PMID- 28620393
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20191120
IS  - 1664-3224 (Print)
IS  - 1664-3224 (Electronic)
IS  - 1664-3224 (Linking)
VI  - 8
DP  - 2017
TI  - TWEAK/Fn14 Activation Participates in Ro52-Mediated Photosensitization in 
      Cutaneous Lupus Erythematosus.
PG  - 651
LID - 10.3389/fimmu.2017.00651 [doi]
LID - 651
AB  - Tumor necrosis factor (TNF)-like weak inducer of apoptosis (TWEAK) binds to its 
      sole receptor fibroblast growth factor-inducible 14 (Fn14), participating in 
      various inflammatory responses. Recently, TWEAK/Fn14 activation was found 
      prominent in the lesions of cutaneous lupus erythematosus (CLE). This study was 
      designed to further reveal the potential role of this pathway in Ro52-mediated 
      photosensitization. TWEAK, Fn14, and Ro52 were determined in the skin lesions of 
      patients with CLE. Murine keratinocytes received ultraviolet B (UVB) irradiation 
      or plus TWEAK stimulation and underwent detection for Ro52 and proinflammatory 
      cytokines. The chemotaxis of J774.2 macrophages was evaluated on TWEAK 
      stimulation of cocultured keratinocytes. We found that TWEAK, Fn14, and 
      downstream cytokines were highly expressed in CLE lesions that overexpressed 
      Ro52. Moreover, TWEAK enhanced the UVB-induced Ro52 upregulation in murine 
      keratinocytes. Meanwhile, TWEAK stimulation of keratinocytes favored the 
      migration of macrophages through promoting the production of chemokine C-C motif 
      ligands 17 and 22. Furthermore, Fn14 siRNA transfection or nuclear factor-kappa B 
      (NF-kappaB) inhibitor abrogated the TWEAK enhancement of Ro52 expression in 
      keratinocytes. Similarly, TNF receptor associated factor 2 (TRAF2) siRNA reduced 
      the protein level of Ro52 in these cells upon TWEAK stimulation. Interestingly, 
      UVB irradiation increased the expression of TNF receptor type 1 (TNFR1) but not 
      affecting TNFR2 expression in keratinocytes. In conclusion, the TWEAK/Fn14 
      signaling participates in Ro52-mediated photosensitization and involves the 
      activation of NF-kappaB pathway as well as the function of the TRAF2/TNFR partners.
FAU - Liu, Yale
AU  - Liu Y
AD  - Department of Dermatology, The Second Affiliated Hospital, School of Medicine, 
      Xi'an Jiaotong University, Xi'an, China.
FAU - Xu, Meifeng
AU  - Xu M
AD  - Department of Dermatology, The Second Affiliated Hospital, School of Medicine, 
      Xi'an Jiaotong University, Xi'an, China.
FAU - Min, Xiaoyun
AU  - Min X
AD  - Core Research Laboratory, The Second Affiliated Hospital, School of Medicine, 
      Xi'an Jiaotong University, Xi'an, China.
FAU - Wu, Kunyi
AU  - Wu K
AD  - Core Research Laboratory, The Second Affiliated Hospital, School of Medicine, 
      Xi'an Jiaotong University, Xi'an, China.
FAU - Zhang, Ting
AU  - Zhang T
AD  - Core Research Laboratory, The Second Affiliated Hospital, School of Medicine, 
      Xi'an Jiaotong University, Xi'an, China.
FAU - Li, Ke
AU  - Li K
AD  - Core Research Laboratory, The Second Affiliated Hospital, School of Medicine, 
      Xi'an Jiaotong University, Xi'an, China.
FAU - Xiao, Shengxiang
AU  - Xiao S
AD  - Department of Dermatology, The Second Affiliated Hospital, School of Medicine, 
      Xi'an Jiaotong University, Xi'an, China.
FAU - Xia, Yumin
AU  - Xia Y
AD  - Department of Dermatology, The Second Affiliated Hospital, School of Medicine, 
      Xi'an Jiaotong University, Xi'an, China.
LA  - eng
PT  - Journal Article
DEP - 20170531
PL  - Switzerland
TA  - Front Immunol
JT  - Frontiers in immunology
JID - 101560960
PMC - PMC5449764
OTO - NOTNLM
OT  - Fn14
OT  - Ro52
OT  - TWEAK
OT  - cutaneous lupus erythematosus
OT  - keratinocyte
OT  - photosensitization
OT  - tumor necrosis factor receptor
OT  - ultraviolet B
EDAT- 2017/06/18 06:00
MHDA- 2017/06/18 06:01
PMCR- 2017/01/01
CRDT- 2017/06/17 06:00
PHST- 2017/02/27 00:00 [received]
PHST- 2017/05/17 00:00 [accepted]
PHST- 2017/06/17 06:00 [entrez]
PHST- 2017/06/18 06:00 [pubmed]
PHST- 2017/06/18 06:01 [medline]
PHST- 2017/01/01 00:00 [pmc-release]
AID - 10.3389/fimmu.2017.00651 [doi]
PST - epublish
SO  - Front Immunol. 2017 May 31;8:651. doi: 10.3389/fimmu.2017.00651. eCollection 
      2017.