PMID- 28621565
OWN - NLM
STAT- MEDLINE
DCOM- 20180910
LR  - 20180910
IS  - 1557-8550 (Electronic)
IS  - 1549-5418 (Linking)
VI  - 35
IP  - 10
DP  - 2017 Oct
TI  - Light-Emitting Diode at 460 +/- 20 nm Increases the Production of IL-12 and IL-6 in 
      Murine Dendritic Cells.
PG  - 560-566
LID - 10.1089/pho.2016.4244 [doi]
AB  - BACKGROUND DATA: Light emitting diode (LED) therapy has been proposed as an 
      option for the treatment of many skin inflammatory processes. Dendritic cells 
      (DCs) are important cells of skin that participate in the initiation and 
      activation of skin immunity. The modulation of these cells by LED could explain 
      much of its effects. OBJECTIVE: Thus, the aim of this study was to examine the 
      effects of LED at 460 +/- 20 nm on cytokine production and the expression of 
      surface markers on DCs. MATERIALS AND METHODS: DCs were obtained from mouse bone 
      marrow-derived dendritic cells (BMDCs). The LED was applied giving a fluence of 
      3.3, 8.2, or 16.5 J/cm(2) on BMDCs or lipopolysaccharide (LPS)-matured BMDCs. The 
      production of cytokine was analyzed by enzyme linked immunosorbant assay (ELISA) 
      and the expression of DC co- and stimulatory was analyzed markers by cytometry. 
      RESULTS: LED increases IL-12p40 and IL-6 production in both nonstimulated BMDCs 
      and LPS-matured BMDCs. The expression of MHC-II molecule was inhibited and the 
      expression of the CD86 molecule was increased in nonstimulated BMDCs but not in 
      LPS-matured BMDCs. The production of tumor necrosis factor alpha (TNF-alpha) and 
      interleukin-10 (IL-10) and the expression of CD40 were not altered. CONCLUSIONS: 
      These results demonstrate that LED stimulated cytokine production in BMDCs, 
      suggesting a proinflammatory role in the tested conditions and maybe it can 
      increase DC maturation.
FAU - Carvalho-Costa, Tamires Marielem
AU  - Carvalho-Costa TM
AD  - 1 Laboratory of Immunology, Federal University of Triangulo Mineiro , Uberaba, 
      Brazil .
FAU - Mendes, Maria Tays
AU  - Mendes MT
AD  - 1 Laboratory of Immunology, Federal University of Triangulo Mineiro , Uberaba, 
      Brazil .
FAU - da Silva, Marcos Vinicius
AU  - da Silva MV
AD  - 1 Laboratory of Immunology, Federal University of Triangulo Mineiro , Uberaba, 
      Brazil .
FAU - Rodrigues, Virmondes Jr
AU  - Rodrigues V Jr
AD  - 1 Laboratory of Immunology, Federal University of Triangulo Mineiro , Uberaba, 
      Brazil .
FAU - Bruschi Thedei, Giuliana Cristina Marre
AU  - Bruschi Thedei GCM
AD  - 2 Laboratory of Molecular Biology, University of Uberaba , Uberaba, Brazil .
FAU - Oliveira, Carlo Jose Freire
AU  - Oliveira CJF
AD  - 1 Laboratory of Immunology, Federal University of Triangulo Mineiro , Uberaba, 
      Brazil .
FAU - Thedei, Geraldo Jr
AU  - Thedei G Jr
AD  - 2 Laboratory of Molecular Biology, University of Uberaba , Uberaba, Brazil .
LA  - eng
PT  - Journal Article
DEP - 20170615
PL  - United States
TA  - Photomed Laser Surg
JT  - Photomedicine and laser surgery
JID - 101222340
RN  - 0 (Cytokines)
RN  - 0 (Interleukin-6)
RN  - 187348-17-0 (Interleukin-12)
SB  - IM
MH  - Analysis of Variance
MH  - Animals
MH  - Cell Differentiation/radiation effects
MH  - Cells, Cultured
MH  - Cytokines/metabolism/radiation effects
MH  - Dendritic Cells/metabolism/*radiation effects
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Interleukin-12/*metabolism/radiation effects
MH  - Interleukin-6/*metabolism/radiation effects
MH  - *Lasers, Semiconductor
MH  - Mice
MH  - Mice, Inbred C57BL
OTO - NOTNLM
OT  - immunomodulation
OT  - light-emitting diode
OT  - photobiomodulation
EDAT- 2017/06/18 06:00
MHDA- 2018/09/11 06:00
CRDT- 2017/06/17 06:00
PHST- 2017/06/18 06:00 [pubmed]
PHST- 2018/09/11 06:00 [medline]
PHST- 2017/06/17 06:00 [entrez]
AID - 10.1089/pho.2016.4244 [doi]
PST - ppublish
SO  - Photomed Laser Surg. 2017 Oct;35(10):560-566. doi: 10.1089/pho.2016.4244. Epub 
      2017 Jun 15.