PMID- 28622482
OWN - NLM
STAT- MEDLINE
DCOM- 20180117
LR  - 20180117
IS  - 1557-7600 (Electronic)
IS  - 1096-620X (Linking)
VI  - 20
IP  - 9
DP  - 2017 Sep
TI  - Antidiabetic Properties of an Apple/Kale Extract In Vitro, In Situ, and in Mice 
      Fed a Western-Type Diet.
PG  - 846-854
LID - 10.1089/jmf.2017.0019 [doi]
AB  - Type 2 diabetes mellitus (T2DM) is a common and increasingly prevalent metabolic 
      disorder, and effective preventive strategies against this disease are needed. 
      The aim of the present study was to evaluate the potential antidiabetic 
      properties of a dietary apple/kale extract (AKE), which was rich in phlorizin and 
      flavonoids, in laboratory mice. Mice were fed a control diet, a Western-type 
      high-sugar, high-fat diet (WTD), or a WTD plus AKE for 10 weeks. Body weight, 
      food and energy intake, body composition, and blood glucose level were recorded 
      in addition to the postprandial rise in blood glucose concentration after a 
      single administration of glucose (oral glucose tolerance test, OGTT). 
      Furthermore, changes in glucose-induced short-circuit current (I(SC)) in response 
      to AKE and phlorizin administration were evaluated in situ in intestinal tissues 
      with Ussing chambers. In addition, the in vitro inhibition of alpha-glucosidase by 
      AKE was determined. The present data suggest that supplementation of an AKE to a 
      WTD significantly improved both blood glucose levels and OGTT in mice. 
      Furthermore, in situ uptake of glucose was significantly inhibited by AKE. 
      Finally, we showed that AKE significantly inhibits alpha-glucosidase activity in 
      vitro. We conclude that AKE exhibits antidiabetic properties by a dual mechanism, 
      including the inhibition of alpha-glucosidase and sodium-dependent glucose 
      transporter 1 (SGLT1). Thus, AKE has the potential to serve as a natural plant 
      bioactive compound for dietary prevention strategies against T2DM.
FAU - Schloesser, Anke
AU  - Schloesser A
AD  - 1 Institute of Human Nutrition and Food Science, University of Kiel , Kiel, 
      Germany .
FAU - Esatbeyoglu, Tuba
AU  - Esatbeyoglu T
AD  - 1 Institute of Human Nutrition and Food Science, University of Kiel , Kiel, 
      Germany .
FAU - Schultheiss, Gerhard
AU  - Schultheiss G
AD  - 2 Animal Welfare Officer, University of Kiel , Kiel, Germany .
FAU - Vollert, Henning
AU  - Vollert H
AD  - 3 BioActive Food GmbH , Bad Segeberg, Germany .
FAU - Luersen, Kai
AU  - Luersen K
AD  - 1 Institute of Human Nutrition and Food Science, University of Kiel , Kiel, 
      Germany .
FAU - Fischer, Alexandra
AU  - Fischer A
AD  - 1 Institute of Human Nutrition and Food Science, University of Kiel , Kiel, 
      Germany .
FAU - Rimbach, Gerald
AU  - Rimbach G
AD  - 1 Institute of Human Nutrition and Food Science, University of Kiel , Kiel, 
      Germany .
LA  - eng
PT  - Journal Article
DEP - 20170616
PL  - United States
TA  - J Med Food
JT  - Journal of medicinal food
JID - 9812512
RN  - 0 (Blood Glucose)
RN  - 0 (Flavonoids)
RN  - 0 (Hypoglycemic Agents)
RN  - 0 (Plant Extracts)
RN  - 0 (Slc5a1 protein, mouse)
RN  - 0 (Sodium-Glucose Transporter 1)
RN  - CU9S17279X (Phlorhizin)
RN  - EC 3.2.1.20 (alpha-Glucosidases)
SB  - IM
MH  - Animals
MH  - Blood Glucose/metabolism
MH  - Brassica/*chemistry
MH  - Diabetes Mellitus, Type 2/*drug therapy/genetics/metabolism
MH  - Diet, High-Fat/adverse effects
MH  - Female
MH  - Flavonoids/administration & dosage
MH  - Glucose Tolerance Test
MH  - Humans
MH  - Hypoglycemic Agents/*administration & dosage
MH  - Male
MH  - Malus/*chemistry
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Phlorhizin/administration & dosage
MH  - Plant Extracts/*administration & dosage
MH  - Sodium-Glucose Transporter 1/genetics/metabolism
MH  - alpha-Glucosidases/genetics/metabolism
OTO - NOTNLM
OT  - SGLT1
OT  - flavonoids
OT  - phlorizin
OT  - type 2 diabetes mellitus
OT  - alpha-glucosidase
EDAT- 2017/06/18 06:00
MHDA- 2018/01/18 06:00
CRDT- 2017/06/17 06:00
PHST- 2017/06/18 06:00 [pubmed]
PHST- 2018/01/18 06:00 [medline]
PHST- 2017/06/17 06:00 [entrez]
AID - 10.1089/jmf.2017.0019 [doi]
PST - ppublish
SO  - J Med Food. 2017 Sep;20(9):846-854. doi: 10.1089/jmf.2017.0019. Epub 2017 Jun 16.