PMID- 28623647
OWN - NLM
STAT- MEDLINE
DCOM- 20181116
LR  - 20211204
IS  - 1724-6016 (Electronic)
IS  - 1120-6721 (Linking)
VI  - 27
IP  - 6
DP  - 2017 Nov 8
TI  - Expression of mTOR in Primary Pterygium and its Correlation with alpha-Smooth Muscle 
      Actin.
PG  - 664-669
LID - 10.5301/ejo.5000985 [doi]
AB  - PURPOSE: Mammalian target of rapamycin (mTOR) is a serine/threonine protein 
      kinase that has been shown to affect many cellular functions, such as cell 
      growth, proliferation, and metabolism. However, there has been minimal focus on 
      the expression of mTOR in pterygium. The purpose of this study was to investigate 
      the expression of mTOR and the correlation between the levels of mTOR and 
      alpha-smooth muscle actin (alpha-SMA, a marker of transdifferentiation) in pterygium. 
      METHODS: Primary pterygium samples from 28 patients and normal conjunctival 
      samples from 16 patients were surgically removed and analyzed. The expression 
      levels of mTOR and alpha-SMA in the excised specimens were assessed using 
      immunohistochemistry and Western blotting. Furthermore, correlations between the 
      mTOR and alpha-SMA expression levels were studied. RESULTS: The expression of mTOR 
      and alpha-SMA was significantly higher in the pterygium tissues than in normal 
      conjunctiva tissues. A significant positive correlation was detected between the 
      number of mTOR-immunopositive fibroblasts and the number of alpha-SMA-immunopositive 
      fibroblasts (rho = 0.463, p = 0.0078). Additionally, mTOR expression was 
      significantly correlated with alpha-SMA expression (rho = 0.269, p = 0.031) in 
      pterygium. CONCLUSIONS: There was an increased expression of mTOR in pterygium 
      samples compared to that in normal conjunctival tissues, with a positive 
      correlation with alpha-SMA expression. These findings might be involved in the 
      pathogenesis of pterygium.
FAU - Zhao, Xin-Rong
AU  - Zhao XR
AD  - 1 Department of Ophthalmology, Union Hospital, Tongji Medical College of Huazhong 
      University of Science and Technology, Wuhan - China.
AD  - 2 Department of Ophthalmology, the First Affiliated Hospital of Medical College, 
      Shihezi University, Shihezi, Xin Jiang - China.
FAU - Zhang, Ming-Chang
AU  - Zhang MC
AD  - 1 Department of Ophthalmology, Union Hospital, Tongji Medical College of Huazhong 
      University of Science and Technology, Wuhan - China.
FAU - Xie, Hua-Tao
AU  - Xie HT
AD  - 1 Department of Ophthalmology, Union Hospital, Tongji Medical College of Huazhong 
      University of Science and Technology, Wuhan - China.
FAU - Ji, Nan
AU  - Ji N
AD  - 2 Department of Ophthalmology, the First Affiliated Hospital of Medical College, 
      Shihezi University, Shihezi, Xin Jiang - China.
FAU - Ma, Xiao-Qiong
AU  - Ma XQ
AD  - 3 Department of Ophthalmology, Yili Friendship Hospital, Yili, Xin Jiang - China.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Eur J Ophthalmol
JT  - European journal of ophthalmology
JID - 9110772
RN  - 0 (ACTA2 protein, human)
RN  - 0 (Actins)
RN  - 0 (Biomarkers)
RN  - EC 2.7.1.1 (MTOR protein, human)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
SB  - IM
MH  - Actins/*metabolism
MH  - Aged
MH  - Biomarkers/metabolism
MH  - Blotting, Western
MH  - Female
MH  - Fibroblasts/metabolism
MH  - Humans
MH  - Immunohistochemistry
MH  - Male
MH  - Middle Aged
MH  - Pterygium/*metabolism/pathology
MH  - TOR Serine-Threonine Kinases/*metabolism
OTO - NOTNLM
OT  - Pterygium
OT  - Pterygium fibroblasts
OT  - mTOR
OT  - alpha-Smooth muscle actin
EDAT- 2017/06/18 06:00
MHDA- 2018/11/18 06:00
CRDT- 2017/06/18 06:00
PHST- 2017/06/18 06:00 [pubmed]
PHST- 2018/11/18 06:00 [medline]
PHST- 2017/06/18 06:00 [entrez]
AID - 48ECE766-0F29-4318-A5FC-E715EE335544 [pii]
AID - 10.5301/ejo.5000985 [doi]
PST - ppublish
SO  - Eur J Ophthalmol. 2017 Nov 8;27(6):664-669. doi: 10.5301/ejo.5000985.