PMID- 28624282
OWN - NLM
STAT- MEDLINE
DCOM- 20180420
LR  - 20181202
IS  - 1525-3198 (Electronic)
IS  - 0022-0302 (Linking)
VI  - 100
IP  - 8
DP  - 2017 Aug
TI  - Effect of endoplasmic reticulum stress on metabolic and stress signaling and 
      kidney-specific functions in Madin-Darby bovine kidney cells.
PG  - 6689-6706
LID - S0022-0302(17)30591-X [pii]
LID - 10.3168/jds.2016-12406 [doi]
AB  - Recent studies demonstrated induction of endoplasmic reticulum (ER) stress in 
      tissues of cows after parturition, but knowledge about the effect of ER stress on 
      important cellular processes, such as critical signaling and metabolic pathways, 
      in cattle is scarce. Thus, the present study aimed to investigate the effect of 
      ER stress induction on nuclear factor-kappaB (NF-kappaB), nuclear factor E2-related 
      factor 2 (Nrf2), and sterol regulatory element-binding protein (SREBF1) pathway 
      in Madin-Darby bovine kidney (MDBK) cells, a widely used in vitro model in 
      ruminant research. To consider the kidney origin of MDBK cells, the effect on 
      renal distal tubular cell-specific functions, such as transport processes and 
      regulation of 1,25(OH)(2)D(3) levels, was also studied. Treatment of MDBK cells 
      with 2 different ER stress inducers, thapsigargin (TG) and tunicamycin (TM), 
      strongly induced ER stress as evident from induction of ER stress target genes, 
      increased phosphorylation of PKR-like ER kinase, and enhanced splicing of X-box 
      binding protein 1. The TM decreased the protein concentration of NF-kappaB p50 and 
      the mRNA levels of the NF-kappaB target genes. Likewise, TG decreased the mRNA 
      concentration of tumor necrosis factor and tended to decrease NF-kappaB p50 protein 
      and mRNA levels of NF-kappaB target genes. The mRNA levels of most of the Nrf2 target 
      genes investigated were reduced by TG and TM in MDBK cells. Both ER stress 
      inducers reduced the mRNA levels of SREBF1 and its target genes in MDBK cells. 
      Interestingly, TG decreased, but TM increased the mRNA level of the Ca(2+) 
      binding protein calbindin 1, whereas the mRNA level of the plasma membrane 
      Ca(2+)-transporting ATPase 1 remained unchained. The mRNA level of the cytochrome 
      P450 component 24A1 involved in 1alpha-hydroxylation of 25(OH)D(3) was strongly 
      elevated, whereas the mRNA level of the cytochrome P450 component 27A1 catalyzing 
      the breakdown of 1,25(OH)(2)D(3) was markedly reduced by both ER stress inducers. 
      The concentration of 1,25(OH)(2)D(3) in the supernatant of MDBK cells was 
      increased by approximately 15% by both TG and TM. The present study indicates 
      that under conditions of ER stress, critical signaling pathways, such as NF-kappaB, 
      Nrf2, and SREBF1, are inhibited, whereas the formation of 1,25(OH)(2)D(3) is 
      stimulated in bovine MDBK cells. Future studies are necessary to clarify the 
      physiological relevance of these findings.
CI  - Copyright (c) 2017 American Dairy Science Association. Published by Elsevier Inc. 
      All rights reserved.
FAU - Chiappisi, E
AU  - Chiappisi E
AD  - Institute of Animal Nutrition and Nutrition Physiology, Justus-Liebig-University 
      Giessen, Heinrich-Buff-Ring 26-32, 35392 Giessen, Germany.
FAU - Ringseis, R
AU  - Ringseis R
AD  - Institute of Animal Nutrition and Nutrition Physiology, Justus-Liebig-University 
      Giessen, Heinrich-Buff-Ring 26-32, 35392 Giessen, Germany.
FAU - Eder, K
AU  - Eder K
AD  - Institute of Animal Nutrition and Nutrition Physiology, Justus-Liebig-University 
      Giessen, Heinrich-Buff-Ring 26-32, 35392 Giessen, Germany.
FAU - Gessner, D K
AU  - Gessner DK
AD  - Institute of Animal Nutrition and Nutrition Physiology, Justus-Liebig-University 
      Giessen, Heinrich-Buff-Ring 26-32, 35392 Giessen, Germany. Electronic address: 
      denise.gessner@ernaehrung.uni-giessen.de.
LA  - eng
PT  - Journal Article
DEP - 20170616
PL  - United States
TA  - J Dairy Sci
JT  - Journal of dairy science
JID - 2985126R
RN  - 0 (NF-kappa B)
RN  - 0 (X-Box Binding Protein 1)
RN  - 11089-65-9 (Tunicamycin)
RN  - 67526-95-8 (Thapsigargin)
SB  - IM
MH  - Animals
MH  - Cattle
MH  - Cell Line
MH  - *Endoplasmic Reticulum Stress
MH  - Female
MH  - Kidney Tubules, Distal
MH  - NF-kappa B
MH  - *Signal Transduction
MH  - Thapsigargin/pharmacology
MH  - Tunicamycin/pharmacology
MH  - X-Box Binding Protein 1/*metabolism
OTO - NOTNLM
OT  - Madin-Darby bovine kidney cell
OT  - endoplasmic reticulum stress
OT  - inflammation
OT  - vitamin D
EDAT- 2017/06/19 06:00
MHDA- 2018/04/21 06:00
CRDT- 2017/06/19 06:00
PHST- 2016/12/06 00:00 [received]
PHST- 2017/04/28 00:00 [accepted]
PHST- 2017/06/19 06:00 [pubmed]
PHST- 2018/04/21 06:00 [medline]
PHST- 2017/06/19 06:00 [entrez]
AID - S0022-0302(17)30591-X [pii]
AID - 10.3168/jds.2016-12406 [doi]
PST - ppublish
SO  - J Dairy Sci. 2017 Aug;100(8):6689-6706. doi: 10.3168/jds.2016-12406. Epub 2017 
      Jun 16.