar neurones. 
      Utilisation of an organotypic slice culture method enabled testing of how CRH-IR 
      neurones could be regulated within the NHpC. Similar to the PVN, CRH mRNA levels 
      in the NHpC were increased following forskolin treatment. However, dexamethasone 
      decreased forskolin-induced CRH gene expression only in the PVN and not in the 
      NHpC, indicating differential inhibitory mechanisms in the PVN and the NHpC of 
      the avian brain. Moreover, immunocytochemical evidence also showed that CRH-IR 
      neurones reside in the NHpC along with the vasotocinergic system, comprising 
      arginine vasotocin (AVT) nerve terminals and immunoreactive vasotocin V1a 
      receptors (V1aR) in glia. Hence, we hypothesised that AVT acts as a 
      neuromodulator within the NHpC to modulate activity of CRH neurones via glial 
      V1aR. Gene expression analysis of cultured slices revealed that AVT treatment 
      increased CRH mRNA levels, whereas a combination of AVT and a V1aR antagonist 
      treatment decreased CRH mRNA expression. Furthermore, an attempt to identify an 
      intercellular mechanism in glial-neuronal communication in the NHpC revealed that 
      brain-derived neurotrophic factor (BDNF) and its receptor (TrkB) could be 
      involved in the signalling mechanism. Immunocytochemical results further showed 
      that both BDNF and TrkB receptors were found in glia of the NHpC. Interestingly, 
      in cultured brain slices containing the NHpC, the use of a selective TrkB 
      antagonist decreased the AVT-induced increase in CRH gene expression levels. The 
      results from the present study collectively suggest that CRH neuronal activity is 
      modulated by AVT via V1aR involving BDNF and TrkB glia in the NHpC.
CI  - (c) 2017 British Society for Neuroendocrinology.
FAU - Nagarajan, G
AU  - Nagarajan G
AD  - The Center of Excellence for Poultry Science, University of Arkansas, 
      Fayetteville, AR, USA.
FAU - Jurkevich, A
AU  - Jurkevich A
AD  - Molecular Cytology Research Core Facility, University of Missouri, Columbia, MO, 
      USA.
FAU - Kang, S W
AU  - Kang SW
AD  - The Center of Excellence for Poultry Science, University of Arkansas, 
      Fayetteville, AR, USA.
FAU - Kuenzel, W J
AU  - Kuenzel WJ
AUID- ORCID: 0000-0001-8312-4153
AD  - The Center of Excellence for Poultry Science, University of Arkansas, 
      Fayetteville, AR, USA.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Neuroendocrinol
JT  - Journal of neuroendocrinology
JID - 8913461
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Receptors, Vasopressin)
RN  - 1F7A44V6OU (Colforsin)
RN  - 7S5I7G3JQL (Dexamethasone)
RN  - 9015-71-8 (Corticotropin-Releasing Hormone)
RN  - EC 2.7.10.1 (Receptor, trkB)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/metabolism
MH  - Chickens
MH  - Colforsin/pharmacology
MH  - Corticotropin-Releasing Hormone/*metabolism
MH  - Dexamethasone/pharmacology
MH  - Gene Expression/drug effects
MH  - Hypothalamo-Hypophyseal System/metabolism
MH  - Male
MH  - Neuroglia/*metabolism
MH  - Neurons/*metabolism
MH  - Pituitary-Adrenal System/metabolism
MH  - Receptor, trkB/metabolism
MH  - Receptors, Vasopressin/*metabolism
MH  - Septal Nuclei/*metabolism
OTO - NOTNLM
OT  - BDNF
OT  - CRH
OT  - TrkB
OT  - neurone-glia
OT  - receptor
OT  - vasotocin
EDAT- 2017/06/15 06:00
MHDA- 2018/04/24 06:00
CRDT- 2017/06/15 06:00
PHST- 2017/03/30 00:00 [received]
PHST- 2017/05/31 00:00 [revised]
PHST- 2017/06/09 00:00 [accepted]
PHST- 2017/06/15 06:00 [pubmed]
PHST- 2018/04/24 06:00 [medline]
PHST- 2017/06/15 06:00 [entrez]
AID - 10.1111/jne.12494 [doi]
PST - ppublish
SO  - J Neuroendocrinol. 2017 Jul;29(7). doi: 10.1111/jne.12494.