PMID- 28621903
OWN - NLM
STAT- MEDLINE
DCOM- 20171226
LR  - 20220311
IS  - 2042-3306 (Electronic)
IS  - 0425-1644 (Linking)
VI  - 50
IP  - 1
DP  - 2018 Jan
TI  - Histological assessment of beta-amyloid precursor protein immunolabelled rectal 
      biopsies aids diagnosis of equine grass sickness.
PG  - 22-28
LID - 10.1111/evj.12710 [doi]
AB  - BACKGROUND: An accurate, minimally invasive, ante-mortem diagnostic test for 
      equine grass sickness (EGS) is currently lacking. Although histological 
      examination of haematoxylin and eosin-stained rectal biopsies for chromatolytic 
      neurons is insensitive as a diagnostic test for EGS, we hypothesised that its 
      diagnostic accuracy could be improved by immunolabelling for beta-amyloid precursor 
      protein (beta-APP), which has increased expression in cranial cervical ganglia (CCG) 
      neuronal perikarya in EGS. OBJECTIVES: To develop a grading scheme for assessing 
      the distribution and intensity of beta-APP immunoreactivity within individual rectal 
      submucosal neurons and subsequently to determine the value of the distribution of 
      different grades of neurons in EGS diagnosis. STUDY DESIGN: Retrospective 
      case-control diagnostic accuracy study. METHODS: Initially, a standardised 
      grading scheme was developed and beta-APP immunoreactivity in individual neuronal 
      perikarya and axons was compared in sections of CCG and ileum from EGS and 
      control horses. The grading scheme was then refined before being blindly applied 
      to submucosal neurons in rectal biopsies derived from 21 EGS and 23 control 
      horses. RESULTS: beta-APP immunoreactivity was increased in neuronal perikarya and 
      axons in sections of CCG, ileum and rectum from EGS horses compared with 
      controls. For rectal biopsies, a mean immunoreactivity grade exceeding 1.1 was 
      100% specific and sensitive for EGS, and the presence of at least one neuron with 
      diffuse labelling of the entire cytoplasm (grade 3) was 95% sensitive and 100% 
      specific for EGS. MAIN LIMITATIONS: Although the diagnostic criteria facilitated 
      the discrimination of the EGS and control biopsies evaluated in this study, 
      further prospective validation using a larger sample set is required. 
      CONCLUSIONS: Histological assessment of beta-APP immunolabelled rectal biopsies is 
      more sensitive than conventional histological examination in EGS diagnosis. 
      Further validation is required before this technique can be advocated for use in 
      clinical decision making.
CI  - (c) 2017 EVJ Ltd.
FAU - Jago, R C
AU  - Jago RC
AUID- ORCID: 0000-0003-3471-5429
AD  - Royal (Dick) School of Veterinary Studies and Roslin Institute, University of 
      Edinburgh, Edinburgh, UK.
FAU - Scholes, S
AU  - Scholes S
AD  - SAC Consulting Veterinary Services, Penicuik, UK.
FAU - Mair, T S
AU  - Mair TS
AD  - Bell Equine Veterinary Clinic, Maidstone, UK.
FAU - Pearson, G R
AU  - Pearson GR
AD  - School of Clinical Veterinary Science, University of Bristol, Bristol, UK.
FAU - Pirie, R S
AU  - Pirie RS
AD  - Royal (Dick) School of Veterinary Studies and Roslin Institute, University of 
      Edinburgh, Edinburgh, UK.
FAU - Handel, I
AU  - Handel I
AD  - Royal (Dick) School of Veterinary Studies and Roslin Institute, University of 
      Edinburgh, Edinburgh, UK.
FAU - Milne, E M
AU  - Milne EM
AD  - Royal (Dick) School of Veterinary Studies and Roslin Institute, University of 
      Edinburgh, Edinburgh, UK.
FAU - Coyle, F
AU  - Coyle F
AD  - Royal (Dick) School of Veterinary Studies and Roslin Institute, University of 
      Edinburgh, Edinburgh, UK.
FAU - Mcgorum, B C
AU  - Mcgorum BC
AD  - Royal (Dick) School of Veterinary Studies and Roslin Institute, University of 
      Edinburgh, Edinburgh, UK.
LA  - eng
PT  - Journal Article
DEP - 20170818
PL  - United States
TA  - Equine Vet J
JT  - Equine veterinary journal
JID - 0173320
RN  - 0 (Amyloid beta-Peptides)
SB  - IM
MH  - Amyloid beta-Peptides/chemistry/*metabolism
MH  - Animals
MH  - Autonomic Nervous System Diseases
MH  - Biopsy
MH  - Case-Control Studies
MH  - Horse Diseases/*diagnosis
MH  - Horses
MH  - Immunohistochemistry/*veterinary
MH  - Neurons/metabolism
MH  - Rectum/*pathology
MH  - Retrospective Studies
OTO - NOTNLM
OT  - dysautonomia
OT  - grass sickness
OT  - horse
OT  - neurodegeneration
OT  - rectal biopsy
OT  - beta-amyloid precursor protein
EDAT- 2017/06/18 06:00
MHDA- 2017/12/27 06:00
CRDT- 2017/06/17 06:00
PHST- 2016/09/16 00:00 [received]
PHST- 2017/06/10 00:00 [accepted]
PHST- 2017/06/18 06:00 [pubmed]
PHST- 2017/12/27 06:00 [medline]
PHST- 2017/06/17 06:00 [entrez]
AID - 10.1111/evj.12710 [doi]
PST - ppublish
SO  - Equine Vet J. 2018 Jan;50(1):22-28. doi: 10.1111/evj.12710. Epub 2017 Aug 18.