PMID- 28614774
OWN - NLM
STAT- MEDLINE
DCOM- 20180320
LR  - 20220409
IS  - 1899-1505 (Electronic)
IS  - 0867-5910 (Linking)
VI  - 68
IP  - 2
DP  - 2017 Apr
TI  - New insight into the direct anti-inflammatory activity of a myokine irisin 
      against proinflammatory activation of adipocytes. Implication for exercise in 
      obesity.
PG  - 243-251
AB  - A biological activity of myokine irisin, has been intensively investigated in the 
      context of a browning process occurring in white adipose tissue, but its role as 
      a modulator of immune response has been little studied. The aim of our study was 
      to determine the impact of irisin (0 - 100 nM) on pro-inflammatory activation of 
      adipocyte 3T3 L1 cell line. Irisin reduced in a concentration-dependent manner 
      the expression and activity of major proinflammatory cytokines, e.g. tumor 
      necrosis factor-alpha (TNF-alpha) and interleukin 6 (IL-6) expression and their 
      secretion into cell medium. Moreover, irisin enhanced adiponectin synthesis 
      reversing the effect of the lipopolysaccharide (LPS)-induced attenuation of this 
      adipokine expression. The opposite effect was observed for leptin whose 
      expression increased by LPS and this effect was suppressed by irisin application. 
      A decreased phosphorylation and activation of nuclear factor kappa B (NFkappaB) in 
      the presence of irisin suggests that mechanism of action irisin involves the 
      inhibition of an inflammatory transcription factor. Irisin exerts also an 
      inhibitory effect on macrophage migration toward chemoattractants present in 
      adipocyte supernatants. Among the specific molecules secreted by adipocytes was 
      monocyte chemotactic protein 1 (MCP-1) whose expression was suppressed by irisn. 
      In majority of experiments irisin was effective in 100 nM concentration but in 
      some of them the inhibitory effects occurred already in a concentration of 50 nM 
      of this peptide. This study for the first time showed that adipocytes are 
      directly affected by irisin and provides an evidence on anti-inflammatory action 
      of irisin on fat cells.
FAU - Mazur-Bialy, A I
AU  - Mazur-Bialy AI
AD  - Department of Ergonomics and Exercise Physiology, Faculty of Health Science, 
      Jagiellonian University Medical College, Cracow, Poland. 
      agnieszka.mazur@uj.edu.pl.
FAU - Bilski, J
AU  - Bilski J
AD  - Department of Ergonomics and Exercise Physiology, Faculty of Health Science, 
      Jagiellonian University Medical College, Cracow, Poland.
FAU - Pochec, E
AU  - Pochec E
AD  - Department of Glycoconjugate Biochemistry, Institute of Zoology, Jagiellonian 
      University, Cracow, Poland.
FAU - Brzozowski, T
AU  - Brzozowski T
AD  - Department of Physiology, Faculty of Medicine, Jagiellonian University Medical 
      College, Cracow, Poland.
LA  - eng
PT  - Journal Article
PL  - Poland
TA  - J Physiol Pharmacol
JT  - Journal of physiology and pharmacology : an official journal of the Polish 
      Physiological Society
JID - 9114501
RN  - 0 (Cytokines)
RN  - 0 (FNDC5 protein, mouse)
RN  - 0 (Fibronectins)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (NF-kappa B)
RN  - 0 (PPAR gamma)
SB  - IM
MH  - 3T3-L1 Cells
MH  - Adipocytes/*metabolism
MH  - Animals
MH  - Cell Survival
MH  - Chemotaxis
MH  - Cytokines/genetics/metabolism
MH  - Exercise
MH  - Fibronectins/*metabolism
MH  - Humans
MH  - Inflammation/genetics/*metabolism
MH  - Lipopolysaccharides
MH  - Mice
MH  - NF-kappa B/metabolism
MH  - Obesity
MH  - PPAR gamma/genetics/metabolism
MH  - RAW 264.7 Cells
EDAT- 2017/06/15 06:00
MHDA- 2018/03/21 06:00
CRDT- 2017/06/15 06:00
PHST- 2017/02/20 00:00 [received]
PHST- 2017/04/24 00:00 [accepted]
PHST- 2017/06/15 06:00 [entrez]
PHST- 2017/06/15 06:00 [pubmed]
PHST- 2018/03/21 06:00 [medline]
PST - ppublish
SO  - J Physiol Pharmacol. 2017 Apr;68(2):243-251.